{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T01:50:08Z","timestamp":1778291408304,"version":"3.51.4"},"reference-count":22,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2023,4,12]],"date-time":"2023-04-12T00:00:00Z","timestamp":1681257600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr. J. Lab. Med."],"abstract":"<jats:p>Healthcare-associated infections (HCAIs) caused by extended spectrum \u03b2-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (blaCTX-M,\u00a0blaTEM\u00a0and\u00a0blaSHV) among 30 ceftriaxone-resistant GNB causing HCAIs between January 2022 and July 2022 by multiplex polymerase chain reaction assay at the zonal referral hospital in Mwanza, Tanzania. Twenty-five (83.3%) had at least one ESBL gene, of which 23\/25 (92.0%) carried the\u00a0blaCTX-M\u00a0gene. Seventy-two percent (18\/25) of the GNB-ESBL isolates carried more than one ESBL gene, of which the majority (88.8%;\u00a0n\u00a0= 16\/25) carried the\u00a0blaCTX-M\u00a0and\u00a0blaTEM\u00a0genes. Extended spectrum \u03b2-lactamase genes, particularly\u00a0blaCTX-M, are common among ceftriaxone-resistant GNB causing HCAIs.What this study adds:\u00a0This study revealed the distribution of genes (blaCTX-M,\u00a0blaTEM\u00a0and\u00a0blaSHV) coding for ESBL production among ceftriaxone resistant GNB causing HCAIs However, all ESBL producing GNB were susceptible towards ceftriaxone-sulbactam indicating that ceftriaxone-sulbactam may be empirically prescribed for treating patients with HCAIs.<\/jats:p>","DOI":"10.4102\/ajlm.v12i1.2107","type":"journal-article","created":{"date-parts":[[2023,4,8]],"date-time":"2023-04-08T11:51:22Z","timestamp":1680954682000},"source":"Crossref","is-referenced-by-count":4,"title":["Characterisation of genes encoding for extended spectrum \u03b2-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania"],"prefix":"10.4102","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3733-0404","authenticated-orcid":false,"given":"Jenipher G.","family":"Mwakyabala","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7962-3443","authenticated-orcid":false,"given":"Conjester I.","family":"Mtemisika","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5088-3150","authenticated-orcid":false,"given":"Stacy","family":"Mshana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7560-3012","authenticated-orcid":false,"given":"Adam A.","family":"Mwakyoma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4178-3254","authenticated-orcid":false,"given":"Vitus","family":"Silago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2580","published-online":{"date-parts":[[2023,4,12]]},"reference":[{"issue":"1","key":"ref0","doi-asserted-by":"publisher","first-page":"67","DOI":"10.5455\/2394-6040.ijcmph20141114","article-title":"Prevention of healthcare-associated infections: Protecting patients, saving lives","volume":"1","author":"Danasekaran","year":"2014","unstructured":"Danasekaran R, Mani G, Annadurai K. 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