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The aim of this study was to explore the role of HMGB1 in inflammation and catabolism of temporomandibular joint osteoarthritis (TMJOA) and whether inhibition of HMGB1 affects TMJOA. Human synovial fibroblasts were incubated with HMGB1, the expression of pro-inflammatory cytokines and catabolic mediators were measured by Western blot and ELISA. NF-\u03baB signaling pathway involvement was studied by the NF-\u03baB inhibitor and detected by Western blotting and immunofluorescence staining. TMJOA was induced by an injection of Complete Freund\u2019s adjuvant (CFA) into anterosuperior compartment of rat\u2019s joint. An anti-HMGB1 antibody was used to assess the effect to HMGB1 in the synovium and cartilage of the CFA-induced TMJOA rats by H&amp;E, Safranin O, Masson trichrome staining, immunohistochemistry and immunofluorescence. HMGB1 markedly increased the production of MMP13, ADAMTS5, IL-1\u03b2 and IL-6 through activating NF-\u03baB signaling pathway in human synovial fibroblasts. In vivo, application of the HMGB1 neutralizing antibody effectively ameliorated the detrimental extent of TMJOA. Furthermore, the HMGB1 neutralizing antibody reduced the expression of NF-\u03baB, pro-inflammatory cytokines and catabolic mediators in the synovium and cartilage of CFA-induced TMJOA rats. HMGB1 inhibition alleviates TMJOA by reducing synovial inflammation and cartilage catabolism possibly through suppressing the NF-\u03baB signaling pathway and may become a therapeutic method against TMJOA.<\/jats:p>","DOI":"10.4081\/ejh.2022.3357","type":"journal-article","created":{"date-parts":[[2022,6,23]],"date-time":"2022-06-23T11:26:57Z","timestamp":1655983617000},"source":"Crossref","is-referenced-by-count":12,"title":["Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis &lt;em&gt;via&lt;\/em&gt; NF-\u03baB signaling pathway"],"prefix":"10.4081","volume":"66","author":[{"given":"Yan Yan","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya Ping","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Ke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Long","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2549","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"8634","doi-asserted-by":"crossref","unstructured":"Wang XD, Zhang JN, Gan YH, Zhou YH. 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