{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,3]],"date-time":"2022-04-03T17:04:32Z","timestamp":1649005472573},"reference-count":0,"publisher":"AOSIS","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Onderstepoort J Vet Res"],"abstract":"<jats:p>The occurrence of cross-resistance among  melarsoprol-resistant Trypanosoma brucei rhodesiense isolates was  investigated in this study. The isolates, T. b. rhodesiense KETRI 237,  2538, 1992, 2709, 2694 and 3530, had been obtained from sleeping sickness  patients in Kenya and Uganda between 1960 and 1985. Five groups consisting of  six mice each were inoculated intraperitoneally with 105 parasites of each  isolate, and 24 h later treated with either melarsoprol, homidium chloride,  diminazene aceturate or isometamidium chloride. The control group comprised  infected but untreated mice. The mice were monitored for cure for a period of 60  days post-treatment. The mean prepatent period in the control mice was 5 days  while the mean survival period was 22 days. Five of the stabilates, KETRI 237,  2538, 2709, 2694, and 3530, were confirmed to be melarsoprol resistant.  Cross-resistance was observed, with the majority of the isolates being resistant  to homidium chloride (5\/6) and diminazene aceturate (5\/6), but all were  sensitive to isometamidium chloride (6\/6). However T. b. rhodesiense KETRI 1992, which was previously considered as melarsoprol resistant, was  sensitive to all the drugs tested. In conclusion, our study has revealed the  existence of cross-resistance among the melarsoprol resistant isolates which  could only be cured by isometamidium.<\/jats:p>","DOI":"10.4102\/ojvr.v74i1.135","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T07:22:00Z","timestamp":1346052120000},"source":"Crossref","is-referenced-by-count":0,"title":["Occurrence of multiple drug resistance in &lt;i&gt;Trypanosoma brucei rhodesiense&lt;\/i&gt; isolated from sleeping sickness patients"],"prefix":"10.4102","volume":"74","author":[{"given":"N.","family":"Maina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Kagira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2580","published-online":{"date-parts":[[2007,9,13]]},"container-title":["Onderstepoort J Vet Res"],"original-title":[],"link":[{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/135\/130","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T11:20:59Z","timestamp":1496316059000},"score":1,"resource":{"primary":{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/view\/135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9,13]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2007,9,13]]}},"URL":"https:\/\/doi.org\/10.4102\/ojvr.v74i1.135","relation":{},"ISSN":["2219-0635","0030-2465"],"issn-type":[{"value":"2219-0635","type":"electronic"},{"value":"0030-2465","type":"print"}],"subject":[],"published":{"date-parts":[[2007,9,13]]}}}