{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:02:14Z","timestamp":1785178934012,"version":"3.55.0"},"reference-count":20,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:00:00Z","timestamp":1617148800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr. J. Lab. Med."],"abstract":"<jats:p>Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis and Mycoplasma genitalium are the four main aetiologies of sexually transmitted infections responsible for vaginal discharge syndrome (VDS). Commercially available multiplex polymerase chain reaction (PCR) assays are expensive and generally not customisable. We evaluated a highly customisable singleplex PCR approach by testing it in parallel with the Anyplex\u2122 II STI-7 detection assay in a cohort of South African women that presented with VDS between May 2016 and January 2017. Our multiple singleplex PCR strategy proved to be a simple, accurate, rapid, affordable and scalable option for diagnosing VDS.<\/jats:p>","DOI":"10.4102\/ajlm.v10i1.1124","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T15:26:58Z","timestamp":1616858818000},"source":"Crossref","is-referenced-by-count":3,"title":["Performance of TaqMan probes for the detection of sexually transmitted infections in South African women"],"prefix":"10.4102","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0221-0808","authenticated-orcid":false,"given":"Nireshni","family":"Mitchev","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0407-4635","authenticated-orcid":false,"given":"Ravesh","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4530-234X","authenticated-orcid":false,"given":"Nigel","family":"Garrett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1590-9893","authenticated-orcid":false,"given":"Veron","family":"Ramsuran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2793-1855","authenticated-orcid":false,"given":"Abraham J.","family":"Niehaus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8436-3268","authenticated-orcid":false,"given":"Koleka P.","family":"Mlisana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2580","published-online":{"date-parts":[[2021,3,31]]},"reference":[{"key":"ref0001","author":"World Health Organization","year":"2019","journal-title":"Report on global sexually transmitted infection surveillance 2018 [homepage on the Internet]"},{"key":"ref0002","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2015.01.011"},{"key":"ref0003","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.18.228486"},{"key":"ref0004","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002511"},{"key":"ref0005","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3380-6"},{"key":"ref0006","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr6404a1"},{"key":"ref0007","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0196209"},{"key":"ref0008","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-052581"},{"key":"ref0009","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-011-0362-7"},{"key":"ref0010","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2013.07.011"},{"key":"ref0011","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0000000000000239"},{"key":"ref0012","first-page":"1","volume":"63","author":"Papp","year":"2014","journal-title":"MMWR Recomm Rep"},{"key":"ref0013","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-015-1029-2"},{"key":"ref0014","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2018-026888"},{"key":"ref0015","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2016-052581"},{"key":"ref0016","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.f5605"},{"key":"ref0017","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19991130)18:22<2987::AID-SIM205>3.0.CO;2-B"},{"key":"ref0018","doi-asserted-by":"publisher","DOI":"10.1186\/1742-6405-11-31"},{"key":"ref0019","author":"Centers for Disease Control and Prevention","year":"2016","journal-title":"Sexually transmitted disease surveillance report [homepage on the Internet]"},{"key":"ref0020","doi-asserted-by":"publisher","DOI":"10.1586\/erm.12.60"}],"container-title":["African Journal of Laboratory Medicine"],"original-title":[],"link":[{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1124\/1934","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1124\/1933","content-type":"application\/epub+zip","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1124\/1935","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1124\/1932","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1124\/1932","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,1]],"date-time":"2021-04-01T00:13:21Z","timestamp":1617236001000},"score":1,"resource":{"primary":{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/view\/1124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,31]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,2,11]]}},"URL":"https:\/\/doi.org\/10.4102\/ajlm.v10i1.1124","relation":{},"ISSN":["2225-2010","2225-2002"],"issn-type":[{"value":"2225-2010","type":"electronic"},{"value":"2225-2002","type":"print"}],"subject":[],"published":{"date-parts":[[2021,3,31]]}}}