{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:13:18Z","timestamp":1790615598637,"version":"4.1.0"},"reference-count":0,"publisher":"PAGEPress Publications","issue":"2","license":[{"start":{"date-parts":[[2016,5,2]],"date-time":"2016-05-02T00:00:00Z","timestamp":1462147200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ital J Food Safety"],"abstract":"<jats:p>This study was aimed at collecting data on presence, dissemination and persistence of &lt;em&gt;Pseudomonas&lt;\/em&gt; in small-scale dairy farms. Six farms (located in Piedmont) were visited three times over 2014: 116 waters (wells and different faucets\/pipes) and 117 environmental samples (milking equipments and drains) were collected. Enumeration of &lt;em&gt;Pseudomonadaceae&lt;\/em&gt; was performed, 3-5 colonies\/samples were selected for identification via 16SrDNA\/oprI polymerase chain reaction (PCR), and typed by enterobacterial-repetitive- intergenic-consensus (ERIC)-PCR. Pseudomonadaceae were detected in 77% of samples. No statistical differences were found among proportions of positives across farms, sample typologies and seasons. Most isolates were &lt;em&gt;Pseudomonas fluorescens&lt;\/em&gt; (45%), and ERIC-PCR showed 32 persistent types diffused across farms. All in all, &lt;em&gt;Pseudomonas&lt;\/em&gt; spp. represents a challenge, considering its presence over time in water as well as in teat cups, indicating a continuous source of contamination. Moreover, persistency of strains may indicate biofilm-formation and\/or sanitisers resistance, therefore emphasising the role of primary production for preventing milk contamination by &lt;em&gt;Pseudomonas&lt;\/em&gt; spp.<\/jats:p>","DOI":"10.4081\/ijfs.2016.5652","type":"journal-article","created":{"date-parts":[[2016,5,18]],"date-time":"2016-05-18T06:31:32Z","timestamp":1463553092000},"source":"Crossref","is-referenced-by-count":10,"title":["Dissemination and persistence of Pseudomonas spp. in small-scale dairy farms"],"prefix":"10.4081","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0978-8346","authenticated-orcid":false,"given":"Daniele Michele","family":"Nucera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Lomonaco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Morra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Francesco","family":"Ortoffi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Giaccone","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ausilia","family":"Grassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2549","published-online":{"date-parts":[[2016,5,2]]},"container-title":["Italian Journal of Food Safety"],"original-title":[],"link":[{"URL":"http:\/\/www.pagepressjournals.org\/index.php\/ijfs\/article\/viewFile\/5652\/5109","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T07:19:22Z","timestamp":1466752762000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.pagepressjournals.org\/index.php\/ijfs\/article\/view\/5652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,2]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2016,4,19]]}},"URL":"https:\/\/doi.org\/10.4081\/ijfs.2016.5652","relation":{},"ISSN":["2239-7132"],"issn-type":[{"value":"2239-7132","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,2]]}}}