{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:10:06Z","timestamp":1789715406177,"version":"4.0.1"},"reference-count":0,"publisher":"PAGEPress Publications","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ital J Food Safety"],"abstract":"<jats:p>Healthy pigs carrying pathogenic to human &lt;em&gt;Yersinia enterocolitica&lt;\/em&gt; strains are the main source of entry into slaughterhouse, where cross-contamination of carcasses can happen. The aim of this work was to determine &lt;em&gt;Y. enterocolitica&lt;\/em&gt; prevalence in slaughtered pigs, investigating the presence of carriers in relation to carcass contamination. A total of 132 pig samples (tonsils, mesenteric lymph nodes, colon content, carcass surface) were collected from 4 Sardinian slaughterhouses. All the samples were examined by the ISO 10273:2003 method, and the prevalence was also determined by direct plating on CIN Agar. Moreover, to detect the &lt;em&gt;ail&lt;\/em&gt; positive &lt;em&gt;Y. enterocolitica&lt;\/em&gt; strains in enrichment broths and isolates a real-time polymerase chain reaction (PCR) was applied. &lt;em&gt;Y. enterocolitica&lt;\/em&gt; prevalence was 19% with direct plating and 12% with enrichment methods. Carcass surfaces and tonsils prevalence was 5.30% by direct plating, and 5.3% and 2.2%, respectively, by enrichment method. Tonsil samples showed an average contamination level of 3.2\u00d7103 CFU\/g, while the mean value on carcass was 8.7\u00d7102 CFU\/g. An overall prevalence of 9.8% of &lt;em&gt;ail&lt;\/em&gt; positive &lt;em&gt;Y. enterocolitica&lt;\/em&gt; broths was detected by RT-PCR, that found a higher prevalence in tonsils (7.5%) with respect to cultural methods, confirming the greater sensitivity of this technique when applied for tonsils and faeces samples. The results show a relatively low pathogenic &lt;em&gt;Y. enterocolitica&lt;\/em&gt; prevalence in pigs slaughtered in Sardinia. Good hygiene measures should be applied at slaughterhouse in order to prevent the entry of carriers and control carcass contamination.<\/jats:p>","DOI":"10.4081\/ijfs.2015.4579","type":"journal-article","created":{"date-parts":[[2015,6,15]],"date-time":"2015-06-15T08:25:54Z","timestamp":1434356754000},"source":"Crossref","is-referenced-by-count":4,"title":["Detection of pathogenic Yersinia enterocolitica in slaughtered pigs by cultural methods and real-time polymerase chain reaction"],"prefix":"10.4081","volume":"4","author":[{"given":"Rina","family":"Mazzette","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Fois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simonetta Gianna","family":"Consolati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Salza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Tedde","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Soro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Collu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Ladu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastiano","family":"Virgilio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Piras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2015,5,27]]},"container-title":["Italian Journal of Food Safety"],"original-title":[],"link":[{"URL":"http:\/\/www.pagepressjournals.org\/index.php\/ijfs\/article\/viewFile\/4579\/4631","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2015,6,15]],"date-time":"2015-06-15T08:26:18Z","timestamp":1434356778000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.pagepressjournals.org\/index.php\/ijfs\/article\/view\/ijfs.2015.4579"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,27]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,5,28]]}},"URL":"https:\/\/doi.org\/10.4081\/ijfs.2015.4579","relation":{},"ISSN":["2239-7132"],"issn-type":[{"value":"2239-7132","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,27]]}}}