{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:06:19Z","timestamp":1790823979263,"version":"4.1.0"},"reference-count":139,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T00:00:00Z","timestamp":1748649600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The World Health Organization identified mpox (formerly known as monkeypox), as a resurgent zoonotic epidemic caused by the mpox virus. It is an emerging and re-emerging pathogen with a range of hosts and geographical distribution worldwide. Peer-reviewed scientific articles from 1958 to 29 August 2024 related to global mpox research were extracted from Web of ScienceTM Core Collection and Google Scholar Databases to gauge the extent of the infection. Mpox is marked by a recent resurgence of infections across continents, with Africa being the hardest-hit region. The mpox re-emergence has shown a new mechanism of transmission, with several causes such as a rise in the number of unvaccinated individuals, behaviour risk factors, waning immunity, genetic evolution, and environmental circumstances. Preventive and control measures of mpox include vaccination and patient isolation, while treatment involves antivirals and antibiotics for secondary bacterial infections. Laboratory diagnosis entailing polymerase chain reaction can be effective for routine purposes, but results of serological tests must be interpreted with caution, because of cross-reacting determinants among orthopoxviruses. The structure and classification of the mpox virus, clinical manifestations, pathophysiology, epidemiology, historical antecedent, therapeutics, vaccines, and laboratory diagnosis of the disease are explicated, showcasing mpox as an emerging or re-emerging infection with a potential colossal burden on healthcare, and its classification as an international public health emergency by the World Health Organization.\n\nWhat this study adds: This review provides the global situation of mpox as an emerging or re-emerging infection, warranting its designation as an international public health emergency.<\/jats:p>","DOI":"10.4102\/ajlm.v14i1.2644","type":"journal-article","created":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T09:28:41Z","timestamp":1748683721000},"update-policy":"https:\/\/doi.org\/10.4102\/crossmark-policy","source":"Crossref","is-referenced-by-count":3,"title":["Mpox: An emerging or re-emerging infection with a potential colossal burden on healthcare globally"],"prefix":"10.4102","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0068-2035","authenticated-orcid":false,"given":"Chikwelu L.","family":"Obi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003hsr719","id-type":"ROR","asserted-by":"publisher"}],"name":"Sefako Makgatho Health Sciences University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3631-4522","authenticated-orcid":false,"given":"Nqobile M.","family":"Mkolo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003hsr719","id-type":"ROR","asserted-by":"publisher"}],"name":"Sefako Makgatho Health Sciences University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6112-5478","authenticated-orcid":false,"given":"Liziwe L.","family":"Mugivhisa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003hsr719","id-type":"ROR","asserted-by":"publisher"}],"name":"Sefako Makgatho Health Sciences University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6599-0813","authenticated-orcid":false,"given":"Modupe O.","family":"Ogunrombi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003hsr719","id-type":"ROR","asserted-by":"publisher"}],"name":"Sefako Makgatho Health Sciences University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0535-9648","authenticated-orcid":false,"given":"Mukhethwa M.","family":"Mphephu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003hsr719","id-type":"ROR","asserted-by":"publisher"}],"name":"Sefako Makgatho Health Sciences University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9164-8213","authenticated-orcid":false,"given":"Clarissa M.","family":"Naidoo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/003hsr719","id-type":"ROR","asserted-by":"publisher"}],"name":"Sefako Makgatho Health Sciences University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2580","published-online":{"date-parts":[[2025,5,31]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"Ogunleye SC, Akinsulie OC, Aborode AT, et al. The re-emergence and transmission of Monkeypox virus in Nigeria: The role of one health. Front Public Health. 2024;11:1334238. https:\/\/doi.org\/10.3389\/fpubh.2023.1334238","DOI":"10.3389\/fpubh.2023.1334238"},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Zinnah MA, Uddin MB, Hasan T, et al. The re-emergence of Mpox: Old illness, modern challenges. Biomedicines. 2024;12(7):1457. https:\/\/doi.org\/10.3390\/biomedicines12071457","DOI":"10.3390\/biomedicines12071457"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Araf Y, Nipa JF, Naher S, et al. Insights into the transmission, host range, genomics, vaccination, and current epidemiology of the monkeypox virus. Vet Med Int. 2024;2024(1):8839830. https:\/\/doi.org\/10.1155\/2024\/8839830","DOI":"10.1155\/2024\/8839830"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Lim CK, Roberts J, Moso M, et al. Mpox diagnostics: Review of current and emerging technologies. J Med Virol. 2023;95(1):e28429. doi: 10.1002\/jmv.28429. Erratum in: J Med Virol. 2023;95(2):e28581. https:\/\/doi.org\/10.1002\/jmv.28429","DOI":"10.1002\/jmv.28429"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Zandi M, Shafaati M, Hosseini F. Mechanisms of immune evasion of monkeypox virus. Front Microbiol. 2023;14:1106247. https:\/\/doi.org\/10.3389\/fmicb.2023.1106247","DOI":"10.3389\/fmicb.2023.1106247"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Alizadeh I, Shafaati M, Zandi M. Is it possible that arthropods could be an accidental reservoir for the outbreak of mpox virus in 2022? New Microbes New Infect. 