{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:30:44Z","timestamp":1765827044568},"reference-count":0,"publisher":"AOSIS","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Onderstepoort J Vet Res"],"abstract":"<jats:p>Of 1 346 faecal samples from the Chikwawa and Thyolo  districts of Malawi, analysed for the presence of Cryptosporidium oocysts  between October 2001 and May 2003, 61.3 % were from cattle (29.8 % of these were  from calves &lt; 6 months old). Cryptosporidium oocysts were detected  during all three seasons studied in Chikwawa and Thyolo. In Chikwawa, 13.6 % of  adult cattle and 11.7 % of calves were infected, compared to 28.9 % of adult  cattle and 36.7 % of calves in Thyolo. Dependent on season, between 7.8 % and  37.7 % (Chikwawa) and 16.7 % and 39.3 % (Thyolo) of cattle samples contained  oocysts. In Chikwawa, the highest percentage of infections occurred in the cool  season, whereas in Thyolo, the highest percentage of infections occurred in the  dry season. Faecal samples from goats [n = 225], pigs [n = 92],  sheep [n = 6]), rabbits, guinea pigs, chickens, ducks, turkeys, doves and  guinea fowls were also analysed. Up to 5.6 % of goat samples contained oocysts  in Chikwawa, compared to between 16.7 % and 39.3 % in Thyolo. Again, in  Chikwawa, the highest percentage of infections occurred in the cool season and  the lowest in the rainy season, whereas, in Thyolo, the highest percentage of  infections occurred in the dry season and the lowest in the cool season. In  pigs, more infections were detected in the dry season in Chikwawa, but  infections in the cool season were similar (17.7 %), whereas in Thyolo,  infections occurred in all three seasons (17.9 % in the rainy season, 25 % in  the cool season and 60 % in the dry season). Often diarrhoeic, oocyst positive  cattle faecal samples collected from Chikwawa and subjected to PCR-RFLP, four  oocyst positive samples (two from heifers, one from a cow and one unknown) were  amplified at an 18S rRNA and Cryptosporidium oocyst wall protein (COWP)  loci. RFLP of the 18S rRNA locus indicated that Cryptosporidium parvum,  Cryptosporidium hominis, Cryptosporidium bovis and \/ or Cryptosporidium  ryanae DNA, or a mixture of them was present. Cryptosporidium parvum DNA was identified in one sample that amplified at the COWP locus,  indicating the presence of the major zoonotic Cryptosporidium species in  Malawi.<\/jats:p>","DOI":"10.4102\/ojvr.v76i4.19","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T07:21:52Z","timestamp":1346052112000},"source":"Crossref","is-referenced-by-count":10,"title":["&lt;i&gt;Cryptosporidium&lt;\/i&gt; infection in non-human hosts in Malawi"],"prefix":"10.4102","volume":"76","author":[{"given":"Z.","family":"Banda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosely A.B.","family":"Nichols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.M.","family":"Grimason","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.V.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2580","published-online":{"date-parts":[[2009,9,9]]},"container-title":["Onderstepoort J Vet Res"],"original-title":[],"link":[{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/19\/17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T11:20:04Z","timestamp":1496316004000},"score":1,"resource":{"primary":{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/view\/19"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,9]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,9,9]]}},"URL":"https:\/\/doi.org\/10.4102\/ojvr.v76i4.19","relation":{},"ISSN":["2219-0635","0030-2465"],"issn-type":[{"value":"2219-0635","type":"electronic"},{"value":"0030-2465","type":"print"}],"subject":[],"published":{"date-parts":[[2009,9,9]]}}}