{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T20:23:33Z","timestamp":1767990213600,"version":"3.49.0"},"reference-count":0,"publisher":"PAGEPress Publications","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":["www.ejh.it"],"crossmark-restriction":true},"short-container-title":["Eur J Histochem"],"abstract":"<jats:p>Dentinogenesis imperfecta determines structural alterations of the collagen structure still not completely elucidated. Immunohistochemical analysis was used to assay Type I and VI collagen, various non-collagenous proteins distribution in human primary teeth from healthy patients or from patients affected by type I dentinogenesis imperfecta (DGI-I) associated with osteogenesis imperfecta (OI). In sound primary teeth, an organized well-known ordered pattern of the type I collagen fibrils was found, whereas atypical and disorganized fibrillar structures were observed in dentin of DGI-I affected patients. Expression of type I collagen was observed in both normal and affected primary teeth, although normal dentin stained more uniformly than DGI-I affected dentin. Reactivity of type VI collagen was significantly lower in normal teeth than in dentin from DGI-I affected patients (P&lt;0.05). Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences, being DMP1 generally more abundantly expressed. Immunolabeling for chondroitin sulfate (CS) and biglycan (BGN) was weaker in dentin from DGI-I-affected patients compared to normal dentin, this decrease being significant only for CS. This study shows ultrastructural alterations in dentin obtained from patients affected by DGI-I, supported by immunocytochemical assays of different collagenous and non-collagenous proteins.<\/jats:p>","DOI":"10.4081\/ejh.2014.2405","type":"journal-article","created":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T13:54:24Z","timestamp":1417442064000},"update-policy":"https:\/\/doi.org\/10.4081\/ejh.crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Immunocytochemical detection of dentin matrix proteins in primary teeth from patients with dentinogenesis imperfecta associated with osteogenesis imperfecta"],"prefix":"10.4081","author":[{"given":"G.","family":"Orsini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Majorana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Mazzoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Putignano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Falconi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Polimeni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Breschi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2549","published-online":{"date-parts":[[2014,12,1]]},"container-title":["European Journal of Histochemistry"],"original-title":[],"link":[{"URL":"https:\/\/www.ejh.it\/index.php\/ejh\/article\/download\/2405\/2312","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ejh.it\/index.php\/ejh\/article\/download\/2405\/2386","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ejh.it\/index.php\/ejh\/article\/download\/2405\/2312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,3]],"date-time":"2020-07-03T08:20:15Z","timestamp":1593764415000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejh.it\/index.php\/ejh\/article\/view\/2405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.4081\/ejh.2014.2405","relation":{},"ISSN":["2038-8306","1121-760X"],"issn-type":[{"value":"2038-8306","type":"electronic"},{"value":"1121-760X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}