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In this study, using rat sublingual gland acinar cells, we report that<jats:italic>P. gingivalis<\/jats:italic>LPS-induced impairment in mucin synthesis and associated suppression in Akt kinase activity were accompanied by a decrease in constitutive nitric oxide synthase (cNOS) activity and an induction in inducible nitric oxide synthase (iNOS) expression. The LPS effect on Akt inactivation was manifested in the kinase S-nitrosylation and a decrease in its phosphorylation at Ser<jats:sup>473<\/jats:sup>. Further, we demonstrate that a peptide hormone, ghrelin, countered the LPS-induced impairment in mucin synthesis. This effect of ghrelin was reflected in the suppression of iNOS and the increase in Akt activation, associated with the loss in S-nitrosylation and the increase in phosphorylation, as well as cNOS activation through phosphorylation. Our findings suggest that induction in iNOS expression by<jats:italic>P. gingivalis<\/jats:italic>-LPS leads to Akt kinase inactivation through S-nitrosylation that detrimentally impacts cNOS activation through phosphorylation as well as mucin synthesis. We also show that the countering effect of ghrelin on<jats:italic>P. gingivalis<\/jats:italic>-induced impairment in mucin synthesis is associated with Akt activation through phosphorylation.<\/jats:p>","DOI":"10.4061\/2011\/807279","type":"journal-article","created":{"date-parts":[[2011,4,6]],"date-time":"2011-04-06T18:03:00Z","timestamp":1302112980000},"page":"1-8","source":"Crossref","is-referenced-by-count":2,"title":["Ghrelin Protects against the Detrimental Consequences of<i>Porphyromonas gingivalis<\/i>-Induced Akt Inactivation through S-Nitrosylation on Salivary Mucin Synthesis"],"prefix":"10.4061","volume":"2011","author":[{"given":"Bronislaw L.","family":"Slomiany","sequence":"first","affiliation":[{"name":"Research Center, University of Medicine and Dentistry of New Jersey, P.O. 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