{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:27:46Z","timestamp":1790116066783,"version":"4.0.1"},"reference-count":30,"publisher":"PAGEPress Publications","issue":"1","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Ital Urol Androl"],"abstract":"<jats:p>Background: Spermatogenesis is worsened by reactive oxygen species (ROS) and antioxidants could reduce ROS induced sperm damage.Methods: This prospective cohort study evaluated the potential therapeutic effects of a combination of vitamins, minerals and antioxidants on the sperm quality parameters of infertile men with idiopathic low spermatogenesis. Seminal fluid analysis tests were performed before treatment, 3 and 6 months after treatment.Results: The treatment resulted in a significant improvement in the rate (%) of sperm motility from 16.95\u00b16.93 to 23.11\u00b18.87, after 3 months and reached 23.68\u00b18.73 after 6 months (p=0.0006) whereas a non-significant increase in sperm count (from 13.05\u00b18.1 to 15.79\u00b17.9 after 3 months and 15.26\u00b110.3 million\/ml after 6 months (p=0.1650). Morphology and agglutination showed little changes. A positive correlation between sperm count and motility was observed after 3 months of treatment (r = 0.594; p=0.007).Conclusions: The combination of vitamins and antioxidants improved the sperm motility significantly and to a lesser extent the sperm count, however, sperm morphology and agglutination remained relatively unchanged. Using antioxidants is safe and can improve semen parameters.<\/jats:p>","DOI":"10.4081\/aiua.2026.14685","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T13:09:05Z","timestamp":1772456945000},"source":"Crossref","is-referenced-by-count":2,"title":["Therapeutic supplementation with antioxidants and vitamins improved sperm motility among infertile men with idiopathic low spermatogenesis"],"prefix":"10.4081","volume":"98","author":[{"given":"Fadi","family":"Sawaqed","sequence":"first","affiliation":[{"name":"Section of Urology, Department of Special Surgery, Faculty of Medicine Mutah University, Karak"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Suoub","sequence":"additional","affiliation":[{"name":"Section of Urology, Department of Special Surgery, Faculty of Medicine Mutah University, Karak"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malik","family":"Ayyad","sequence":"additional","affiliation":[{"name":"Section of Urology, Department of Special Surgery, Faculty of Medicine Mutah University, Karak"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samer","family":"Al-Rawashdeh","sequence":"additional","affiliation":[{"name":"Section of Urology, Department of Special Surgery, Faculty of Medicine Mutah University, Karak"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef M.","family":"Al\u2011saraireh","sequence":"additional","affiliation":[{"name":"Section of Urology, Department of Special Surgery, Faculty of Medicine Mutah University, Karak"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Salem Hareedy","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Faculty of Medicine, Mutah University, Karak"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"216669","doi-asserted-by":"crossref","unstructured":"1. Ko EY, Siddiqi K, Brannigan RE, Sabanegh ES. Empirical medical therapy for idiopathic male infertility: a survey of the American Urological Association. J Urol. 2012; 187:973-8.","DOI":"10.1016\/j.juro.2011.10.137"},{"key":"216670","doi-asserted-by":"crossref","unstructured":"2. Kefer JC, Agarwal A, Sabanegh E. Role of antioxidants in the treatment of male infertility. Int J Urol. 2009; 16:449-57.","DOI":"10.1111\/j.1442-2042.2009.02280.x"},{"key":"216671","doi-asserted-by":"crossref","unstructured":"3. Agarwal A, Baskaran S, Parekh N, et al. Male infertility. Lancet 2021; 397:319-33.","DOI":"10.1016\/S0140-6736(20)32667-2"},{"key":"216672","unstructured":"4. Treatment of Infertility, Comhaire FH. Towards more objectivity in diagnosis and management of male infertility: results of a World Health Organization multicentre study. Oxford, Blackwell Scientific Publications; 1987."