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Autophagy, a self-digestion process, is upregulated during I\/R injury and strongly induced by hypoxia. This study aims to determine whether dexmedetomidine can decrease pancreatic \u03b2-cell damage by regulating autophagy under hypoxia.Methods: INS-1 rat insulinoma cells were cultured in dexmedetomidine before being exposed to cobalt chloride (CoCl2)-induced hypoxia. Cell viability and the expression of autophagy-related proteins (light chain 3B [LC3B]-II, p62, and ATGs) were assessed. The expression of apoptosis-related proteins (BCL-2 and P-BAD) were also evaluated. CoCl2-treated INS-1 cells were pretreated with the autophagosome formation inhibitor, 3-methyladenine (3-MA), to compare its effects with those of dexmedetomidine. Bafilomycin-A1 (Baf-A1) that inhibits autophagosome degradation was used to confirm the changes in autophagosome formation induced by dexmedetomidine.Results: Dexmedetomidine attenuated the increased expression of autophagic proteins (LC3B-II, p62, and ATGs) and reversed the CoCl2-induced reduction in the proliferation of INS-1 cells after hypoxia. Dexmedetomidine also alleviated the decreased expression of the anti-apoptotic protein (BCL-2) and the increased expression of apoptotic protein (BAX). Dexmedetomidine reduces the activation of autophagy through inhibiting autophagosome formation, as confirmed by a decrease in LC3B-II\/I ratio, a marker of autophagosome formation, in LC3B turnover assay combined with Baf-A1.Conclusions: Dexmedetomidine alleviates the degree of cellular damage in INS-1 cells against CoCl2-induced hypoxia by regulating autophagosome formation. These results provide a basis for further studies to confirm these effects in clinical practice.<\/jats:p>","DOI":"10.4097\/kja.24457","type":"journal-article","created":{"date-parts":[[2024,10,2]],"date-time":"2024-10-02T08:46:01Z","timestamp":1727858761000},"page":"623-634","update-policy":"https:\/\/doi.org\/10.4097\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Dexmedetomidine alleviates CoCl2-induced hypoxic cellular damage in INS-1 cells by regulating autophagy"],"prefix":"10.4097","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1398-3304","authenticated-orcid":true,"given":"Jin Ha","family":"Park","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5774-6346","authenticated-orcid":true,"given":"Ju 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