{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T21:40:11Z","timestamp":1717537211501},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2014,6,26]],"date-time":"2014-06-26T00:00:00Z","timestamp":1403740800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematology Reports"],"abstract":"<jats:p>Hematogones are precursors of B-lymphocytes detected in small numbers in the bone marrow. Flow cytometry is the most useful tool to identify hematogones and, so far, 4-color methods have been published. In addition, flow cytometry is used in the diagnosis and follow-up of lymphomas. We developed a flow cytometric 7-color method to enumerate hematogones and to assess B-lymphocyte clonality for routine purposes. We evaluated 171 cases of B-cell non-Hodgkin lymphomas, either at diagnosis or in the course of follow-up. By our diagnostic method, which was carried out by the combination K\/\u03bb\/CD20\/CD19\/CD10\/CD45\/CD5, we were able to detect hematogones in 97.6% of samples and to distinguish normal B-lymphocytes, neoplastic lymphocytes and hematogones in a single step. The percentage of hematogones showed a significant inverse correlation with the degree of neoplastic infiltration and, when bone marrow samples not involved by disease were taken into consideration, resulted higher in patients during follow-up than in patients evaluated at diagnosis.<\/jats:p>","DOI":"10.4081\/hr.2014.5381","type":"journal-article","created":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T09:12:16Z","timestamp":1415351536000},"page":"5381","source":"Crossref","is-referenced-by-count":4,"title":["Multiparameter Flow Cytometry to Detect Hematogones and to Assess B-Lymphocyte Clonality in Bone Marrow Samples from Patients with Non-Hodgkin Lymphomas"],"prefix":"10.3390","volume":"6","author":[{"given":"Giovanni","family":"Carulli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Ottaviano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Guerri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Giuntini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Sammuri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugenio M.","family":"Ciancia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Azzar\u00e0","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2014,6,26]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1034\/j.1600-065X.2002.18904.x","article-title":"A developing picture of lymphopoiesis in bone marrow","volume":"189","author":"Hirose","year":"2002","journal-title":"Immunol Rev"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/cmi.2011.47","article-title":"Bone marrow and the control of immunity","volume":"9","author":"Zhao","year":"2012","journal-title":"Cell Mol Immunol"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1309\/LXU4-Q7Q9-3YAB-4QE0","article-title":"Benign hematogone-rich lymphoid proliferations can be distinguished from B-lineage acute lymphoblastic leukemia by integration of morphology, immunophenotype, adhesion molecule expression, and architectural features","volume":"114","author":"Rimsza","year":"2000","journal-title":"Am J Clin Pathol"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1016\/j.bbmt.2011.11.015","article-title":"Impact of bone marrow hematogones on umbilical cord blood transplantation outcomes in patients with acute myeloid leukemia","volume":"18","author":"Honebrink","year":"2012","journal-title":"Biol Blood Marrow Transplant"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"10","DOI":"10.3109\/10428190903370346","article-title":"Hematogones: a review and update","volume":"51","author":"Sevilla","year":"2010","journal-title":"Leuk Lymphoma"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"1404","DOI":"10.1016\/j.leukres.2013.07.024","article-title":"Hematogones: an overview","volume":"37","author":"Chantepie","year":"2013","journal-title":"Leuk Res"},{"key":"ref7","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1182\/asheducation-2009.1.523","article-title":"The 2008 WHO classification of lymphomas: implications for clinical practice and translational research","author":"Jaffe","year":"2009","journal-title":"Hematology Am Soc Hematol Educ Program"},{"key":"ref8","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1111\/j.1365-2559.2010.03703.x","article-title":"Flow cytometry and its use in the diagnosis and management of mature lymphoid malignancies","volume":"58","author":"De Tute","year":"2011","journal-title":"Histopathology"},{"key":"ref9","first-page":"47","article-title":"Diagnosis and classification of B-cell non-Hodgkin lymphomas. 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