{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T05:23:45Z","timestamp":1675142625509},"reference-count":0,"publisher":"Medpharm Publications","issue":"3","license":[{"start":{"date-parts":[[1999,7,12]],"date-time":"1999-07-12T00:00:00Z","timestamp":931737600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. S. Afr. Vet. Assoc."],"abstract":"<jats:p>A Holstein cow of high genetic merit, in late lactation (205 days) and diagnosed with salpingitis (after 4 infertile services and veterinary consultation), was subjected to 1 trans-vaginal oocyte collection attempt, prior to slaughter.Of an estimated 10 follicles punctured, a total of 4 cumulus-oocyte complexes were retrieved. These were matured in vitro in a maturation medium for 24 hours. After 24 hours maturation, the oocytes were fertilised in vitro with Percoll-processed frozen \/ thawed imported semen, of the owner's choice. Fertilisation was achieved in a modified Tyrode's medium. At 18 hours post-insemination, the presumptive zygotes were transferred into culture in vitro in Charles Rosenkran's aminoacid medium and supplemented on Day 4 post-insemination with 10 % foetal calf serum. All in vitro procedures were conducted in 50 m\u2113 medium droplets, under oil, in a humidified incubator at 38.5 oC in 5% CO2 in air. Three of the potential zygotes cleaved and, by Day 7 of culture, these had developed to the morula stage. The embryos were frozen in 1.5 M ethylene glycol and later transferred non-surgically to synchronised Holstein recipient heifers. One morula resulted in the only pregnancy and subsequent birth of a healthy heifer calf. An independent commercial company confirmed parentage through standard bloodtyping assays. The genetic salvage of oocytes, for in vitro production of embryos, has potential benefits to the producer.<\/jats:p>","DOI":"10.4102\/jsava.v70i3.773","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T14:25:57Z","timestamp":1345818357000},"page":"130-131","source":"Crossref","is-referenced-by-count":1,"title":["Trans-vaginal oocyte retrieval and subsequent in vitro production of embryos from a cow involuntarily culled : case report"],"prefix":"10.36303","volume":"70","author":[{"given":"D.G.","family":"Shaw","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.M.","family":"Bowles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Raja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.W.","family":"Lishman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"22149","published-online":{"date-parts":[[1999,7,12]]},"container-title":["Journal of the South African Veterinary Association"],"original-title":[],"link":[{"URL":"https:\/\/journals.jsava.aosis.co.za\/index.php\/jsava\/article\/viewFile\/773\/746","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.jsava.aosis.co.za\/index.php\/jsava\/article\/viewFile\/773\/746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T14:54:24Z","timestamp":1675090464000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.jsava.aosis.co.za\/index.php\/jsava\/article\/view\/773"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7,12]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[1999,7,12]]}},"URL":"https:\/\/doi.org\/10.4102\/jsava.v70i3.773","relation":{},"ISSN":["2224-9435","1019-9128"],"issn-type":[{"value":"2224-9435","type":"electronic"},{"value":"1019-9128","type":"print"}],"subject":[],"published":{"date-parts":[[1999,7,12]]}}}