2023;53:101138. https:\/\/doi.org\/10.1016\/j.nmni.2023.101138","DOI":"10.1016\/j.nmni.2023.101138"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Zebardast A, Latifi T, Shafiei-Jandaghi NZ, Gholami Barzoki M, Shatizadeh Malekshahi S. Plausible reasons for the resurgence of Mpox (formerly Monkeypox): An overview. Trop Dis Travel Med Vaccines. 2023;9(1):23. https:\/\/doi.org\/10.1186\/s40794-023-00209-6","DOI":"10.1186\/s40794-023-00209-6"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Gupta AK, Talukder M, Rosen T, Piguet V. Differential diagnosis prevention and treatment of mpox (monkeypox) a review for dermatologists. Am J Clin Dermatol. 2023;24(4)541\u2013556. https:\/\/doi.org\/10.1007\/s40257-023-00778-4","DOI":"10.1007\/s40257-023-00778-4"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Kuehn R, Fox T, Guyatt G, Lutje V, Gould S. Infection prevention and control measures to reduce the transmission of mpox a systematic review. PLoS Global Public Health. 2024;4(1):e0002731. https:\/\/doi.org\/10.1371\/journal.pgph.0002731","DOI":"10.1371\/journal.pgph.0002731"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Alissa M, Alghamdi A, Alghamdi SA. Overview of reemerging mpox infection with a focus on neurological manifestations. Rev Med Virol. 2024;34(2):e2527. https:\/\/doi.org\/10.1002\/rmv.2527","DOI":"10.1002\/rmv.2527"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Bruno G, Buccoliero GB. Antivirals against monkeypox (Mpox) in humans: An updated narrative review. Life. 2023;13(10):1969. https:\/\/doi.org\/10.3390\/life13101969","DOI":"10.3390\/life13101969"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Sham S, Sapna FN, Anjali FN, et al. The changing global epidemiology of re-emerging human monkeypox virus infection: A systematic review. Cureus. 2023;15(9):e45123. https:\/\/doi.org\/10.7759\/cureus.45123","DOI":"10.7759\/cureus.45123"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Qudus MS, Cui X, Tian M, et al. The prospective outcome of the monkeypox outbreak in 2022 and characterization of monkeypox disease immunobiology. Front Cell Infect Microbiol. 2023;13:1196699. https:\/\/doi.org\/10.3389\/fcimb.2023.1284014","DOI":"10.3389\/fcimb.2023.1284014"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Stumbar SE, Nusynowitz J, Jamneshan L, Samuels M, Garba NA. The need for emerging diseases curricula: A case study of Mpox. PRiMER. 2022;6:922238. https:\/\/doi.org\/10.22454\/PRiMER.2022.922238","DOI":"10.22454\/PRiMER.2022.922238"},{"key":"ref14","unstructured":"International Committee on Taxonomy of Viruses [homepage on the Internet]. 2022 [cited 2024 Sept 08]. Available from: https\/\/ictv.global\/taxonomy"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Likos AM, Sammons SA, Olson VA, et al. A tale of two clades: Monkeypox viruses. J Gen Virol. 2005;86(Pt 10):2661\u20132672. https:\/\/doi.org\/10.1099\/vir.0.81215-0","DOI":"10.1099\/vir.0.81215-0"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Alakunle E, Moens U, Nchinda G, Okeke MI. Monkeypox virus in Nigeria infection biology epidemiology and evolution. Viruses. 2020;12(11):1257. https:\/\/doi.org\/10.3390\/v12111257","DOI":"10.3390\/v12111257"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Monz\u00f3n S, Varona S, Negredo A, et al. Monkeypox virus genomic accordion strategies. Nat Commun. 2024;15:3059. https:\/\/doi.org\/10.1038\/s41467-024-46949-7","DOI":"10.1038\/s41467-024-46949-7"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Karagoz A, Tombuloglu H, Alsaeed M, et al. Monkeypox (mpox) virus: Classification, origin, transmission, genome organization, antiviral drugs, and molecular diagnosis. J Infect Public Health. 2023;16(4):531\u2013541. https:\/\/doi.org\/10.1016\/j.jiph.2023.02.003","DOI":"10.1016\/j.jiph.2023.02.003"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Soheili M, Nasseri S, Afraie M, et al. Monkeypox: Virology, pathophysiology, clinical characteristics, epidemiology, vaccines, diagnosis, and treatments. J Pharm Pharm Sci. 2022;25:297\u2013322. https:\/\/doi.org\/10.18433\/jpps33138","DOI":"10.18433\/jpps33138"},{"key":"ref20","unstructured":"World Health Organization. 2022\u201324 Mpox (Monkeypox) outbreak: Global trends World Health Organization [homepage on the Internet]. 2024 [cited 2024 Sept 18]. Available from: https:\/\/worldhealthorg.shinyapps.io\/mpx_global\/"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Happi C, Adetifa I, Mbala P, et al. Urgent need for a non-discriminatory and non-stigmatizing nomenclature for monkeypox virus. PLoS Biol. 2022;20(8):e3001769. https:\/\/doi.org\/10.1371\/journal.pbio.3001769","DOI":"10.1371\/journal.pbio.3001769"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Lum FM, Torres-Ruesta A, Tay MZ, et al. Monkeypox: Disease epidemiology, host immunity and clinical interventions. Nat Rev Immunol. 2022;22:597\u2013613. https:\/\/doi.org\/10.1038\/s41577-022-00775-4","DOI":"10.1038\/s41577-022-00775-4"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Gal\u00e1n-Huerta KA, Paz-Infanzon M, Nuzzolo-Shihadeh L, et al. Metagenomic sequencing of monkeypox virus, Northern Mexico. Emerg Infect Dis. 2023;29(2):448\u2013450. https:\/\/doi.org\/10.3201\/eid2902.221199","DOI":"10.3201\/eid2902.221199"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Berthet N, Descorps-Decl\u00e8re S, Besombes C, et al. Genomic history of human monkey pox infections in the Central African Republic between 2001 and 2018. Sci Rep. 2021;11(1):13085. https:\/\/doi.org\/10.1038\/s41598-021-92315-8","DOI":"10.1038\/s41598-021-92315-8"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Isidro J, Borges V, Pinto M, et al. Phylogenomic characterization and signs of microevolution in the 2022 multi-country outbreak of monkeypox virus. Nat Med. 2022;28(8):1569\u20131572. https:\/\/doi.org\/10.1038\/s41591-022-01907-y","DOI":"10.1038\/s41591-022-01907-y"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"O\u2019Toole \u00c1, Neher RA, Ndodo N, et al. APOBEC3 deaminase editing in mpox virus as evidence for sustained human transmission since at least 2016. Science. 