{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T18:44:50Z","timestamp":1790361890716,"version":"4.1.0"},"reference-count":50,"publisher":"PAGEPress Publications","issue":"2","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Histochem"],"abstract":"<jats:p>Astragaloside IV (AS IV) inhibits the malignant phenotype of nasopharyngeal carcinoma (NPC), but whether its mechanism involves the regulation of immune checkpoint programmed cell death-ligand 1 (PD-L1) is not clear. Human NPC cells were treated with AS IV. The effects of AS IV on PD-L1 expression were assessed using RT-qPCR and Western blot. SATB2\/Wnt\/\u03b2-catenin signaling axis regulation was analyzed by siRNA interference, plasmid overexpression and Wnt pathway inhibitor DKK-1. T cell killing activity and tumor malignant phenotype were evaluated by LDH release, ELISA, flow cytometry and Transwell experiments. huHSC-NCG tumor-bearing mice were established to detect tumor growth, immune cell infiltration and related protein expression. AS IV dose-dependently inhibited PD-L1 expressions within NPC cells, and enhanced the activation and killing function of CD8+ T cells. Mechanism studies have shown that AS IV significantly lowered the expression of SATB2, thereby inhibiting Wnt\/\u03b2-catenin axis and c-MYC and Axin2 expressions, and ultimately reducing PD-L1 levels. Overexpression of SATB2 reversed AS IV's suppression of this signaling and PD-L1. Animal experiments confirmed that AS IV effectively inhibited tumor growth, enhanced CD8+ T cell infiltration and activity within tumor tissues, and down-regulated the SATB2\/\u03b2-catenin\/PD-L1 signal axis. AS IV inhibited PD-L1 expression in NPC via targeting the SATB2\/Wnt\/\u03b2-catenin axis, thereby activating CD8+ T cells, amplifying immunological responses, and ultimately inhibiting NPC growth.\n\u00a0\nGraphical Abstract<\/jats:p>","DOI":"10.4081\/ejh.2026.4540","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T14:23:53Z","timestamp":1779978233000},"source":"Crossref","is-referenced-by-count":1,"title":["Astragaloside IV inhibits nasopharyngeal carcinoma progression by inhibiting SATB2\/Wnt\/PD-L1 pathway and enhancing the killing activity of T cells"],"prefix":"10.4081","volume":"70","author":[{"given":"Yinping","family":"Zeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"51341","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2023.5545"},{"key":"51342","doi-asserted-by":"publisher","DOI":"10.1097\/SCS.0000000000008765"},{"key":"51343","doi-asserted-by":"publisher","DOI":"10.1111\/coa.13917"},{"key":"51344","doi-asserted-by":"publisher","DOI":"10.62382\/jcbt.v1i1.12"},{"key":"51345","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2021.105182"},{"key":"51346","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-022-00874-5"},{"key":"51347","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-022-00873-6"},{"key":"51348","doi-asserted-by":"publisher","DOI":"10.4251\/wjgo.v13.i11.1725"},{"key":"51349","doi-asserted-by":"publisher","DOI":"10.4103\/ijc.IJC_459_19"},{"key":"51350","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230449"},{"key":"51351","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112523"},{"key":"51352","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.10.034"},{"key":"51353","doi-asserted-by":"publisher","DOI":"10.63623\/e33gn241"},{"key":"51354","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-023-01044-w"},{"key":"51355","doi-asserted-by":"publisher","DOI":"10.1186\/s12896-025-01001-4"},{"key":"51356","doi-asserted-by":"publisher","DOI":"10.1186\/s13062-025-00645-8"},{"key":"51357","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-025-01072-7"},{"key":"51358","doi-asserted-by":"publisher","DOI":"10.1007\/s12022-024-09833-0"},{"key":"51359","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbo.2023.100491"},{"key":"51360","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2025.1543426"},{"key":"51361","unstructured":"21. 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