{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T14:44:09Z","timestamp":1751035449415,"version":"3.38.0"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>IFN-\u03b3 is known to be predominantly produced by lymphoid cells such as certain subsets of T cells, NK cells, and other group 1 innate lymphoid cells. In this study, we used IFN-\u03b3 reporter mouse models to search for additional cells capable of secreting this cytokine. We identified a novel and rare population of nonconventional IFN-\u03b3\u2013producing cells of hematopoietic origin that were characterized by the expression of Thy1.2 and the lack of lymphoid, myeloid, and NK lineage markers. The expression of IFN-\u03b3 by this population was higher in the liver and lower in the spleen. Furthermore, these cells were present in mice lacking both the Rag2 and the common \u03b3-chain (\u03b3c) genes (Rag2\u2212\/\u2212\u03b3c\u2212\/\u2212), indicating their innate nature and their \u03b3c cytokine independence. Rag2\u2212\/\u2212\u03b3c\u2212\/\u2212 mice are as resistant to Mycobacterium avium as Rag2\u2212\/\u2212 mice, whereas Rag2\u2212\/\u2212 mice lacking IFN-\u03b3 are more susceptible than either Rag2\u2212\/\u2212 or Rag2\u2212\/\u2212\u03b3c\u2212\/\u2212. These lineage-negative CD45+\/Thy1.2+ cells are found within the mycobacterially induced granulomatous structure in the livers of infected Rag2\u2212\/\u2212\u03b3c\u2212\/\u2212 animals and are adjacent to macrophages that expressed inducible NO synthase, suggesting a potential protective role for these IFN-\u03b3\u2013producing cells. Accordingly, Thy1.2-specific mAb administration to infected Rag2\u2212\/\u2212\u03b3c\u2212\/\u2212 animals increased M. avium growth in the liver. Overall, our results demonstrate that a population of Thy1.2+ non-NK innate-like cells present in the liver expresses IFN-\u03b3 and can confer protection against M. avium infection in immunocompromised mice.<\/jats:p>","DOI":"10.4049\/jimmunol.1601701","type":"journal-article","created":{"date-parts":[[2017,7,8]],"date-time":"2017-07-08T00:30:25Z","timestamp":1499473825000},"page":"1429-1439","source":"Crossref","is-referenced-by-count":9,"title":["Innate IFN-\u03b3\u2013Producing Cells Developing in the Absence of IL-2 Receptor Common \u03b3-Chain"],"prefix":"10.1093","volume":"199","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4835-3035","authenticated-orcid":false,"given":"Mariana","family":"Resende","sequence":"first","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]},{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho and ICVS\/3B\u2019s \u2013 PT Government Associate Laboratory , 4170 Braga\/Guimar\u00e3es,","place":["Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0150-7359","authenticated-orcid":false,"given":"Marcos S","family":"Cardoso","sequence":"additional","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]}]},{"given":"Ana R","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]}]},{"given":"Manuela","family":"Fl\u00f3rido","sequence":"additional","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6035-4095","authenticated-orcid":false,"given":"Margarida","family":"Borges","sequence":"additional","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]}]},{"given":"Ant\u00f3nio Gil","family":"Castro","sequence":"additional","affiliation":[{"name":"Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho and ICVS\/3B\u2019s \u2013 PT Government Associate Laboratory , 4170 Braga\/Guimar\u00e3es,","place":["Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1567-8389","authenticated-orcid":false,"given":"Nuno L","family":"Alves","sequence":"additional","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6050-3863","authenticated-orcid":false,"given":"Andrea M","family":"Cooper","sequence":"additional","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9447-0665","authenticated-orcid":false,"given":"Rui","family":"Appelberg","sequence":"additional","affiliation":[{"name":"IBMC \u2013 Instituto de Biologia Molecular e Celular and i3S \u2013 Instituto de Investiga\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , 4200-135 Porto,","place":["Portugal"]}]}],"member":"286","published-online":{"date-parts":[[2017,8,15]]},"reference":[{"key":"2025030614243933600_r1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/S0065-2776(07)96002-2","article-title":"Regulation of interferon-gamma during innate and adaptive immune responses","volume":"96","author":"Schoenborn","year":"2007","journal-title":"Adv. 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