2023;382(6670):595\u2013600. https:\/\/doi.org\/10.1126\/science.adg8116","DOI":"10.1126\/science.adg8116"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Kumar N, Acharya A, Gendelman HE, Byrareddy SN. The 2022 outbreak and the pathobiology of the monkeypox virus. J Autoimmunity. 2022;131:102855. https:\/\/doi.org\/10.1016\/j.jaut.2022.102855","DOI":"10.1016\/j.jaut.2022.102855"},{"key":"ref28","unstructured":"Paharia T, Paharia PT. Insights into the biology of the monkeypox virus [homepage on the Internet]. News-Medical; 2022 [cited 2024 Sept 10]. Available from: https\/\/www.news-medical.net\/news\/20220823\/Insights-into-the-biology-of-the-monkeypox-virus.aspx"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Elde NC, Child SJ, Eickbush MT, et al. Poxviruses deploy genomic accordions to adapt rapidly against host antiviral defenses. Cell. 2012;150(4):831\u2013841. https:\/\/doi.org\/10.1016\/j.cell.2012.05.049","DOI":"10.1016\/j.cell.2012.05.049"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Hendrickson RC, Wang C, Hatcher EL, Lefkowitz EJ. Orthopoxvirus genome evolution the role of gene loss. Viruses. 2010;2(9):1933\u20131967. https:\/\/doi.org\/10.3390\/v2091933","DOI":"10.3390\/v2091933"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Kaler J, Hussain A, Flores G, Kheiri S, Desrosiers D. Monkeypox a comprehensive review of transmission pathogenesis and manifestation. Cureus. 2022;14(7):e26531. https:\/\/doi.org\/10.7759\/cureus.26531","DOI":"10.7759\/cureus.26531"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Sklenovsk\u00e1 N, Van Ranst M. Emergence of monkeypox as the most important orthopoxvirus infection in humans. Front Public Health. 2018;6:241. https:\/\/doi.org\/10.3389\/fpubh.2018.00241","DOI":"10.3389\/fpubh.2018.00241"},{"key":"ref33","unstructured":"U.S. Monkeypox Investigation CDC 2022 [homepage on the Internet]. [cited 2024 Sept 08]. Available from: https:\/\/www.cdc.gov\/poxvirus\/mpox\/response\/2022\/us-map.html"},{"key":"ref34","unstructured":"Moore M. Monkeypox. ISITIFS vol. 22. Bethesda, Maryland: National Library of Medicine; 2022."},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"Parker S, Buller RM. A review of experimental and natural infections of animals with monkeypox virus between 1958 and 2012. Fut Virol. 2013;8(2):129\u2013157. https:\/\/doi.org\/10.2217\/fvl.12.130","DOI":"10.2217\/fvl.12.130"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"Van Dijck C, Hoff NA, Mbala-Kingebeni P, et al. Emergence of mpox in the post-smallpox era-a narrative review on mpox epidemiology. Clin Microbiol Infect. 2023;29(12):1487\u20131492. https:\/\/doi.org\/10.1016\/j.cmi.2023.08.008","DOI":"10.1016\/j.cmi.2023.08.008"},{"key":"ref37","unstructured":"McMullen CL. A one health perspective on disease dynamics: Human monkeypox transmission in Sankuru district, Democratic Republic of Congo. Master\u2019s thesis, Duke University; 2015. https:\/\/hdl.handle.net\/10161\/10046"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"Bunge EM, Hoet B, Chen L, et al. The changing epidemiology of human monkeypox-A potential threat? A systematic review. PLoS Negl Trop Dis. 2022;16(2):e0010141. https:\/\/doi.org\/10.1371\/journal.pntd.0010141","DOI":"10.1371\/journal.pntd.0010141"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"Deng X, Tian Y, Zou J, Yang J, Sun K, Yu H. The risk of mpox importation and subsequent outbreak potential in Chinese mainland: A retrospective statistical modelling study. Infect Dis Poverty. 2024;13(01):63\u201373. https:\/\/doi.org\/10.1186\/s40249-024-01189-1","DOI":"10.1186\/s40249-024-01189-1"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"Thornhill JP, Barkati S, Walmsley S, et al. Monkeypox virus infection in humans across 16 countries: April\u2013June 2022. N Engl J Med. 2022;387(8):679\u2013691. https:\/\/doi.org\/10.1056\/NEJMoa2207323","DOI":"10.1056\/NEJMc2213969"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"Shchelkunova GA, Shchelkunov SN. Smallpox, monkeypox, and other human orthopoxvirus infections. Viruses. 2022;15(1):103. https:\/\/doi.org\/10.3390\/v15010103","DOI":"10.3390\/v15010103"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"Beer EM, Rao VB. A systematic review of the epidemiology of human monkeypox outbreaks and implications for outbreak strategy. PLoS Negl Trop Dis. 2019;13(10):e0007791. https:\/\/doi.org\/10.1371\/journal.pntd.0007791","DOI":"10.1371\/journal.pntd.0007791"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"Guan H, Gul I, Xiao C, et al. Emergence, phylogeography, and adaptive evolution of mpox virus. New Microbes New Infect. 2023;52:101102. https:\/\/doi.org\/10.1016\/j.nmni.2023.101102","DOI":"10.1016\/j.nmni.2023.101102"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"Koplow DA. Smallpox: The fight to eradicate a global scourge. Berkeley (CA): University of California Press; 2003.","DOI":"10.1525\/9780520938083"},{"key":"ref45","doi-asserted-by":"crossref","unstructured":"Artenstein AW, Grabenstein JD. Smallpox vaccines for biodefense: Need and feasibility. Expert Rev Vaccines. 2008;7(8):1225\u20131237. https:\/\/doi.org\/10.1586\/14760584.7.8.1225","DOI":"10.1586\/14760584.7.8.1225"},{"key":"ref46","doi-asserted-by":"crossref","unstructured":"Heymann DL, Szczeniowski M, Esteves K. Re-emergence of monkeypox in Africa: A review of the past six years. Br Med Bull. 1998;54(3):693\u2013702. https:\/\/doi.org\/10.1093\/oxfordjournals.bmb.a011720","DOI":"10.1093\/oxfordjournals.bmb.a011720"},{"key":"ref47","doi-asserted-by":"crossref","unstructured":"Hussain A, Kaler J, Lau G, Maxwell T. Clinical conundrums: Differentiating monkeypox from similarly presenting infections. Cureus. 