{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T11:40:06Z","timestamp":1747309206510,"version":"3.40.5"},"reference-count":17,"publisher":"PAGEPress Publications","issue":"2","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Histochem"],"abstract":"<jats:p>This study investigates the expression and biological role of caudal homologous transcription factor 2 (CDX2) in duodenal carcinoma. Paraffin-embedded samples from 40 duodenal carcinoma cases were analyzed using immunohistochemistry on a tissue microarray to assess CDX2 expression and its prognostic significance. CDX2 overexpression plasmids and CDX2-siRNA were transfected into colon and duodenal cancer cells. Transfection efficiency was confirmed by RT-PCR and Western blotting. Cell proliferation was assessed using CCK8 assay, migration via scratch assay, and cell cycle and apoptosis by flow cytometry. CDX2 staining was primarily nuclear, with a positive rate of 65% in duodenal carcinoma tissues, significantly lower than in adjacent non-tumor tissues (p&lt;0.05). CDX2-positive patients had better prognoses than negative patients (p&lt;0.05). Reduced CDX2 expression significantly enhanced the proliferation of CaCO2 and HuTu-80 cells (p&lt;0.001), whereas CDX2 overexpression suppressed proliferation (p&lt;0.001). CDX2 knockdown increased migration, while its overexpression reduced migration (p&lt;0.05). CDX2 overexpression led to a significant increase in G0\/G1 phase cells and a decrease in S phase cells (p&lt;0.05), whereas knockdown reduced G0\/G1 phase cells and increased S and G2\/M phase cells (p&lt;0.05). Apoptosis was significantly increased following CDX2 overexpression (p&lt;0.001) and decreased after CDX2 knockdown (p&lt;0.001). CDX2 expression is downregulated or lost in duodenal carcinoma, acting as a tumor suppressor. CDX2 may serve as a crucial biomarker for predicting the onset, progression, and treatment of duodenal carcinoma.<\/jats:p>","DOI":"10.4081\/ejh.2025.4203","type":"journal-article","created":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T08:22:54Z","timestamp":1745482974000},"source":"Crossref","is-referenced-by-count":0,"title":["Effect of CDX2 on proliferation, invasion, migration, and apoptosis of duodenal cancer cells"],"prefix":"10.4081","volume":"69","author":[{"given":"Jun","family":"Pan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhe","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengxuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yitian","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyuan","family":"Chu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2549","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"40553","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21590"},{"key":"40554","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-018-6567-6"},{"key":"40555","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2018.1433321"},{"key":"40556","doi-asserted-by":"publisher","DOI":"10.1177\/0300060518819620"},{"key":"40557","doi-asserted-by":"publisher","DOI":"10.1111\/his.14830"},{"key":"40558","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2023.102643"},{"key":"40559","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12982"},{"key":"40560","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-020-01148-1"},{"key":"40561","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25168673"},{"key":"40562","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12347"},{"key":"40563","doi-asserted-by":"publisher","DOI":"10.1007\/s12029-019-00314-w"},{"key":"40564","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4902-8"},{"key":"40565","doi-asserted-by":"publisher","DOI":"10.4103\/tcmj.tcmj_49_20"},{"key":"40566","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/9025668"},{"key":"40567","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v28.i26.3027"},{"key":"40568","doi-asserted-by":"publisher","DOI":"10.3390\/cells9092125"},{"key":"40569","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1263-9"}],"container-title":["European Journal of Histochemistry"],"original-title":[],"link":[{"URL":"https:\/\/www.ejh.it\/ejh\/article\/download\/4203\/3565","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ejh.it\/ejh\/article\/download\/4203\/3573","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.ejh.it\/ejh\/article\/download\/4203\/3565","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T10:59:18Z","timestamp":1747306758000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ejh.it\/ejh\/article\/view\/4203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":17,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,4,7]]}},"URL":"https:\/\/doi.org\/10.4081\/ejh.2025.4203","relation":{},"ISSN":["2038-8306","1121-760X"],"issn-type":[{"type":"electronic","value":"2038-8306"},{"type":"print","value":"1121-760X"}],"subject":[],"published":{"date-parts":[[2025,4,24]]}}}