{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T19:59:38Z","timestamp":1723060778957},"reference-count":0,"publisher":"AOSIS","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Onderstepoort J Vet Res"],"abstract":"<jats:p>The Mkuze Game Reserve (MGR), in north-eastern  KwaZulu-Natal Province, South Africa is an African swine fever virus (ASF)  controlled area. In a survey conducted in 1978, ASF prevalence in warthogs and  Ornithodoros ticks in MGR was determined to be 2 % and 0.06 %,  respectively. These values, acknowledged as being unusually low compared to  other East and southern African ASF-positive sylvatic-cycle host populations,  have not been assessed since. The availability of a sensitive PCR-based virus  detection method, developed specifically for the sylvatic tampan host, prompted  a re-evaluation of ASF virus (ASFV) prevalence in MGR ticks. Of the 98 warthog  burrows inspected for Ornithodoros presence, 59 (60.2 %) were found to  contain tampans and tick sampling was significantly male-biased. Whilst gender  sampling-bias is not unusual, the 27 % increase in infestation rate of warthog  burrows since the 1978 survey is noteworthy as it anticipates a concomitant  increase in ASFV prevalence, particularly in light of the high proportion (75 %)  of adult ticks sampled. However, despite DNA integrity being confirmed by  internal control amplification of the host 16S gene, PCR screening failed to  detect ASFV. These results suggest that ASFV has either disappeared from MGR or  if present, is localized, occurring at exceptionally low levels. Further  extensive surveys are required to establish the ASFV status of sylvatic hosts in  this controlled area.<\/jats:p>","DOI":"10.4102\/ojvr.v76i4.22","type":"journal-article","created":{"date-parts":[[2012,8,27]],"date-time":"2012-08-27T07:21:52Z","timestamp":1346052112000},"source":"Crossref","is-referenced-by-count":16,"title":["Molecular monitoring of African swine fever virus using surveys targeted at adult Ornithodoros ticks : a re-evaluation of Mkuze Game Reserve, South Africa"],"prefix":"10.4102","volume":"76","author":[{"given":"L.F.","family":"Arnot","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.T.","family":"Du Toit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.D.S.","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"2580","published-online":{"date-parts":[[2009,9,9]]},"container-title":["Onderstepoort J Vet Res"],"original-title":[],"link":[{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/22\/19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T11:20:05Z","timestamp":1496316005000},"score":1,"resource":{"primary":{"URL":"http:\/\/ojvr.org\/index.php\/ojvr\/article\/view\/22"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,9]]},"references-count":0,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2009,9,9]]}},"URL":"https:\/\/doi.org\/10.4102\/ojvr.v76i4.22","relation":{},"ISSN":["2219-0635","0030-2465"],"issn-type":[{"value":"2219-0635","type":"electronic"},{"value":"0030-2465","type":"print"}],"subject":[],"published":{"date-parts":[[2009,9,9]]}}}