2022;14(10):e29929. https:\/\/doi.org\/10.7759\/cureus.29929","DOI":"10.7759\/cureus.29929"},{"key":"ref48","unstructured":"Nwokike J. Regulatory reliance and post-marketing surveillance systems for safe and accelerated introduction of new medical products in low- and middle-income countries. Dissertation. Plymouth: University of Plymouth; 2023."},{"key":"ref49","doi-asserted-by":"crossref","unstructured":"Elsayed S, Bondy L, Hanage WP. Monkeypox virus infections in humans. Clin Microbiol Rev. 2022;35(4):e00092-22. https:\/\/doi.org\/10.1128\/cmr.00092-22","DOI":"10.1128\/cmr.00092-22"},{"key":"ref50","doi-asserted-by":"crossref","unstructured":"Siegrist EA, Sassine J. Antivirals with activity against mpox: A clinically oriented review. Clin Infect Dis. 2023;76(1):155\u2013164. https:\/\/doi.org\/10.1093\/cid\/ciac622","DOI":"10.1093\/cid\/ciac622"},{"key":"ref51","doi-asserted-by":"crossref","unstructured":"Islam MA, Mumin J, Haque MM, et al. Monkeypox virus (MPXV): A brief account of global spread, epidemiology, virology, clinical features, pathogenesis, and therapeutic interventions. Infect Med. 2023;2(4):262\u2013272. https:\/\/doi.org\/10.1016\/j.imj.2023.11.001","DOI":"10.1016\/j.imj.2023.11.001"},{"key":"ref52","doi-asserted-by":"crossref","unstructured":"Suner C, Ubals M, Tar\u00edn-Vicente EJ, et al. Viral dynamics in patients with monkeypox infection: A prospective cohort study in Spain. Lancet Infect Dis. 2023;23(4):445\u2013453.","DOI":"10.1016\/S1473-3099(22)00794-0"},{"key":"ref53","doi-asserted-by":"crossref","unstructured":"Guarner J, Del Rio C, Malani PN. Monkeypox in 2022\u2014What clinicians need to know. JAMA. 2022;328(2):139\u2013140. https:\/\/doi.org\/10.1001\/jama.2022.10802","DOI":"10.1001\/jama.2022.10802"},{"key":"ref54","doi-asserted-by":"crossref","unstructured":"Duarte PM, Adesola RO, Priyadarsini S, et al. Unveiling the global Surge of Mpox (Monkeypox): A comprehensive review of current evidence. Microbe. 2024;4:100141. https:\/\/doi.org\/10.1016\/j.microb.2024.100141","DOI":"10.1016\/j.microb.2024.100141"},{"key":"ref55","doi-asserted-by":"crossref","unstructured":"Zong Y, Kamoi K, Zhang J, Yang M, Ohno-Matsui K. Mpox (monkeypox) and the eye: Ocular manifestation, diagnosis, treatment, and vaccination. Viruses. 2023;15(3):616. https:\/\/doi.org\/10.3390\/v15030616","DOI":"10.3390\/v15030616"},{"key":"ref56","doi-asserted-by":"crossref","unstructured":"Caldeira MB, Neves JM, Pestana M, et al. Demographic and clinical characteristics of mpox patients attending an STD clinic in Lisbon. Int J Environ Res Public Health. 2023;20(19):6803. https:\/\/doi.org\/10.3390\/ijerph20196803","DOI":"10.3390\/ijerph20196803"},{"key":"ref57","doi-asserted-by":"crossref","unstructured":"Zeyen C, Kinberger M, Kriedemann H, et al. Clinical spectrum and long-term outcomes of mpox: A cohort study spanning from acute infection to six-month follow-up. BMC Infect Dis. 2024;24(1):317. https:\/\/doi.org\/10.1186\/s12879-024-09191-6","DOI":"10.1186\/s12879-024-09191-6"},{"key":"ref58","doi-asserted-by":"crossref","unstructured":"Selvaraj N, Shyam S, Dhurairaj P, et al. Mpox epidemiology, clinical manifestations, and recent developments in treatment and prevention. Expert Rev Anti Infect Ther. 2023;21(6):641\u2013653. https:\/\/doi.org\/10.1080\/14787210.2023.2208346","DOI":"10.1080\/14787210.2023.2208346"},{"key":"ref59","doi-asserted-by":"crossref","unstructured":"Musuka G, Moyo E, Tungwarara N, et al. A critical review of mpox outbreaks, risk factors, and prevention efforts in Africa: Lessons learned and evolving practices. IJID Reg. 2024;6:100402. https:\/\/doi.org\/10.1016\/j.ijregi.2024.100402","DOI":"10.1016\/j.ijregi.2024.100402"},{"key":"ref60","doi-asserted-by":"crossref","unstructured":"Shamim MA, Padhi BK, Satapathy P, et al. The use of antivirals in the treatment of human monkeypox outbreaks: A systematic review. Int J Infect Dis. 2023;127:150\u2013161. https:\/\/doi.org\/10.1016\/j.ijid.2022.11.040","DOI":"10.1016\/j.ijid.2022.11.040"},{"key":"ref61","doi-asserted-by":"crossref","unstructured":"Bhardwaj P, Sarkar S, Mishra R. Mpox and related poxviruses: A literature review of evolution, pathophysiology, and clinical manifestations. Asian Pac J Trop Biomed. 2024;14(8):319\u2013330. https:\/\/doi.org\/10.4103\/apjtb.apjtb_193_24","DOI":"10.4103\/apjtb.apjtb_193_24"},{"key":"ref62","doi-asserted-by":"crossref","unstructured":"De Clercq E. Cidofovir in the treatment of poxvirus infections. Antiviral Res. 2002;55(1):1\u20133. https:\/\/doi.org\/10.1016\/S0166-3542(02)00008-6","DOI":"10.1016\/S0166-3542(02)00008-6"},{"key":"ref63","doi-asserted-by":"crossref","unstructured":"Hostetler KY. Alkoxyalkyl prodrugs of acyclic nucleoside phosphonates enhance oral antiviral activity and reduce toxicity: Current state of the art. Antiviral Res. 2009;82(2):A84\u2013A98. https:\/\/doi.org\/10.1016\/j.antiviral.2009.01.005","DOI":"10.1016\/j.antiviral.2009.01.005"},{"key":"ref64","doi-asserted-by":"crossref","unstructured":"Yu J, Raj SM. Efficacy of three key antiviral drugs used to treat orthopoxvirus infections: A systematic review. Glob Biosecur. 2019;1(1):28\u201374. https:\/\/doi.org\/10.31646\/gbio.12","DOI":"10.31646\/gbio.12"},{"key":"ref65","doi-asserted-by":"crossref","unstructured":"Quenelle DC, Collins DJ, Kern ER. Efficacy of multiple- or single-dose cidofovir against vaccinia and cowpox virus infections in mice. Antimicrob Agents Chemother. 2003;47(10):3275\u20133280. https:\/\/doi.org\/10.1128\/AAC.47.10.3275-3280.2003","DOI":"10.1128\/AAC.47.10.3275-3280.2003"},{"key":"ref66","doi-asserted-by":"crossref","unstructured":"Wei H, Huang D, Fortman J, Wang R, Shao L, Chen ZW. Coadministration of cidofovir and smallpox vaccine reduced vaccination side effects but interfered with vaccine-elicited immune responses and immunity to monkeypox. J Virol. 