{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T16:45:08Z","timestamp":1725641108710},"reference-count":0,"publisher":"Medpharm Publications","issue":"2","license":[{"start":{"date-parts":[[1997,7,13]],"date-time":"1997-07-13T00:00:00Z","timestamp":868752000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. S. Afr. Vet. Assoc."],"abstract":"<jats:p>Clinical, virological and serological responses were evaluated in 10 pregnant mares after different challenge exposures to the asinine-94 strain of equine arteritis virus (EAV). The outcome of maternal infection on the progeny was also investigated. Mares were inoculated intranasally (n = 4), intramuscularly (n = 2), intravenously (n = 1), or contact-exposed (n = 3). All inoculated mares developed pyrexia, 5 showed mild clinical signs related to EAV infection and 2 remained asymptomatic. Viraemia was detected in all the inoculated animals and shedding of virus from the respiratory tract occurred in 6. Five mares were re-challenged intranasally 7 and 15 weeks after inoculation. Clinical signs of the disease in these mares were limited to mild conjunctivitis. After re-challenge, virus was recovered from buffy coat cultures of 2 mares 2-6 days after re-infection. EAV was not recovered from colostrum and milk samples during the 1st week post partum. All inoculated mares seroconverted to EAV 8-12 days post inoculation and also seroconverted after re-challenge. No clinical signs of EAV infection were observed in the 3 mares kept in close contact during the post-inoculation and re-challenge periods. Serum neutralising antibody to the virus was detected in 1 in-contact mare only, while a detectable concentration of specific IgG was found by ELISA in the colostrum of 1 of the other in-contact mares. Eight of the mares gave birth to clinically normal foals, although 1 was born prematurely. Shortly after birth, 7 foals developed fever and variable clinical signs; 5 foals became septicaemic and 3 of them died 2-5 days after birth, while the remaining 2 were euthanased at 1 month of age. EAV was not recovered from the placenta, from buffy coat fractions of blood collected from foals immediately after birth and 1-3 days later, or from a range of tissues taken from the 3 foals that died and 2 that were euthanased. Virus was not isolated from tissues collected from 1 mare and her foetus 3 weeks after this mare was re-challenged.<\/jats:p>","DOI":"10.4102\/jsava.v68i2.869","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T14:25:57Z","timestamp":1345818357000},"page":"49-54","source":"Crossref","is-referenced-by-count":2,"title":["Experimental exposure of pregnant mares to the asinine-94 strain of equine arteritis virus"],"prefix":"10.36303","volume":"68","author":[{"given":"J.T.","family":"Paweska","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.M.","family":"Henton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.J.","family":"Van der Lugt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22149","published-online":{"date-parts":[[1997,7,13]]},"container-title":["Journal of the South African Veterinary Association"],"original-title":[],"link":[{"URL":"https:\/\/journals.jsava.aosis.co.za\/index.php\/jsava\/article\/viewFile\/869\/843","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.jsava.aosis.co.za\/index.php\/jsava\/article\/viewFile\/869\/843","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T14:55:00Z","timestamp":1675090500000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.jsava.aosis.co.za\/index.php\/jsava\/article\/view\/869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,13]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[1997,7,13]]}},"URL":"https:\/\/doi.org\/10.4102\/jsava.v68i2.869","relation":{},"ISSN":["2224-9435","1019-9128"],"issn-type":[{"value":"2224-9435","type":"electronic"},{"value":"1019-9128","type":"print"}],"subject":[],"published":{"date-parts":[[1997,7,13]]}}}