{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T14:10:53Z","timestamp":1791727853283,"version":"4.3.4"},"reference-count":30,"publisher":"PAGEPress Publications","issue":"4","license":[{"start":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T00:00:00Z","timestamp":1758585600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Histochem"],"abstract":"<jats:p>Cervical cancer is a serious gynecological malignancy, and the specific mechanisms of miR-129-5p remain unclear. This study aims to investigate the mechanism by which miR-129-5p regulates the high mobility group box 1 (HMGB1\/receptor for advanced glycation end-products (RAGE) axis to inhibit pyroptosis and ameliorate cervical epithelial cell deterioration. Using RT-qPCR and Western blotting, we detected significantly downregulated miR-129-5p and upregulated HMGB1 in cervical cancer cells. To establish a deterioration model, we stimulated cervical epithelial cells with lipopolysaccharide (LPS). Further results revealed that miR-129-5p overexpression markedly reduced HMGB1 expression, suppressed RAGE activation, and decreased pyroptosis executer GSDMD-N production. Additionally, we conducted miR-129-5p overexpression and knockdown experiments to verify its regulatory effects on the HMGB1\/RAGE axis and downstream pathways. Caspase-1 activity assays confirmed reduced pyroptosis upon miR-129-5p overexpression. Cell viability and proliferation were assessed using EdU incorporation assays and colony formation experiments. Our data demonstrated significant downregulation of miR-129-5p in cervical cancer cells. Overexpression of miR-129-5p substantially reduced HMGB1 expression and inhibited RAGE activation, thereby decreasing production of the pyroptosis executer GSDMD-N. LPS stimulation potently activated the HMGB1\/RAGE axis and induced pyroptosis, while miR-129-5p overexpression inhibited these processes and ameliorated in vitro cervical epithelial cell deterioration. Cells overexpressing miR-129-5p exhibited attenuated caspase-1 activity with enhanced survival and proliferation following LPS treatment. Collectively, these in vitro findings indicate that miR-129-5p suppresses HMGB1\/RAGE-mediated pyroptosis and cellular deterioration and also provide new mechanistic insights for cervical cancer therapeutics.<\/jats:p>","DOI":"10.4081\/ejh.2025.4238","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T12:03:04Z","timestamp":1758628984000},"source":"Crossref","is-referenced-by-count":3,"title":["miR-129-5p regulates HMGB1\/RAGE axis to inhibit pyroptosis and ameliorate cervical epithelial cell deterioration"],"prefix":"10.4081","volume":"69","author":[{"given":"Huafang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilidaer","family":"Sidike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longge","family":"Suo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huerxidan","family":"Niyazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2025,9,23]]},"reference":[{"key":"43593","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32470-X"},{"key":"43594","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2021.03.006"},{"key":"43595","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2022.110354"},{"key":"43596","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222212165"},{"key":"43597","doi-asserted-by":"publisher","DOI":"10.1136\/jmedgenet-2018-105387"},{"key":"43598","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.109995"},{"key":"43599","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021792"},{"key":"43600","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00150"},{"key":"43601","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2018.1491497"},{"key":"43602","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2018.1491497"},{"key":"43603","unstructured":"11. Feng J, Guo J, Wang JP, Chai BF. MiR-129-5p inhibits proliferation of gastric cancer cells through targeted inhibition on HMGB1 expression. 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