{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:25:22Z","timestamp":1793139922540,"version":"4.5.0"},"reference-count":54,"publisher":"PAGEPress Publications","issue":"2","license":[{"start":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T00:00:00Z","timestamp":1656547200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Ital Urol Androl"],"abstract":"<jats:p>Objective: Chronic genitourinary infections can alter male fertility and even promote carcinogenic processes. This study aimed to evaluate the effect of the presence in the semen of microorganisms on semen quality. Materials and methods: Clinical symptoms and conventional and functional seminal parameters of eleven fertile donors and ten volunteers with prostatitis-like symptoms were evaluated. Nitric oxide, antioxidant capacity, and pro-inflammatory cytokines in semen and seminal plasma samples were also quantified. Finally, the expression of the ROR-\u03b3T, FoxP3, and T-bet genes in semen and the presence of DNA of microorganisms associated with prostatitis in urine and semen were evaluated. Results: When compared with fertile donors, volunteers with chronic prostatitis-like symptoms reported erectile dysfunction (0% vs. 10%, p = 0.2825) and premature ejaculation (0% vs. 40%; p = 0.0190). No statistically significant differences were observed in seminal parameters, cytokine measurement, antioxidant capacity, nitric oxide concentration and ROR-\u03b3T, FoxP3, T-bet. Microorganisms responsible for sexually transmitted infections and some bacteria associated with the microbiota and infections in the prostate gland were detected. In the semen from the subjects with prostatitis-like symptoms T. vaginalis DNA was detected; in addition, N. gonorrhoeae DNA was also detected in semen and urine samples. S. pyogenes was detected in the urine samples from the control group. Conclusions: Prostatitis-like symptoms are a common finding in young men that affect sexual and reproductive health, but not always the seminal parameters or fertility. The presence of prostatitis- like symptoms does not affect seminal quality. However, it appears to be associated with an increased likelihood of erectile dysfunction and premature ejaculation. Thus, affecting the quality of life and sexual and reproductive health.<\/jats:p>","DOI":"10.4081\/aiua.2022.2.199","type":"journal-article","created":{"date-parts":[[2022,6,30]],"date-time":"2022-06-30T07:36:03Z","timestamp":1656574563000},"page":"199-205","source":"Crossref","is-referenced-by-count":8,"title":["Molecular analysis of microorganisms in the semen and their impact on semen parameters"],"prefix":"10.4081","volume":"94","author":[{"given":"Jenniffer","family":"Puerta Su\u00e1rez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Carlos","family":"Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0062-6444","authenticated-orcid":false,"given":"Walter Dario","family":"Cardona Maya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2022,6,30]]},"reference":[{"key":"54101","doi-asserted-by":"crossref","unstructured":"Miyake M, Ohnishi K, Hori S, et al. Mycoplasma genitalium infection and chronic inflammation in human prostate cancer: detection using prostatectomy and needle biopsy specimens. Cells. 2019; 8:212.","DOI":"10.3390\/cells8030212"},{"key":"54102","doi-asserted-by":"crossref","unstructured":"Xu C, Sun GF, Zhu YF, Wang YF. The correlation of Ureaplasma urealyticum infection with infertility. Andrologia. 1997; 29:219.","DOI":"10.1111\/j.1439-0272.1997.tb00319.x"},{"key":"54103","doi-asserted-by":"crossref","unstructured":"Verze P, Cai T, Lorenzetti S. The role of the prostate in male fertility, health and disease. Nat Rev Urol. 2016; 13:379.","DOI":"10.1038\/nrurol.2016.89"},{"key":"54104","doi-asserted-by":"crossref","unstructured":"Zhao Q, Yang F, Meng L, et al. Lycopene attenuates chronic prostatitis\/chronic pelvic pain syndrome by inhibiting oxidative stress and inflammation via the interaction of NF-kappaB, MAPKs, and Nrf2 signaling pathways in rats. Andrology. 2020; 8:747.","DOI":"10.1111\/andr.12747"},{"key":"54105","doi-asserted-by":"crossref","unstructured":"Fujita K, Ewing CM, Sokoll LJ, et al. Cytokine profiling of prostatic fluid from cancerous prostate glands identifies cytokines associated with extent of tumor and inflammation. Prostate. 2008; 68:872.","DOI":"10.1002\/pros.20755"},{"key":"54106","doi-asserted-by":"crossref","unstructured":"Zhang Q, Liu S, Parajuli KR, et al. Interleukin-17 promotes prostate cancer via MMP7-induced epithelial-to-mesenchymal transition. Oncogene. 2017; 36:687.","DOI":"10.1038\/onc.2016.240"},{"key":"54107","doi-asserted-by":"crossref","unstructured":"Chen J, Zhang H, Niu D, et al. The risk factors related to the severity of pain in patients with Chronic Prostatitis\/Chronic Pelvic Pain Syndrome. BMC Urol. 2020; 20:154.","DOI":"10.1186\/s12894-020-00729-9"},{"key":"54108","doi-asserted-by":"crossref","unstructured":"Farahani L, Tharakan T, Yap T, et al. The semen microbiome and its impact on sperm function and male fertility: A systematic review and meta-analysis. Andrology. 2021; 9:115.","DOI":"10.1111\/andr.12886"},{"key":"54109","doi-asserted-by":"crossref","unstructured":"Puerta-Su\u00e1rez J, Giraldo M, Cadavid A, Cardona-Maya W. Infecciones bacterianas del tracto reproductivo masculino y su papel en la fertilidad. Rev Chil Obstet Ginecol 2014; 79:209.","DOI":"10.4067\/S0717-75262014000300010"},{"key":"54110","doi-asserted-by":"crossref","unstructured":"Galarzo S, Cano-Ch\u00e1ves A, Puerta Su\u00e1rez J, et al. Efecto de los factores solubles de Staphylococcus aureus, Staphylococcus capitis y Staphylococcus epidermidis sobre la fisiolog\u00eda esperm\u00e1tica. Aprobado para publicaci\u00f3n Rev Chil Obstet Ginecol 2015; 80:316-323.","DOI":"10.4067\/S0717-75262015000400006"},{"key":"54111","doi-asserted-by":"crossref","unstructured":"Cano-Ch\u00e1ves A, Galarzo-Pardo S, Puerta-Su\u00e1rez J, et al. Efecto de las bacterias uropat\u00f3genas y de los factores solubles de su metabolismo sobre la calidad esperm\u00e1tica: Escherichia coli y Enterococcus faecalis. Cl\u00ednica e Investigaci\u00f3n en Ginecolog\u00eda y Obstetricia. 2017;44:106-112.","DOI":"10.1016\/j.gine.2015.11.005"},{"key":"54112","doi-asserted-by":"crossref","unstructured":"Guerrero Hurtado L, Puerta Suarez J, Cardona Maya W. Papel de los espermatozoides en la transmisi\u00f3n de bacterias uropat\u00f3genas: Escherichia coli y Enterococcus faecalis. Cl\u00ednica e Investigaci\u00f3n en Ginecolog\u00eda y Obstetricia 2018:45:2-6.","DOI":"10.1016\/j.gine.2016.03.003"},{"key":"54113","doi-asserted-by":"crossref","unstructured":"Puerta Su\u00e1rez J, Cardona Maya W. Evaluaci\u00f3n in vitro del efecto de Neisseria gonorrhoeae y los factores solubles producto de su metabolismo sobre la calidad esperm\u00e1tica. 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