{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T08:53:17Z","timestamp":1666342397794},"reference-count":0,"publisher":"AOSIS","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Onderstepoort J Vet Res"],"abstract":"<jats:p>Accurate measurements of serum aminotransferase (ALT)  activity in dogs relies on the endogenous pro-enzyme pyridoxal 5-phosphate  (P5P). The purpose of this study was to determine whether the exclusion of P5P  from the analytical method causes an underestimation of serum ALT activity in  dogs suffering from babesiosis and in those manifesting evidence of  hepatocellular damage, and to determine if anorexia causes sufficient P5P  depletion to affect in vitro serum ALT activity. One-hundred-and-twenty  healthy control dogs and 105 Babesia-infected dogs were included in the study.  Two methods for ALT measurement were used: Method 1 included P5P, and Method 2  excluded P5P from the reaction mixture. Higher serum ALT activity was measured  with Method 1 in the Babesia-infected dogs (P &lt; 0.001), as well as in  14 dogs with suspected hepatocellular damage (P = 0.03). Duration of  anorexia had no effect, irrespective of the method used. Although inclusion of  P5P to the reaction mixture consistently resulted in higher measured serum ALT  activity, the differences were too small to have led to incorrect diagnoses in  the Babesia-infected dogs suspected of liver disease.<\/jats:p>","DOI":"10.4102\/ojvr.v76i3.38","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T07:21:52Z","timestamp":1346052112000},"source":"Crossref","is-referenced-by-count":1,"title":["The effect of pyridoxal-5-phosphate on serum alanine aminotransferase activity in dogs suffering from canine babesiosis"],"prefix":"10.4102","volume":"76","author":[{"given":"E.C.","family":"Myburgh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Goddard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2580","published-online":{"date-parts":[[2009,9,9]]},"container-title":["Onderstepoort J Vet Res"],"original-title":[],"link":[{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/38\/33","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T11:20:12Z","timestamp":1496316012000},"score":1,"resource":{"primary":{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/view\/38"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,9]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2009,9,9]]}},"URL":"https:\/\/doi.org\/10.4102\/ojvr.v76i3.38","relation":{},"ISSN":["2219-0635","0030-2465"],"issn-type":[{"value":"2219-0635","type":"electronic"},{"value":"0030-2465","type":"print"}],"subject":[],"published":{"date-parts":[[2009,9,9]]}}}