{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T01:09:43Z","timestamp":1775869783261,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2011,10,5]],"date-time":"2011-10-05T00:00:00Z","timestamp":1317772800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Research International"],"published-print":{"date-parts":[[2011,10,5]]},"abstract":"<jats:p>Recombinant antibody phage display technology is a vital tool that facilitates identification of specific binding molecules to a target enabling the rapid generation and selection of high affinity, fully human, or mouse antibody product candidates essentially directed towards disease target appropriate for antibody therapy. In this study, a recombinant single-chain Fv antibody fragment (scFv) A11 was isolated from immune spleen cells obtained from mice immunized with inactivated rabies virus (Pasteur strain) using standard methodology and was characterized for its specificity towards the rabies virus glycoprotein. Epitope mapping using peptide libraries and truncated glycoprotein polypeptides suggested that A11 bound to the antigenic site II of rabies glycoprotein against which a majority of rabies virus neutralizing antibodies are directed. The use of the above technology could, therefore, allow development of scFvs with different specificities against the rabies glycoprotein as an alternative to the more cumbersome protocols used for the development of monoclonal antibodies.<\/jats:p>","DOI":"10.4061\/2011\/652147","type":"journal-article","created":{"date-parts":[[2011,10,5]],"date-time":"2011-10-05T19:03:36Z","timestamp":1317841416000},"page":"1-11","source":"Crossref","is-referenced-by-count":9,"title":["Generation and Characterization of an scFv Directed against Site II of Rabies Glycoprotein"],"prefix":"10.4061","volume":"2011","author":[{"given":"Shukra M.","family":"Aavula","sequence":"first","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sridevi V.","family":"Nimmagadda","sequence":"additional","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6094-8376","authenticated-orcid":true,"given":"Neelakantam","family":"Biradhar","sequence":"additional","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel","family":"Sula","sequence":"additional","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dev","family":"Chandran","sequence":"additional","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendra","family":"Lingala","sequence":"additional","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srinivasan Alwar","family":"Villuppanoor","sequence":"additional","affiliation":[{"name":"Research and Development Center, Indian Immunologicals Limited, Rakshapuram, Gachibowli, Hyderabad 500032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"2","key":"1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1128\/CMR.9.2.166","volume":"9","year":"1996","journal-title":"Clinical Microbiology Reviews"},{"issue":"18","key":"2","first-page":"879","volume":"125","year":"1995","journal-title":"Schweizerische Medizinische Wochenschrift"},{"issue":"8","key":"3","doi-asserted-by":"crossref","first-page":"833","DOI":"10.3201\/eid0808.010516","volume":"8","year":"2002","journal-title":"Emerging Infectious Diseases"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1086\/324628"},{"issue":"4","key":"5","first-page":"238","volume":"103","year":"2005","journal-title":"Journal of the Indian Medical Association"},{"issue":"6","key":"6","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1073\/pnas.88.6.2432","volume":"88","year":"1991","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"4705","key":"7","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1126\/science.4001944","volume":"228","year":"1985","journal-title":"Science"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nbt785"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.24.14130"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1038\/nm0996-979"},{"issue":"5187","key":"11","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1126\/science.7973652","volume":"266","year":"1994","journal-title":"Science"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/348552a0"},{"issue":"3","key":"13","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1136\/mp.53.3.111","volume":"53","year":"2000","journal-title":"Molecular Pathology"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.12.5548-5555.2000"},{"key":"15","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1038\/nbt1142","volume":"23","year":"2005","journal-title":"Nature Biotechnology"},{"issue":"3","key":"16","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1016\/0022-2836(91)90498-U","volume":"222","year":"1991","journal-title":"Journal of Molecular Biology"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1038\/352624a0"},{"key":"18","first-page":"10832","volume":"12","year":"1991","journal-title":"Biochemistry"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00204-10"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.biologicals.2005.07.004"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(98)80121-8"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.2.669"},{"issue":"5","key":"24","first-page":"991","volume":"58","year":"1998","journal-title":"Cancer Research"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(97)00199-9"},{"issue":"1","key":"26","first-page":"1","volume":"45","year":"1971","journal-title":"Bulletin of the World Health Organization"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2335"},{"key":"28","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1146\/annurev.iy.12.040194.002245","volume":"12","year":"1994","journal-title":"Annual Review of Immunology"},{"issue":"10","key":"29","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1093\/protein\/12.10.873","volume":"12","year":"1999","journal-title":"Protein Engineering"},{"issue":"12","key":"30","doi-asserted-by":"crossref","first-page":"6953","DOI":"10.1073\/pnas.96.12.6953","volume":"96","year":"1999","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"8","key":"31","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.1128\/JVI.65.8.4198-4203.1991","volume":"65","year":"1991","journal-title":"Journal of Virology"},{"issue":"1","key":"32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/JVI.62.1.1-7.1988","volume":"62","year":"1988","journal-title":"Journal of Virology"},{"issue":"3","key":"33","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1128\/JVI.53.3.926-934.1985","volume":"53","year":"1985","journal-title":"Journal of Virology"},{"issue":"11","key":"34","doi-asserted-by":"crossref","first-page":"3914","DOI":"10.1073\/pnas.83.11.3914","volume":"83","year":"1986","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"4967","key":"35","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1126\/science.1696028","volume":"249","year":"1990","journal-title":"Science"},{"issue":"4","key":"36","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1099\/0022-1317-64-4-843","volume":"64","year":"1983","journal-title":"Journal of General Virology"},{"issue":"1","key":"37","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1128\/JVI.72.1.273-278.1998","volume":"72","year":"1998","journal-title":"Journal of Virology"},{"issue":"1","key":"38","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1073\/pnas.80.1.70","volume":"80","year":"1983","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"issue":"2","key":"39","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1099\/0022-1317-70-2-291","volume":"70","year":"1989","journal-title":"Journal of General Virology"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(97)00080-4"},{"issue":"9","key":"41","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1111\/j.1348-0421.1995.tb03258.x","volume":"39","year":"1995","journal-title":"Microbiology and Immunology"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.16.6378"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(91)90213-P"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.4158"}],"container-title":["Biotechnology Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/archive\/2011\/652147.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2011\/652147.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/archive\/2011\/652147.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,12,8]],"date-time":"2020-12-08T23:49:03Z","timestamp":1607471343000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/btri\/2011\/652147\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,5]]},"references-count":44,"alternative-id":["652147","652147"],"URL":"https:\/\/doi.org\/10.4061\/2011\/652147","relation":{},"ISSN":["2090-3138","2090-3146"],"issn-type":[{"value":"2090-3138","type":"print"},{"value":"2090-3146","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,5]]}}}