},{"key":"216673","doi-asserted-by":"crossref","unstructured":"5. Agarwal A, Parekh N, Panner Selvam MK, et al. Male oxidative stress infertility (MOSI): proposed terminology and clinical practice guidelines for management of idiopathic male infertility. World J Mens Health. 2019; 37:296-312.","DOI":"10.5534\/wjmh.190055"},{"key":"216674","doi-asserted-by":"crossref","unstructured":"6. Majzoub A, Agarwal A, Esteves SC. Antioxidants for elevated sperm DNA fragmentation: a mini review. Transl Androl Urol. 2017;6:S649-53.","DOI":"10.21037\/tau.2017.07.09"},{"key":"216675","doi-asserted-by":"crossref","unstructured":"7. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alexan J Med 2018; 54:287-93.","DOI":"10.1016\/j.ajme.2017.09.001"},{"key":"216676","doi-asserted-by":"crossref","unstructured":"8. Hussein TM, Eldabah N, Zayed HA, Genedy RM. Assessment of serum vitamin D level and seminal vitamin D receptor gene methylation in a sample of Egyptian men with idiopathic infertility. Andrologia. 2021; 53:e14172.","DOI":"10.1111\/and.14172"},{"key":"216677","unstructured":"9. WHO. Laboratory manual for the examination and processing of human semen, 6th ed. Available from: https:\/\/www.who.int\/publications\/i\/item\/9789240030787"},{"key":"216678","doi-asserted-by":"crossref","unstructured":"10. Ahmadi S, Bashiri R, Ghadiri-Anari A, Nadjarzadeh A. Antioxidant supplements and semen parameters: An evidence based review. Int J Reprod Biomed. 2016;14:729-36.","DOI":"10.29252\/ijrm.14.12.729"},{"key":"216679","doi-asserted-by":"crossref","unstructured":"11. Li K, Yang X, Wu T. The effect of antioxidants on sperm quality parameters and pregnancy rates for idiopathic male infertility: a network meta-analysis of randomized controlled trials. Front Endocrinol. 2022;13.","DOI":"10.3389\/fendo.2022.810242"},{"key":"216680","doi-asserted-by":"crossref","unstructured":"12. Dimitriadis F, Borgmann H, Struck JP, et al. Antioxidant supplementation on male fertility\u2014a systematic review. Antioxidants (Basel) 2023;12:836.","DOI":"10.3390\/antiox12040836"},{"key":"216681","doi-asserted-by":"crossref","unstructured":"13. Ibitoye BO, Fasasi AO, Imosemi IO, et al. The spermiogram and correlation of seminal fluid parameters in patient attending fertility centre in Lagos, South West Nigeria. Morphologie. 2023;107:100606.","DOI":"10.1016\/j.morpho.2023.05.004"},{"key":"216682","doi-asserted-by":"crossref","unstructured":"14. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2010; 4:118-26.","DOI":"10.4103\/0973-7847.70902"},{"key":"216683","doi-asserted-by":"crossref","unstructured":"15. Arafa M, Agarwal A, Majzoub A, et al. Efficacy of antioxidant supplementation on conventional and advanced sperm function tests in patients with idiopathic male infertility. Antioxidants (Basel). 2020;9:219.","DOI":"10.3390\/antiox9030219"},{"key":"216684","doi-asserted-by":"crossref","unstructured":"16. Ali M, Martinez M, Parekh N. Are antioxidants a viable treatment option for male infertility? Andrologia. 