{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:44:04Z","timestamp":1782740644920,"version":"3.54.5"},"reference-count":11,"publisher":"PAGEPress Publications","issue":"2","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arch Ital Urol Androl"],"abstract":"<jats:p>Introduction and objectives: Automated urinalysis offers rapid and efficient evaluation of urine sediment; however, most systems do not routinely report epithelial cell atypia. This study aimed to evaluate the clinical relevance of the atypical cell (Atyp.C) parameter generated by the Sysmex UF-4000 analyzer as an early indicator of suspected urothelial carcinoma.\nMaterials and methods: A prospective study was conducted using the UF-4000 analyzer to detect Atyp.C in urine specimens from high-risk patients. Atyp.C results were collected simultaneously for all samples. Specimens with positive Atyp.C findings underwent urinary tract cytology, which was independently reviewed by board-certified cytopathologists and categorized into four diagnostic groups. Statistical analyses were performed using IBM SPSS version 21.\nResults: Among 332 specimens, 20 samples (6.02%) showed Atyp.C values &gt; 0.0\/\u03bcL. The mean Atyp.C value was 0.6\/\u03bcL (95% CI 0.064-1.135) in males and 1.20\/\u03bcL (95% CI 0.608- 1.791) in females, with no significant difference between sexes (p = 0.2549). Of the 20 Atyp.C-positive samples, 5 (25%) demonstrated abnormal cytology, including 2 cases of atypical urothelial cells, 1 case suspicious for high-grade malignancy, and 2 cases of confirmed high-grade urothelial carcinoma. Atyp.C values were significantly higher in abnormal compared with benign cytology (p &lt; 0.01).\nConclusions: The Atyp.C parameter may have potential as an adjunctive screening marker for urothelial abnormalities; however, further validation studies are required.<\/jats:p>","DOI":"10.4081\/aiua.2026.15595","type":"journal-article","created":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T13:36:07Z","timestamp":1782480967000},"source":"Crossref","is-referenced-by-count":0,"title":["Atypical urothelial cells investigation with automated urinalysis and expert review of microscopic sediment as early parameter for suspected bladder cancer patients"],"prefix":"10.4081","volume":"98","author":[{"given":"Dwi Evan Prima Putra","family":"Noviardi","sequence":"first","affiliation":[{"name":"Department of Urology, Faculty of Medicine, Universitas Brawijaya, Saiful Anwar Hospital, Malang"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurnia Penta","family":"Seputra","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, Universitas Brawijaya, Saiful Anwar Hospital, Malang"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Besut","family":"Daryanto","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, Universitas Brawijaya, Saiful Anwar Hospital, Malang"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fauzan Kurniawan","family":"Dhani","sequence":"additional","affiliation":[{"name":"Department of Urology, Faculty of Medicine, Universitas Brawijaya, Saiful Anwar Hospital, Malang"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hani","family":"Susianti","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Saiful Anwar Hospital, Malang"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenty Wantri","family":"Anita","sequence":"additional","affiliation":[{"name":"Department of Pathology Anatomy, Faculty of Medicine, Universitas Brawijaya, Saiful Anwar Hospital, Malang"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2026,6,26]]},"reference":[{"key":"237824","doi-asserted-by":"crossref","unstructured":"1. Oyaert M, Delanghe J. Progress in Automated Urinalysis. Ann Lab Med. 2019; 39:15-22.","DOI":"10.3343\/alm.2019.39.1.15"},{"key":"237825","doi-asserted-by":"crossref","unstructured":"2. Miyazaki J, Nishiyama H. Epidemiology of urothelial carcinoma. Int J Urol 2017; 24:730-734.","DOI":"10.1111\/iju.13376"},{"key":"237826","doi-asserted-by":"crossref","unstructured":"3. Fogazzi GB, Pallotti F, Garigali G. Atypical\/malignant urothelial cells in routine urinary sediment: worth knowing and reporting. Clin Chim Acta. 2015; 439:107-11.","DOI":"10.1016\/j.cca.2014.10.021"},{"key":"237827","doi-asserted-by":"crossref","unstructured":"4. Aydin O. Atypical cells parameter in Sysmex UN automated urine analyzer: feedback from the field. Diagn Pathol. 2021; 16:9.","DOI":"10.1186\/s13000-021-01068-5"},{"key":"237828","doi-asserted-by":"crossref","unstructured":"5. Barnes PW, McFadden SL, Machin SJ, Simson E; international consensus group for hematology. The international consensus group for hematology review: suggested criteria for action following automated CBC and WBC differential analysis. Lab Hematol. 2005; 11:83-90.","DOI":"10.1532\/LH96.05019"},{"key":"237829","doi-asserted-by":"crossref","unstructured":"6. Aydin O. Atypical cells parameter in an automated urine analyzer: Does it have a future? Anal Biochem. 2020; 600:113763.","DOI":"10.1016\/j.ab.2020.113763"},{"key":"237830","doi-asserted-by":"crossref","unstructured":"7. Shukuya K, Morita Y, Hisasue T, et al. Comparison of the clinical performance of the Atyp.C parameter of the UF-5000 fully automated urine particle analyzer with that of microscopic urine sediment analysis. Pract Lab Med. 2023; 36:e00328.","DOI":"10.1016\/j.plabm.2023.e00328"},{"key":"237831","doi-asserted-by":"crossref","unstructured":"8. Anderlini R, Manieri G, Lucchi C, et al. Automated urinalysis with expert review for incidental identification of atypical urothelial cells: An anticipated bladder carcinoma diagnosis. Clin Chim Acta. 2015; 451(Pt B):252-256.","DOI":"10.1016\/j.cca.2015.10.005"},{"key":"237832","doi-asserted-by":"crossref","unstructured":"9. Barkan GA, Wojcik EM, Nayar R, et al. The Paris System for Reporting Urinary Cytology: The Quest to Develop a Standardized Terminology. Adv Anat Pathol. 2016; 23:193-201.","DOI":"10.1097\/PAP.0000000000000118"},{"key":"237833","doi-asserted-by":"crossref","unstructured":"10. Xing J, Reynolds JP. Diagnostic Advances in Urine Cytology. Surg Pathol Clin. 2018; 11:601-610.","DOI":"10.1016\/j.path.2018.06.001"},{"key":"237834","doi-asserted-by":"crossref","unstructured":"11. Willner J, Matloob A, Colanta A, Khader SN. Educational Case: Urothelial Carcinoma: An Overview of Pathologic Diagnosis. Acad Pathol. 2020; 7:2374289520958172.","DOI":"10.1177\/2374289520958172"}],"container-title":["Archivio Italiano di Urologia e Andrologia"],"original-title":[],"link":[{"URL":"https:\/\/www.pagepressjournals.org\/aiua\/article\/download\/15595\/14231","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.pagepressjournals.org\/aiua\/article\/download\/15595\/14231","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T12:45:42Z","timestamp":1782737142000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.pagepressjournals.org\/aiua\/article\/view\/15595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,26]]},"references-count":11,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,6,29]]}},"URL":"https:\/\/doi.org\/10.4081\/aiua.2026.15595","relation":{},"ISSN":["2282-4197","1124-3562"],"issn-type":[{"value":"2282-4197","type":"electronic"},{"value":"1124-3562","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,26]]}}}