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To identify the coexpression and the expression levels of regulatory genes during hematopoietic stem cell (HSC) differentiation toward the T cell branch, we applied a new single-cell RT-PCR technique to analyze the simultaneous expression of 13 genes in 9 functionally purified populations from the bone marrow and the thymus. We report in this paper that Lin\u2212Sca1+ckit+ HSCs display, at the single-cell level, a homogeneous and high transcriptional activity as do early thymic progenitors. Moreover, the coexpression of lymphoid and myeloid genes is an early event detected in \u223c30% of short-term HSC and most multipotent progenitors, suggesting novel sources for the generation of early thymic progenitors, common lymphoid progenitors (CLPs), and common myeloid progenitors. Loss of multipotency in Lin\u2212Sca1+ckit+ cells directed to the lymphoid branch is characterized by Lmo2 and Gata2 gene expression downregulation. Indeed, highest levels of Gata2 expression are detected only in long-term and short-term HSC populations. Complete shutdown of Pu1 gene expression in all triple-negative (TN)3 stage thymic pre-T cells is indicative of total T cell commitment. Interestingly, this is also observed in 30% of TN2 cells and 25% of CLP in the bone marrow, suggesting a possible initiation of T cell engagement in TN2 and CLP. Also, our strategy highlights similar gene patterns among HSCs and intrathymic progenitors, proposing, therefore, that identical activation signals are maintained until further maturation and generation of CD4 and CD8 coreceptors bearing thymocytes.<\/jats:p>","DOI":"10.4049\/jimmunol.0902184","type":"journal-article","created":{"date-parts":[[2010,4,5]],"date-time":"2010-04-05T20:45:03Z","timestamp":1270500303000},"page":"4907-4917","source":"Crossref","is-referenced-by-count":8,"title":["Gene Coexpression Analysis in Single Cells Indicates Lymphomyeloid Copriming in Short-Term Hematopoietic Stem Cells and Multipotent Progenitors"],"prefix":"10.1093","volume":"184","author":[{"given":"Laetitia","family":"Gautreau","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale , Unit\u00e9 591"},{"name":"Universit\u00e9 Paris Descartes , Unit\u00e9 Mixte de Recherche S591"}]},{"given":"Amine","family":"Boudil","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale , Unit\u00e9 591"},{"name":"Universit\u00e9 Paris Descartes , Unit\u00e9 Mixte de Recherche S591"}]},{"given":"Val\u00e9rie","family":"Pasqualetto","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale , Unit\u00e9 591"},{"name":"Universit\u00e9 Paris Descartes , Unit\u00e9 Mixte de Recherche S591"}]},{"given":"Lamia","family":"Skhiri","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale , Unit\u00e9 591"},{"name":"Universit\u00e9 Paris Descartes , Unit\u00e9 Mixte de Recherche S591"}]},{"given":"Laure","family":"Grandin","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris, Site Necker-Enfants-Malades, Service de Biostatistique et Informatique M\u00e9dicale , Paris,","place":["France"]}]},{"given":"Marta","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale , Unit\u00e9 591"},{"name":"Universit\u00e9 Paris Descartes , Unit\u00e9 Mixte de Recherche S591"}]},{"given":"Jean-Philippe","family":"Jais","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris, Site Necker-Enfants-Malades, Service de Biostatistique et Informatique M\u00e9dicale , Paris,","place":["France"]}]},{"given":"Sophie","family":"Ezine","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale , Unit\u00e9 591"},{"name":"Universit\u00e9 Paris Descartes , Unit\u00e9 Mixte de Recherche S591"}]}],"member":"286","published-online":{"date-parts":[[2010,5,1]]},"reference":[{"key":"2026012808083038000_r1","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/S0092-8674(00)80453-5","article-title":"Identification of clonogenic common lymphoid progenitors in mouse bone marrow.","volume":"91","author":"Kondo","year":"1997","journal-title":"Cell"},{"key":"2026012808083038000_r2","doi-asserted-by":"crossref","first-page":"3333","DOI":"10.1182\/blood.V97.11.3333","article-title":"Dendritic cell potentials of early lymphoid and myeloid progenitors.","volume":"97","author":"Manz","year":"2001","journal-title":"Blood"},{"key":"2026012808083038000_r3","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.cell.2005.02.013","article-title":"Identification of Flt3+ lympho-myeloid stem cells lacking erythro-megakaryocytic potential a revised road map for adult blood lineage commitment.","volume":"121","author":"Adolfsson","year":"2005","journal-title":"Cell"},{"key":"2026012808083038000_r4","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/S1074-7613(00)80105-3","article-title":"Notch1 expression in early lymphopoiesis influences B versus T lineage determination.","volume":"11","author":"Pui","year":"1999","journal-title":"Immunity"},{"key":"2026012808083038000_r5","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/S1074-7613(00)80054-0","article-title":"Deficient T cell fate specification in mice with an induced inactivation of Notch1.","volume":"10","author":"Radtke","year":"1999","journal-title":"Immunity"},{"key":"2026012808083038000_r6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1084\/jem.20061442","article-title":"Hierarchy of Notch-Delta interactions promoting T cell lineage commitment and maturation.","volume":"204","author":"Besseyrias","year":"2007","journal-title":"J. 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