2009;83(2):1115\u20131125. https:\/\/doi.org\/10.1128\/JVI.00984-08","DOI":"10.1128\/JVI.00984-08"},{"key":"ref67","doi-asserted-by":"crossref","unstructured":"Kern ER, Hartline C, Harden E, et al. Enhanced inhibition of orthopoxvirus replication in vitro by alkoxyalkyl esters of cidofovir and cyclic cidofovir. Antimicrob Agents Chemother. 2002;46(4):991\u2013995. https:\/\/doi.org\/10.1128\/AAC.46.4.991-995.2002","DOI":"10.1128\/AAC.46.4.991-995.2002"},{"key":"ref68","doi-asserted-by":"crossref","unstructured":"Quintana-Castanedo L, Tar\u00edn-Vicente EJ, Chiloeches-Fern\u00e1ndez C, Sendagorta-Cud\u00f3s E, Herranz-Pinto P. Recalcitrant molluscum contagiosum successfully treated with intralesional cidofovir in a patient with HIV\/AIDS. Int J Dermatol. 2021;60(3):368\u2013370. https:\/\/doi.org\/10.1111\/ijd.15210","DOI":"10.1111\/ijd.15210"},{"key":"ref69","doi-asserted-by":"crossref","unstructured":"Tippin TK, Morrison ME, Brundage TM, Momm\u00e9ja-Marin H. Brincidofovir is not a substrate for the human organic anion transporter 1: A mechanistic explanation for the lack of nephrotoxicity observed in clinical studies. Ther Drug Monit. 2016;38(6):777\u2013786. https:\/\/doi.org\/10.1097\/FTD.0000000000000353","DOI":"10.1097\/FTD.0000000000000353"},{"key":"ref70","doi-asserted-by":"crossref","unstructured":"Parker S, Touchette E, Oberle C, et al. Efficacy of therapeutic intervention with an oral ether\u2013lipid analogue of cidofovir (CMX001) in a lethal mousepox model. Antiviral Res. 2008;77(1):39\u201349. https:\/\/doi.org\/10.1016\/j.antiviral.2007.08.003","DOI":"10.1016\/j.antiviral.2007.08.003"},{"key":"ref71","doi-asserted-by":"crossref","unstructured":"Trost LC, Rose ML, Khouri J, et al. The efficacy and pharmacokinetics of brincidofovir for the treatment of lethal rabbitpox virus infection: A model of smallpox disease. Antiviral Res. 2015;117:115\u2013121. https:\/\/doi.org\/10.1016\/j.antiviral.2015.02.007","DOI":"10.1016\/j.antiviral.2015.02.007"},{"key":"ref72","doi-asserted-by":"crossref","unstructured":"Hutson CL, Kondas AV, Mauldin MR, et al. Pharmacokinetics and efficacy of a potential smallpox therapeutic brincidofovir in a lethal monkeypox virus animal model. mSphere. 2021;6(1):10\u2013128. https:\/\/doi.org\/10.1128\/mSphere.00126-21","DOI":"10.1128\/mSphere.00126-21"},{"key":"ref73","doi-asserted-by":"crossref","unstructured":"Grimley MS, Chemaly RF, Englund JA, et al. Brincidofovir for asymptomatic adenovirus viremia in pediatric and adult allogeneic hematopoietic cell transplant recipients: A randomized placebo-controlled phase II trial. Biol Blood Marrow Transplant. 2017;23(3):512\u2013521. https:\/\/doi.org\/10.1016\/j.bbmt.2016.12.621","DOI":"10.1016\/j.bbmt.2016.12.621"},{"key":"ref74","doi-asserted-by":"crossref","unstructured":"Marty FM, Winston DJ, Chemaly RF, et al. A randomized double-blind placebo-controlled phase 3 trial of oral brincidofovir for cytomegalovirus prophylaxis in allogeneic hematopoietic cell transplantation. Biol Blood Marrow Transplant. 2019;25(2):369\u2013381. https:\/\/doi.org\/10.1016\/j.bbmt.2018.09.038","DOI":"10.1016\/j.bbmt.2018.09.038"},{"key":"ref75","doi-asserted-by":"crossref","unstructured":"Yang G, Pevear DC, Davies MH, et al. An orally bioavailable antipoxvirus compound (ST-246) inhibits extracellular virus formation and protects mice from lethal orthopoxvirus challenge. J Virol. 2005;79(20):13139\u201313149. https:\/\/doi.org\/10.1128\/JVI.79.20.13139-13149.2005","DOI":"10.1128\/JVI.79.20.13139-13149.2005"},{"key":"ref76","doi-asserted-by":"crossref","unstructured":"Duraffour S, Snoeck R, De Vos R, et al. Activity of the anti-orthopoxvirus compound ST-246 against vaccinia, cowpox, and camelpox viruses in cell monolayers and organotypic raft cultures. Antivir Ther. 2007;12(8):1205\u20131216. https:\/\/doi.org\/10.1177\/135965350701200802","DOI":"10.1177\/135965350701200802"},{"key":"ref77","doi-asserted-by":"crossref","unstructured":"Jordan R, Chinsangaram J, Bolken TC, et al. Safety and pharmacokinetics of the antiorthopoxvirus compound ST-246 following repeat oral dosing in healthy adult subjects. Antimicrob Agents Chemother. 2010;54(6):2560\u20132566. https:\/\/doi.org\/10.1128\/AAC.01689-09","DOI":"10.1128\/AAC.01689-09"},{"key":"ref78","doi-asserted-by":"crossref","unstructured":"Russo AT, Grosenbach DW, Brasel TL, et al. Effects of treatment delay on efficacy of tecovirimat following lethal aerosol monkeypox virus challenge in cynomolgus macaques. J Infect Dis. 2018;218(9):1490\u20131499. https:\/\/doi.org\/10.1093\/infdis\/jiy326","DOI":"10.1093\/infdis\/jiy326"},{"key":"ref79","doi-asserted-by":"crossref","unstructured":"Rao AK. Monkeypox in a traveler returning from Nigeria: Dallas, Texas, July 2021. MMWR Morb Mortal Wkly Rep. 2022;71(14):509\u2013516.","DOI":"10.15585\/mmwr.mm7114a1"},{"key":"ref80","doi-asserted-by":"crossref","unstructured":"Wu JJ, Huang DB, Pang KR, Tyring SK. Vaccines and immunotherapies for the prevention of infectious diseases having cutaneous manifestations. J Am Acad Dermatol. 2004;50(4):495\u2013528. https:\/\/doi.org\/10.1016\/j.jaad.2003.12.003","DOI":"10.1016\/j.jaad.2003.12.003"},{"key":"ref81","doi-asserted-by":"crossref","unstructured":"Pettigrew ZJ, Kim M, Becker-Dreps S. Vaccines and immunization. In: Daaleman TP, Helton MR, editors. Chronic illness care: Principles and practice. Cham: Springer International Publishing; 2023, p. 155\u2013179.","DOI":"10.1007\/978-3-031-29171-5_12"},{"key":"ref82","doi-asserted-by":"crossref","unstructured":"Malik S, Asghar M, Waheed Y. Mitigation measures to control the expected mpox outbreak in a developing country: Pakistani scenario. Vaccines. 