{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T12:12:35Z","timestamp":1747224755998,"version":"3.40.5"},"reference-count":21,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T00:00:00Z","timestamp":1617062400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr. J. Lab. Med."],"abstract":"<jats:p>Background: Molecular detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is at the forefront of the global response to the coronavirus disease 2019 (COVID-19) pandemic. However, molecular diagnostic capabilities are poorly developed in many African countries. Efforts by the Nigeria Centre for Disease Control and other public health agencies to scale up facilities for molecular testing across the continent are well documented, but there are few accounts from the laboratories at the frontline.Intervention: As part of an institutional response to the COVID-19 pandemic, the University of Benin Teaching Hospital, Benin City, Nigeria, signed a memorandum of understanding with a World Bank-supported institution to obtain a non-proprietary testing platform, renovated an existing molecular virology laboratory and validated the test process to make SARS-CoV-2 testing readily available for decision-making by frontline health workers. These efforts resulted in the University of Benin Teaching Hospital\u2019s inclusion in the Nigeria Centre for Disease Control COVID-19 molecular laboratory network. The laboratory achieved a turnover of 12 123 tests within 7 months of operation. Challenges faced and dealt with include incompatible equipment, limited skilled manpower, unstable (unreliable) electric power supply, disrupted procurement and supply chain, and significant overhead costs.Lessons learnt: Molecular diagnostic capability is essential in laboratory preparedness for pandemic response and can be achieved by establishing collaborative networks in low-resource settings.Recommendations: Molecular diagnostic capabilities attained during the COVID-19 pandemic should be maintained by governmental support of the local biotechnology sector, collaboration with partners and stakeholders and the expansion of diagnostics to include other diseases of public health importance.<\/jats:p>","DOI":"10.4102\/ajlm.v10i1.1326","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T15:27:20Z","timestamp":1616858840000},"source":"Crossref","is-referenced-by-count":3,"title":["Setting up a molecular diagnostic laboratory for SARS-CoV-2 testing: Experience of a single centre in a resource-constrained setting"],"prefix":"10.4102","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8111-0743","authenticated-orcid":false,"given":"Iriagbonse I.","family":"Osaigbovo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2719-0188","authenticated-orcid":false,"given":"Isaac O.","family":"Igbarumah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3272-038X","authenticated-orcid":false,"given":"Ekene B.","family":"Muoebonam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8222-1592","authenticated-orcid":false,"given":"Darlington E.","family":"Obaseki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2580","published-online":{"date-parts":[[2021,3,30]]},"reference":[{"key":"ref0001","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001017"},{"journal-title":"Coronavirus disease (COVID-19) weekly update on COVID-19, 9th October","year":"2020","author":"World Health Organization","key":"ref0002"},{"key":"ref0003","doi-asserted-by":"publisher","DOI":"10.1080\/23288604.2020.1753464"},{"key":"ref0004","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1745095"},{"key":"ref0005","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPELMG6GX6RQSM"},{"key":"ref0006","doi-asserted-by":"publisher","DOI":"10.3201\/eid1110.041343"},{"key":"ref0007","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001839"},{"key":"ref0008","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2018-000712"},{"key":"ref0009","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.02.049"},{"journal-title":"National strategy to scale up access to coronavirus disease testing in Nigeria [homepage on the Internet]","year":"2020","author":"Nigeria Centre for Disease Control","key":"ref0010"},{"key":"ref0011","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-020-01622-w"},{"key":"ref0012","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa071"},{"journal-title":"About UBTH [homepage on the Internet]","author":"University of Benin Teaching Hospital","key":"ref0013"},{"journal-title":"Progression of COVID-19 cases in Nigeria","author":"Nigeria Centre for Disease Control","key":"ref0014"},{"key":"ref0015","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqaa052"},{"key":"ref0016","doi-asserted-by":"publisher","DOI":"10.3201\/eid2608.201843"},{"key":"ref0017","doi-asserted-by":"publisher","DOI":"10.12688\/aasopenres.13061.1"},{"journal-title":"bioRxiv preprint","year":"2020","author":"Becker","key":"ref0018"},{"key":"ref0019","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqw083"},{"journal-title":"World Health Organization model list of essential in vitro diagnostics [homepage on the Internet]","author":"World Health Organization 2018","key":"ref0020"},{"key":"ref0021","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0000000000000240"}],"container-title":["African Journal of Laboratory Medicine"],"original-title":[],"link":[{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1326\/1930","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1326\/1929","content-type":"application\/epub+zip","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1326\/1931","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1326\/1928","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/viewFile\/1326\/1928","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,31]],"date-time":"2021-03-31T00:13:10Z","timestamp":1617149590000},"score":1,"resource":{"primary":{"URL":"https:\/\/ajlmonline.org\/index.php\/ajlm\/article\/view\/1326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,30]]},"references-count":21,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,2,11]]}},"URL":"https:\/\/doi.org\/10.4102\/ajlm.v10i1.1326","relation":{},"ISSN":["2225-2010","2225-2002"],"issn-type":[{"type":"electronic","value":"2225-2010"},{"type":"print","value":"2225-2002"}],"subject":[],"published":{"date-parts":[[2021,3,30]]}}}