2021; 53:e13644.","DOI":"10.1111\/and.13644"},{"key":"216685","doi-asserted-by":"crossref","unstructured":"17. Menezo Y, Evenson D, Cohen M, Dale B. Effect of antioxidants on sperm genetic damage. Adv Exp Med Biol. 2014; 791:173-89.","DOI":"10.1007\/978-1-4614-7783-9_11"},{"key":"216686","doi-asserted-by":"crossref","unstructured":"18. Cardoso JP, Cocuzza M, Elterman D. Optimizing male fertility: oxidative stress and the use of antioxidants. World J Urol. 2019;37:1029-34.","DOI":"10.1007\/s00345-019-02656-3"},{"key":"216687","doi-asserted-by":"crossref","unstructured":"19. Kumar R, Gautam G, Gupta NP. Drug therapy for idiopathic male infertility: rationale versus evidence. J Urol. 2006; 176:1307-12.","DOI":"10.1016\/j.juro.2006.06.006"},{"key":"216688","doi-asserted-by":"crossref","unstructured":"20. Kumar N, Singh AK. Reactive oxygen species in seminal plasma as a cause of male infertility. J Gynecol Obstet Hum Reprod. 2018;47:565-72.","DOI":"10.1016\/j.jogoh.2018.06.008"},{"key":"216689","doi-asserted-by":"crossref","unstructured":"21. Benedetti S, Tagliamonte MC, Catalani S, et al. Differences in blood and semen oxidative status in fertile and infertile men, and their relationship with sperm quality. Reprod Biomed Online 2012;25:300-6.","DOI":"10.1016\/j.rbmo.2012.05.011"},{"key":"216690","doi-asserted-by":"crossref","unstructured":"22. Boushaba S, Helis Y, Lebaal R, et al. The relationship of sperm DNA integrity with serum vitamin levels (folate and cobalamin) and food consumption in infertile men. Clin Exp Reprod Med. 2023;50:53-62.","DOI":"10.5653\/cerm.2022.05736"},{"key":"216691","doi-asserted-by":"crossref","unstructured":"23. Banihani SA. Vitamin B12 and semen quality. Biomolecules 2017; 7:42.","DOI":"10.3390\/biom7020042"},{"key":"216692","doi-asserted-by":"crossref","unstructured":"24. Hosseinabadi F, Jenabi M, Ghafarizadeh AA, Yazdanikhah S. The effect of vitamin B12 supplement on post-thaw motility, viability and DNA damage of human sperm. Andrologia. 2020; 52:e13877.","DOI":"10.1111\/and.13877"},{"key":"216693","doi-asserted-by":"crossref","unstructured":"25. Kumar N, Jadhao AG, Yadav RR. Correlation between serum vitamin B12, vitamin D, and suboptimal semen parameters in male infertility: A hospital-based cross-sectional study. J Family Med Prim Care. 2024; 13:5171-6.","DOI":"10.4103\/jfmpc.jfmpc_727_24"},{"key":"216694","doi-asserted-by":"crossref","unstructured":"26. Arab A, Hadi A, Moosavian SP, et al. The association between serum vitamin D, fertility and semen quality: A systematic review and meta-analysis. Int J Surg 2019;71:101-9.","DOI":"10.1016\/j.ijsu.2019.09.025"},{"key":"216695","doi-asserted-by":"crossref","unstructured":"27. Banks N, Sun F, Krawetz SA, et al. Male vitamin D status and male factor infertility. Fertil Steril 2021; 116:973-9.","DOI":"10.1016\/j.fertnstert.2021.06.035"},{"key":"216696","doi-asserted-by":"crossref","unstructured":"28. Kumari S, Singh K, Kumari S, et al. Association of vitamin D and reproductive hormones with semen parameters in infertile men. Cureus. 2021; 13:e14511.","DOI":"10.7759\/cureus.14511"},{"key":"216697","doi-asserted-by":"crossref","unstructured":"29. Maghsoumi-Norouzabad L, Labibzadeh M, Zare Javid A, et al. The association of vitamin D, semen parameters, and reproductive hormones with male infertility: A cross-sectional study. Int J Reprod Biomed. 2022; 20:331-8.","DOI":"10.18502\/ijrm.v20i4.10905"},{"key":"216698","doi-asserted-by":"crossref","unstructured":"30. Kaltsas A. Oxidative stress and male infertility: the protective role of antioxidants. 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