{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T05:48:54Z","timestamp":1726465734256},"reference-count":0,"publisher":"Hematology Section, Dept. of Radiological Science and Hematology, Catholic University, Rome, Italy","issue":"1","license":[{"start":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T00:00:00Z","timestamp":1667001600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediterr J Hematol Infect Dis"],"abstract":"<jats:p>Background: The heterogeneity of coronavirus disease of 2019 (COVID-19) lies within its diverse symptoms and severity, ranging from mild to lethal. Acute respiratory distress syndrome (ARDS) has been shown to be a leading cause of mortality in COVID-19 patients, characterized by a hyper cytokine storm. Autoimmunity is proposed to occur as a result of COVID-19, given the high similarity of the immune responses observed in COVID-19 and autoimmune diseases. Here, we investigate the level of autoimmune antibodies in COVID-19 patients with different severities. Results: Initial screening for antinuclear antibodies (ANA) IgG using ELISA revealed that 1.58% (2\/126) and 4% (5\/126) of intensive care unit (ICU) COVID-19 cases expressed strong and moderate ANA levels, respectively. An additional sample was positive with immunofluorescence assays (IFA) screening. However, all the non-ICU cases (n=273) were ANA negative using both assays. Samples positive for ANA were further confirmed with large-scale autoantibody screening by phage immunoprecipitation-sequencing (PhIP-Seq). The majority of the ANA-positive samples showed \"speckled\" ANA pattern by microscopy, and revealed autoantibody specificities that predominantly targeted proteins involved in intracellular signal transduction, metabolism, apoptotic processes, and cell death by PhIP-Seq; further denoting reactivity to nuclear and cytoplasmic antigens. Conclusion: Our results further support the notion of routine screening for autoimmune responses in COVID-19 patients, which might help improve disease prognosis and patient management. Further, results provide compelling evidence that ANA-positive individuals should be excluded from being donors for convalescent plasma therapy in the context of COVID-19.<\/jats:p>","DOI":"10.4084\/mjhid.2022.076","type":"journal-article","created":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T10:59:35Z","timestamp":1667213975000},"page":"e2022076","source":"Crossref","is-referenced-by-count":4,"title":["DETECTION OF ANTINUCLEAR ANTIBODIES TARGETING INTRACELLULAR SIGNAL TRANSDUCTION, METABOLISM, APOPTOTIC PROCESSES AND CELL DEATH IN CRITICAL COVID-19 PATIENTS"],"prefix":"10.4084","volume":"14","author":[{"given":"HADI","family":"YASSINE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2554","published-online":{"date-parts":[[2022,10,29]]},"container-title":["Mediterranean Journal of Hematology and Infectious Diseases"],"original-title":[],"link":[{"URL":"https:\/\/mjhid.org\/index.php\/mjhid\/article\/download\/5112\/4249","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/mjhid.org\/index.php\/mjhid\/article\/download\/5112\/4262","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/mjhid.org\/index.php\/mjhid\/article\/download\/5112\/4267","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/mjhid.org\/index.php\/mjhid\/article\/download\/5112\/4268","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/mjhid.org\/index.php\/mjhid\/article\/download\/5112\/4249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,31]],"date-time":"2022-10-31T10:59:35Z","timestamp":1667213975000},"score":1,"resource":{"primary":{"URL":"https:\/\/mjhid.org\/index.php\/mjhid\/article\/view\/5112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,29]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2022,1,1]]}},"URL":"https:\/\/doi.org\/10.4084\/mjhid.2022.076","relation":{},"ISSN":["2035-3006"],"issn-type":[{"value":"2035-3006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,29]]}}}