2023;11(5):502. https:\/\/doi.org\/10.3390\/vaccines11030502","DOI":"10.3390\/vaccines11030502"},{"key":"ref83","doi-asserted-by":"crossref","unstructured":"Yoshikawa T. Third-generation smallpox vaccine strain-based recombinant vaccines for viral hemorrhagic fevers. Vaccine. 2021;39(41):6174\u20136181. https:\/\/doi.org\/10.1016\/j.vaccine.2021.09.001","DOI":"10.1016\/j.vaccine.2021.09.001"},{"key":"ref84","doi-asserted-by":"crossref","unstructured":"Pischel L, Martini BA, Yu N, et al. Vaccine effectiveness of 3rd generation mpox vaccines against mpox and disease severity: A systematic review and meta-analysis. Vaccine. 2024;42(25):126053. https:\/\/doi.org\/10.1016\/j.vaccine.2024.06.021","DOI":"10.1016\/j.vaccine.2024.06.021"},{"key":"ref85","doi-asserted-by":"crossref","unstructured":"Saadh MJ, Ghadimkhani T, Soltani N, et al. Progress and prospects on vaccine development against monkeypox infection. Microb Pathog. 2023;180:106156. https:\/\/doi.org\/10.1016\/j.micpath.2023.106156","DOI":"10.1016\/j.micpath.2023.106156"},{"key":"ref86","doi-asserted-by":"crossref","unstructured":"Van Ewijk CE, Smit C, Bavalia R, et al. Acceptance and timeliness of post-exposure vaccination against mpox in high-risk contacts, Amsterdam, the Netherlands, May\u2013July 2022. Vaccine. 2023;41(47):6952\u20136959. https:\/\/doi.org\/10.1016\/j.vaccine.2023.10.013","DOI":"10.1016\/j.vaccine.2023.10.013"},{"key":"ref87","doi-asserted-by":"crossref","unstructured":"Rosenthal KS. Introduction to virology. In: Green LH, Goldman E, editors. Practical handbook of microbiology. CRC Press; 2021, p. 703\u2013722.","DOI":"10.1201\/9781003099277-51"},{"key":"ref88","doi-asserted-by":"crossref","unstructured":"Cices A, Prasad S, Akselrad M, et al. Mpox update: Clinical presentation, vaccination guidance, and management. Cutis. 2023;111(4):197\u2013202. https:\/\/doi.org\/10.12788\/cutis.0745","DOI":"10.12788\/cutis.0745"},{"key":"ref89","unstructured":"Food and Drug Administration. Fact sheet for healthcare providers administering vaccine: Emergency use authorization of JYNNEOS (smallpox and monkeypox vaccine live, non-replicating) for prevention of monkeypox disease in individuals determined to be at high risk for monkeypox infection. Silver Spring (MD): US Department of Health and Human Services, Food and Drug Administration; 2022."},{"key":"ref90","doi-asserted-by":"crossref","unstructured":"Aldhaeefi M, Rungkitwattanakul D, Unonu J, et al. The 2022 human monkeypox outbreak: Clinical review and management guidance. Am J Health Syst Pharm. 2023;80(2):44\u201352. https:\/\/doi.org\/10.1093\/ajhp\/zxac300","DOI":"10.1093\/ajhp\/zxac300"},{"key":"ref91","doi-asserted-by":"crossref","unstructured":"Rao AK. Use of JYNNEOS (smallpox and monkeypox vaccine live, non-replicating) for preexposure vaccination of persons at risk for occupational exposure to orthopoxviruses: Recommendations of the Advisory Committee on Immunization Practices\u2014United States, 2022. MMWR Morb Mortal Wkly Rep. 2022;71(22): 734\u2013742. https:\/\/doi.org\/10.15585\/mmwr.mm7122e1","DOI":"10.15585\/mmwr.mm7122e1"},{"key":"ref92","doi-asserted-by":"crossref","unstructured":"Smith YE, Bray M, Whitehouse CA, et al. Smallpox vaccine does not protect macaques with AIDS from a lethal monkeypox virus challenge. J Infect Dis. 2005;191(3):372\u2013381. https:\/\/doi.org\/10.1086\/427265","DOI":"10.1086\/427265"},{"key":"ref93","doi-asserted-by":"crossref","unstructured":"Saijo M, Ami Y, Suzaki Y, et al. LC16m8, a highly attenuated vaccinia virus vaccine lacking expression of the membrane protein B5R, protects monkeys from monkeypox. J Virol. 2006;80(11):5179\u20135188. https:\/\/doi.org\/10.1128\/JVI.02642-05","DOI":"10.1128\/JVI.02642-05"},{"key":"ref94","doi-asserted-by":"crossref","unstructured":"Garc\u00eda-Arriaza J, Esteban M, L\u00f3pez D. Modified vaccinia virus Ankara as a viral vector for vaccine candidates against chikungunya virus. Biomedicines. 2021;9(9):1122. https:\/\/doi.org\/10.3390\/biomedicines9091122","DOI":"10.3390\/biomedicines9091122"},{"key":"ref95","unstructured":"World Health Organization. Laboratory testing for the monkeypox virus: Interim guidance, 23 May 2022. Geneva: World Health Organization; 2022."},{"key":"ref96","doi-asserted-by":"crossref","unstructured":"Moschese D, Pozza G, Mileto D, et al. Isolation of viable monkeypox virus from anal and urethral swabs, Italy, May to July 2022. Euro Surveill. 2022;27(36):2200675. https:\/\/doi.org\/10.2807\/1560-7917.ES.2022.27.36.2200675","DOI":"10.2807\/1560-7917.ES.2022.27.36.2200675"},{"key":"ref97","doi-asserted-by":"crossref","unstructured":"Da Silva SJR, Kohl A, Pena L, Pardee K. Clinical and laboratory diagnosis of monkeypox (mpox): Current status and future directions. iScience. 2023;26(6):106759. https:\/\/doi.org\/10.1016\/j.isci.2023.106759","DOI":"10.1016\/j.isci.2023.106759"},{"key":"ref98","doi-asserted-by":"crossref","unstructured":"Lapa D, Carletti F, Mazzotta V, et al. Monkeypox virus isolation from a semen sample collected in the early phase of infection in a patient with prolonged seminal viral shedding. Lancet Infect Dis. 2022;22(12):1267\u20131269. https:\/\/doi.org\/10.1016\/S1473-3099(22)00513-8","DOI":"10.1016\/S1473-3099(22)00513-8"},{"key":"ref99","doi-asserted-by":"crossref","unstructured":"Ogunleye A, Dalhat M, Akinpelu A, et al. Mpox (monkeypox) risk and mortality associated with HIV infection: A national case\u2013control study in Nigeria. BMJ Glob Health. 2023;8(11):e013126. https:\/\/doi.org\/10.1136\/bmjgh-2023-013126","DOI":"10.1136\/bmjgh-2023-013126"},{"key":"ref100","unstructured":"Ladnyj ID, Ziegler P, Kima E. A human infection caused by monkeypox virus in Basankusu Territory, Democratic Republic of the Congo. Bull World Health Organ. 1972;46:593\u2013597."},{"key":"ref101","doi-asserted-by":"crossref","unstructured":"M\u00fcller M, Ingold-Heppner B, Stocker H, Heppner FL, Dittmayer C, Laue M. Electron microscopy images of monkeypox virus infection in a 24-year-old man. Lancet. 