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Lectin blot and precipitation analysis of CSF revealed that PVL (<jats:italic>Psathyrella velutina<\/jats:italic> lectin) bound an unique N\u2010acetylglucosamine\u2010terminated N\u2010glycans on \u201cCSF\u2010type\u201d Tf whereas SSA (<jats:italic>Sambucus sieboldiana<\/jats:italic> agglutinin) bound <jats:italic>\u03b1<\/jats:italic>2,6\u2010N\u2010acetylneuraminic acid\u2010terminated N\u2010glycans on \u201cserum\u2010type\u201d Tf. PVL\u2010TfAb ELISA of 0.5\u2009<jats:italic>\u03bc<\/jats:italic>L CSF samples detected \u201cCSF\u2010type\u201d Tf but not \u201cserum\u2010type\u201d Tf whereas SSA\u2010TfAb ELISA detected \u201cserum\u2010type\u201d Tf but not \u201cCSF\u2010type\u201d Tf, demonstrating the specificity of the lectin\u2010TfAb ELISAs. In idiopathic normal pressure hydrocephalus (iNPH), a senile dementia associated with ventriculomegaly, amounts of the SSA\u2010reactive Tf were significantly higher than in non\u2010iNPH patients, indicating that Tf glycan analysis by the high\u2010throughput lectin\u2010TfAb ELISAs could become practical diagnostic tools for iNPH. The lectin\u2010antibody ELISAs of CSF proteins might be useful for diagnosis of the other neurological diseases.<\/jats:p>","DOI":"10.4061\/2011\/352787","type":"journal-article","created":{"date-parts":[[2011,8,25]],"date-time":"2011-08-25T12:15:55Z","timestamp":1314274555000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["High Throughput ELISAs to Measure a Unique Glycan on Transferrin in Cerebrospinal Fluid: A Possible Extension toward Alzheimer\u2032s Disease Biomarker Development"],"prefix":"10.1155","volume":"2011","author":[{"given":"Keiro","family":"Shirotani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Futakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyomitsu","family":"Nara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoka","family":"Hoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshie","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuriko","family":"Tohyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinobu","family":"Kitazume","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuhiko","family":"Yuasa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Miyajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Kuno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hisashi","family":"Narimatsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiro","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,8,17]]},"reference":[{"key":"e_1_2_7_1_2","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1155\/2005\/848676","article-title":"The use of proteomics in biomarker discovery in neurodegenerative diseases","volume":"21","author":"Davidsson P.","year":"2005","journal-title":"Disease Markers"},{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00701\u2010006\u20100787\u20104"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","unstructured":"FutakawaS. 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