{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T16:08:14Z","timestamp":1781712494714,"version":"3.54.5"},"reference-count":24,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T00:00:00Z","timestamp":1458259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hematology Reports"],"abstract":"<jats:p>Autologous hematopoietic stem cell transplantation (auto-HSCT) provides hematopoietic support after high-dose chemotherapy and is the standard of care for patients with multiple myeloma (MM), chemo sensitive relapsed high or intermediate grade non-Hodgkin\u2019s lymphoma (NHL) and Hodgkin\u2019s lymphoma (HL). However, yields of hematopoietic stem cells vary greatly between patients, and the optimal strategy to mobilize hematopoietic stem cells into peripheral blood for collection has not been defined yet. We investigated the efficacy and safety of chemo mobilization with an intermediate dose etoposide (VP-16; 200 mg\/m2 on days 1\u20133) and granulocyte-colony stimulating factor (G-CSF)(5 \u03bcg\/kg twice daily from day 4 through the final day of collection). We reviewed our institutional experience with 91 patients (71 MM, 12 HL, 8 NHL) mobilized with this regimen. VP-16 + G-CSF resulted in successful mobilization in 95.55% of the patients (on one patient stem cell collection with plerixafor was applied), including 76 patients (83.52%) whose stem cells were collected successfully in a single day. Collection was managed between min. D8 and max. D17. Patient age, gender, exposure to previous irradiation and chemotherapy, previous mobilization attempts, and disease characteristics were not considered during selection. Adverse effects of the regimen included supportive transfusions and fevers requiring hospitalization or intravenous antibiotics. VP-16 and GCSF appears to be a safe and effective mobilization regimen for patients with multiple myeloma, non-Hodgkin\u2019s lymphoma and Hodgkin\u2019s lymphoma undergoing autologous stem cell transplantation, producing excellent stem cell yield with the majority of patients requiring 1 day of apheresis.<\/jats:p>","DOI":"10.4081\/hr.2016.6319","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T14:58:04Z","timestamp":1458313084000},"page":"6319","source":"Crossref","is-referenced-by-count":16,"title":["The High Effect of Chemomobilization with High-Dose Etopside + Granulocyte-Colony Stimulating Factor in Autologous Hematopoietic Peripheral Blood Stem Cell Transplantation: A Single Center Experience"],"prefix":"10.3390","volume":"8","author":[{"given":"\u0218ebnem","family":"Izmir Gu\u0308ner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Teoman","family":"Yanmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"Selvi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cigdem","family":"Usul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2016,3,18]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1182\/blood.V82.12.3770.3770","article-title":"Peripheral blood progenitor cell transplantation in lymphoma and leukemia using a single apheresis","volume":"82","author":"Pettengell","year":"1993","journal-title":"Blood"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"3961","DOI":"10.1182\/blood.V86.10.3961.bloodjournal86103961","article-title":"An analysis of engraftment kinetics as a function of the CD34 content of peripheral blood progenitor cell collections in 692 patients after the administration of myeloablative chemotherapy","volume":"86","author":"Weaver","year":"1995","journal-title":"Blood"},{"key":"ref3","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1182\/blood.V85.2.588.588","article-title":"Peripheral blood stem cell transplants for multiple myeloma: identification of favorable variables for rapid engraftment in 225 patients","volume":"85","author":"Tricot","year":"1995","journal-title":"Blood"},{"key":"ref4","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1056\/NEJMoa022340","article-title":"High-dose chemotherapy with hematopoietic stem cell rescue for multiple myeloma","volume":"348","author":"Child","year":"2003","journal-title":"N Engl J Med"},{"key":"ref5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/sj.bmt.1705194","article-title":"Cure of multiple myeloma - more hype, less reality","volume":"37","author":"Hari","year":"2006","journal-title":"Bone Marrow Transplant"},{"key":"ref6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1056\/NEJM199607113350204","article-title":"A prospective, randomized trial of autologous bone marrow transplantation and chemotherapy in multiple myeloma. 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Netw"},{"key":"ref13","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1080\/10245332.1999.11746443","article-title":"Bone marrow transplantation: prognostic factors of peripheral blood stem cell mobilization with cyclophosphamide and filgrastim (r-metHuG-CSF): the CD34+ cell dose positively affects the time to hematopoietic recovery and supportive requirements after high-dose chemotherapy","volume":"4","author":"Sola","year":"1999","journal-title":"Hematology"},{"key":"ref14","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1056\/NEJM199512073332305","article-title":"Autologous bone marrow transplantation compared with salvage chemotherapy in relapses of chemotherapy-sensitive non-Hodgkin\u2019s lymphoma","volume":"333","author":"Philip","year":"1995","journal-title":"N Engl J Med"},{"key":"ref15","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1111\/j.1365-2141.2004.04837.x","article-title":"Favourable results with a single autologous stem cell transplant 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