2022;400(10365):1618. https:\/\/doi.org\/10.1016\/S0140-6736(22)01969-9","DOI":"10.1016\/S0140-6736(22)01969-9"},{"key":"ref102","doi-asserted-by":"crossref","unstructured":"Kugelman JR, Johnston SC, Mulembakani PM, et al. Genomic variability of monkeypox virus among humans, Democratic Republic of the Congo. Emerg Infect Dis. 2014;20(2):232\u2013239. https:\/\/doi.org\/10.3201\/eid2002.130118","DOI":"10.3201\/eid2002.130118"},{"key":"ref103","doi-asserted-by":"crossref","unstructured":"Da Silva SJR, Pardee K, Balasuriya UBR, Pena L. Development and validation of a one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in patient samples from Brazil. Sci Rep. 2021;11:4111. https:\/\/doi.org\/10.1038\/s41598-021-83371-1","DOI":"10.1038\/s41598-021-83371-1"},{"key":"ref104","doi-asserted-by":"crossref","unstructured":"Petersen BW, Kabamba J, McCollum AM, et al. Vaccinating against monkeypox in the Democratic Republic of the Congo. Antiviral Res. 2019;162:171\u2013177. https:\/\/doi.org\/10.1016\/j.antiviral.2018.11.004","DOI":"10.1016\/j.antiviral.2018.11.004"},{"key":"ref105","doi-asserted-by":"crossref","unstructured":"Sood A, Sui Y, McDonough E, et al. Comparison of multiplexed immunofluorescence imaging to chromogenic immunohistochemistry of skin biomarkers in response to monkeypox virus infection. Viruses. 2020;12(8):787. https:\/\/doi.org\/10.3390\/v12080787","DOI":"10.3390\/v12080787"},{"key":"ref106","doi-asserted-by":"crossref","unstructured":"Johnston SC, Johnson JC, Stoniera SW, et al. Cytokine modulation correlates with severity of monkeypox disease in humans. J Clin Virol. 2015;63:42\u201345. https:\/\/doi.org\/10.1016\/j.jcv.2014.12.001","DOI":"10.1016\/j.jcv.2014.12.001"},{"key":"ref107","doi-asserted-by":"crossref","unstructured":"Costello V, Sowash M, Gaur A, et al. Imported monkeypox from international traveler, Maryland, USA, 2021. Emerg Infect Dis. 2022;28(5):1002\u20131005. https:\/\/doi.org\/10.3201\/eid2805.220292","DOI":"10.3201\/eid2805.220292"},{"key":"ref108","doi-asserted-by":"crossref","unstructured":"Rimoin AW, Mulembakani PM, Johnston SC, et al. Major increase in human monkeypox incidence 30 years after smallpox vaccination campaigns cease in the Democratic Republic of Congo. Proc Natl Acad Sci U S A. 2010;107(37):16262\u201316267. https:\/\/doi.org\/10.1073\/pnas.1005769107","DOI":"10.1073\/pnas.1005769107"},{"key":"ref109","doi-asserted-by":"crossref","unstructured":"Velavan TP, Meyer CG. Monkeypox 2022 outbreak: An update. Trop Med Int Health. 2022;27(7):604\u2013605. https:\/\/doi.org\/10.1111\/tmi.13785","DOI":"10.1111\/tmi.13785"},{"key":"ref110","doi-asserted-by":"crossref","unstructured":"I\u00f1igo Mart\u00ednez J, Gil Montalb\u00e1n E, Jim\u00e9nez Bueno S, et al. Viral dynamics in patients with monkeypox infection in Spain: A prospective cohort study. Lancet Infect Dis. 2023;23(4):445\u2013453.","DOI":"10.1016\/S1473-3099(22)00794-0"},{"key":"ref111","doi-asserted-by":"crossref","unstructured":"Magnus PV, Andersen E, Petersen K, Birch-Andersen A. A pox-like disease in cynomolgus monkeys. Acta Pathol Microbiol Scand. 1959;46(2):156\u2013165. https:\/\/doi.org\/10.1111\/j.1699-0463.1959.tb00328.x","DOI":"10.1111\/j.1699-0463.1959.tb00328.x"},{"key":"ref112","doi-asserted-by":"crossref","unstructured":"Miraglia del Giudice G, Della Polla G, Folcarelli L, Napoli A, Angelillo IF, Collaborative Working Group. Knowledge and attitudes of health care workers about monkeypox virus infection in Southern Italy. Front Public Health. 2023;11:1091267. https:\/\/doi.org\/10.3389\/fpubh.2023.1091267","DOI":"10.3389\/fpubh.2023.1091267"},{"key":"ref113","doi-asserted-by":"crossref","unstructured":"Kulesh DA, Loveless BM, Norwood D, et al. Monkeypox virus detection in rodents using real-time 3\u2019-minor groove binder TaqMan assays on the Roche LightCycler. Lab Invest. 2004;84:1200\u20131208. https:\/\/doi.org\/10.1038\/labinvest.3700143","DOI":"10.1038\/labinvest.3700143"},{"key":"ref114","doi-asserted-by":"crossref","unstructured":"Li D, Wilkins K, McCollum AM, et al. Evaluation of the GeneXpert for human monkeypox diagnosis. Am J Trop Med Hyg. 2017;96:405\u2013410. https:\/\/doi.org\/10.4269\/ajtmh.16-0567","DOI":"10.4269\/ajtmh.16-0567"},{"key":"ref115","unstructured":"Centers for Disease Control and Prevention (CDC). Test procedure: Monkeypox virus generic real-time PCR test. Atlanta, Georgia: CDC; 2022."},{"key":"ref116","doi-asserted-by":"crossref","unstructured":"Shchelkunov SN, Shcherbakov DN, Maksyutov RA, Gavrilova EV. Species-specific identification of variola, monkeypox, cowpox, and vaccinia viruses by multiplex real-time PCR assay. J Virol Methods. 2011;175(2):163\u2013169. https:\/\/doi.org\/10.1016\/j.jviromet.2011.05.002","DOI":"10.1016\/j.jviromet.2011.05.002"},{"key":"ref117","doi-asserted-by":"crossref","unstructured":"Huo S, Chen Y, Lu R, et al. Development of two multiplex real-time PCR assays for simultaneous detection and differentiation of monkeypox virus IIa, IIb, and I clades, and the B.1 lineage. Biosaf Health. 2022;4(5):392\u2013398. https:\/\/doi.org\/10.1016\/j.bsheal.2022.10.005","DOI":"10.1016\/j.bsheal.2022.10.005"},{"key":"ref118","doi-asserted-by":"crossref","unstructured":"Grant RJ, Baldwin CD, Nalca A, et al. Application of the Ibis-T5000 pan-Orthopoxvirus assay to quantitatively detect monkeypox viral loads in clinical specimens from macaques experimentally infected with aerosolized monkeypox virus. Am J Trop Med Hyg. 2010;82(2):318\u2013323. https:\/\/doi.org\/10.4269\/ajtmh.2010.09-0361","DOI":"10.4269\/ajtmh.2010.09-0361"},{"key":"ref119","doi-asserted-by":"crossref","unstructured":"Ribeiro da Silva SJR, Paiva MHS, Guedes DRD, et al. Development and validation of reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of ZIKV in mosquito samples from Brazil. Sci Rep. 2019;9:4494. https:\/\/doi.org\/10.1038\/s41598-019-40960-5","DOI":"10.1038\/s41598-019-40960-5"},{"key":"ref120","doi-asserted-by":"crossref","unstructured":"Ribeiro da Silva SJR, Pardee K, Pena L. Adaptive, diverse, and decentralized diagnostics are key to the future of outbreak response. BMC Biol. 2020;18:153. https:\/\/doi.org\/10.1186\/s12915-020-00891-4","DOI":"10.1186\/s12915-020-00891-4"},{"key":"ref121","doi-asserted-by":"crossref","unstructured":"Notomi T, Mori Y, Tomita N, Kanda H. Loop-mediated isothermal amplification (LAMP): Principle, features, and future prospects. J Microbiol. 2015;53(1):1\u20135. https:\/\/doi.org\/10.1007\/s12275-015-4656-9","DOI":"10.1007\/s12275-015-4656-9"},{"key":"ref122","doi-asserted-by":"crossref","unstructured":"Amaral C, Attunes W, Moe E, et al. A molecular test based on RT-LAMP for rapid, sensitive, and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples. Sci Rep. 2021;11:16430. https:\/\/doi.org\/10.1038\/s41598-021-95799-6","DOI":"10.1038\/s41598-021-95799-6"},{"key":"ref123","doi-asserted-by":"crossref","unstructured":"Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000;28(12):e63. https:\/\/doi.org\/10.1093\/nar\/28.12.e63","DOI":"10.1093\/nar\/28.12.e63"},{"key":"ref124","doi-asserted-by":"crossref","unstructured":"Nakazawa Y, Mauldin MR, Emerson GL, et al. A phylogeographic investigation of African monkeypox. Viruses. 2015;7(4):2168\u20132184. https:\/\/doi.org\/10.3390\/v7042168","DOI":"10.3390\/v7042168"},{"key":"ref125","doi-asserted-by":"crossref","unstructured":"Das A, Babiuk S, McIntosh MT. Development of a loop-mediated isothermal amplification assay for rapid detection of capripoxviruses. J Clin Microbiol. 2012;50(5):1613\u20131620. https:\/\/doi.org\/10.1128\/JCM.06796-11","DOI":"10.1128\/JCM.06796-11"},{"key":"ref126","doi-asserted-by":"crossref","unstructured":"Murray L, Edwards L, Tuppurainen ESM, et al. Detection of capripoxvirus DNA using a novel loop-mediated isothermal amplification assay. BMC Vet Res. 2013;9:90. https:\/\/doi.org\/10.1186\/1746-6148-9-90","DOI":"10.1186\/1746-6148-9-90"},{"key":"ref127","doi-asserted-by":"crossref","unstructured":"Mao L, Ying J, Selekon B, et al. Development and characterization of recombinase-based isothermal amplification assays (RPA\/RAA) for the rapid detection of monkeypox virus. Viruses. 2022;14(21):112. https:\/\/doi.org\/10.3390\/v14102112","DOI":"10.3390\/v14102112"},{"key":"ref128","doi-asserted-by":"crossref","unstructured":"Bhalla N, Jolly P, Formisano N, Estrela P. Introduction to biosensors. Essays Biochem. 2016;60(1):1\u20138. https:\/\/doi.org\/10.1042\/EBC20150001","DOI":"10.1042\/EBC20150001"},{"key":"ref129","doi-asserted-by":"crossref","unstructured":"Pardee K, Green AA, Takahashi MK, et al. Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell. 2016;165(5):1255\u20131266. https:\/\/doi.org\/10.1016\/j.cell.2016.04.059","DOI":"10.1016\/j.cell.2016.04.059"},{"key":"ref130","doi-asserted-by":"crossref","unstructured":"Jung JK, Alam KK, Verosloff MS, et al. Cell-free biosensors for rapid detection of water contaminants. Nat Biotechnol. 2020;38(12):1451\u20131459. https:\/\/doi.org\/10.1038\/s41587-020-0571-7","DOI":"10.1038\/s41587-020-0571-7"},{"key":"ref131","unstructured":"Xiang YU, Zhuohang WE, Shenggang SA, Rongguang ZH. Epidemiology and research progress in prevention and control of mpox. \u4e2d\u534e\u75be\u75c5\u63a7\u5236\u6742\u5fd7 [CJDCP]. 2024;28(10):1217\u20131222."},{"key":"ref132","doi-asserted-by":"crossref","unstructured":"Kannan SR, Sachdev S, Reddy AS, et al. Mutations in the monkeypox virus replication complex: Potential contributing factors to the 2022 outbreak. J Autoimmun. 2022;133:102928. https:\/\/doi.org\/10.1016\/j.jaut.2022.102928","DOI":"10.1016\/j.jaut.2022.102928"},{"key":"ref133","doi-asserted-by":"crossref","unstructured":"Mohapatra RK, Singh PK, Branda F, et al. Transmission dynamics, complications and mitigation strategies of the current mpox outbreak: A comprehensive review with bibliometric study. Rev Med Virol. 2024;34(3):e2541. https:\/\/doi.org\/10.1002\/rmv.2541","DOI":"10.1002\/rmv.2541"},{"key":"ref134","doi-asserted-by":"crossref","unstructured":"Mohanto S, Faiyazuddin M, Gholap AD, et al. Addressing the resurgence of global monkeypox (Mpox) through advanced drug delivery platforms. Travel Med Infect Dis. 2023;56:102636. https:\/\/doi.org\/10.1016\/j.tmaid.2023.102636","DOI":"10.1016\/j.tmaid.2023.102636"},{"key":"ref135","doi-asserted-by":"crossref","unstructured":"Youssef D, Abboud E, Kawtharani M, Zheim Z, Abou Arrage N, Youssef J. When a neglected tropical zoonotic disease emerges in non-endemic countries: Need to proactively fill the unveiled knowledge gaps towards human monkeypox among the Lebanese population. J Pharm Policy Pract. 2023;16(1):39. https:\/\/doi.org\/10.1186\/s40545-023-00544-1","DOI":"10.1186\/s40545-023-00544-1"},{"key":"ref136","doi-asserted-by":"crossref","unstructured":"Bourner J, Garcia-Gallo E, Mbrenga F, et al. Challenges in clinical diagnosis of Clade I Mpox: Highlighting the need for enhanced diagnostic approaches. PLoS Negl Trop Dis. 2024;18(6):e0012087. https:\/\/doi.org\/10.1371\/journal.pntd.0012087","DOI":"10.1371\/journal.pntd.0012087"},{"key":"ref137","doi-asserted-by":"crossref","unstructured":"Prins H, Coyer L, De Angelis S, Bluemel B, Cauchi D, Baka A. Evaluation of Mpox contact tracing activities and data collection in EU\/EEA countries during the 2022 multicountry outbreak in nonendemic countries. J Med Virol. 2024;96(1):e29352. https:\/\/doi.org\/10.1002\/jmv.29352","DOI":"10.1002\/jmv.29352"},{"key":"ref138","doi-asserted-by":"crossref","unstructured":"Lounis M, Riad A. Monkeypox (MPOX)-related knowledge and vaccination hesitancy in non-endemic countries: Concise literature review. 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