{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:06:00Z","timestamp":1785402360176,"version":"3.56.0"},"reference-count":26,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Paratuberculosis (Johne\u2019s disease) has devastating outcomes on ruminant health and impacts on national and international trade. The current work assessed the diagnostic value of the VersaTREK automated liquid culture system in isolating Mycobacterium avium subspecies paratuberculosis (MAP) from faecal and intestinal tissue samples from ovine under South African conditions and compared it with the current method of choice, histopathological examination. Intestinal tissue and faecal samples from 111 sheep (including complete set from 104 slaughter sheep from flocks with a history of MAP infection as well as incomplete sample sets from 7 sheep) were analysed using the liquid culture method. One set of tissues was subjected to histopathological examination. Deoxyribonucleic acid (DNA) extracted from culture isolates was subjected to polymerase chain reaction (PCR) amplification using primers that target the IS900 regions of the MAP for species verification. Overall, the VersaTREK automated liquid culture in combination with IS900 PCR showed a comparable level of detection in tissues (12.6%) as histopathology (13.5%), but the detection rate for faecal samples was lower than for tissues (10.8%). A combination of histopathology and faecal culture increased the detection rate from 13.5% (n = 14\/104) and 9.6% (n = 10\/104), respectively, to 15.4% (n = 16\/104).\n\nContribution: Our findings highlight the diagnostic utility of the VersaTREK automated liquid culture system in detecting MAP in ovine samples collected both ante and postmortem. However, an inhibitory effect on the MAP isolation rate observed when the antibiotic cocktail was added to the culture medium warrants further investigation. The outcome of the study is beneficial in guiding the strategic planning of the nationwide control programme.<\/jats:p>","DOI":"10.4102\/ojvr.v92i1.2159","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T07:12:41Z","timestamp":1739257961000},"update-policy":"https:\/\/doi.org\/10.4102\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Are there benefits of culture-based detection of Mycobacterium avium spp paratuberculosis over histopathology?"],"prefix":"10.4102","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2791-3243","authenticated-orcid":false,"given":"Motlatso T.","family":"Hlokwe","sequence":"first","affiliation":[{"name":"ARC-Onderstepoort Veterinary Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8100-2195","authenticated-orcid":false,"given":"Nomawethu S.","family":"Masina","sequence":"additional","affiliation":[{"name":"ARC-Onderstepoort Veterinary Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4791-4638","authenticated-orcid":false,"given":"Boitumelo","family":"Letsoko","sequence":"additional","affiliation":[{"name":"ARC-Onderstepoort Veterinary Research"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3977-9766","authenticated-orcid":false,"given":"Sewellyn C.","family":"Davey","sequence":"additional","affiliation":[{"name":"State Veterinarian Malmesbury"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9240-2653","authenticated-orcid":false,"given":"Anita L.","family":"Michel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00g0p6g84","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Pretoria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2580","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"Biet, F. & Boschiroli, M.L., 2014, \u2018Non-tuberculous mycobacterial infections of veterinary relevance\u2019, Research in Veterinary Science 97, S69\u2013S77. https:\/\/doi.org\/10.1016\/j.rvsc.2014.08.007","DOI":"10.1016\/j.rvsc.2014.08.007"},{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Bradner, L., Robbe-Austerman, S., Beitz, D.C. & Stabel, J.R., 2013, \u2018Optimization of hexadecylpyridinium chloride decontamination for culture of Mycobacterium avium subsp. paratuberculosis from milk\u2019, Journal of Clinical Microbiology 51(5), 1575\u20131577. https:\/\/doi.org\/10.1128\/JCM.00333-13","DOI":"10.1128\/JCM.00333-13"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Coelho, A.C., Coelho, A.M., Garc\u00cdA-Diez, J., Pires, M.A. & Pinto, M.L., 2017, 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(eds.), First Asia Dairy Goat Conference, Corus Hotel Kuala Lumpur, Universiti Putra Malaysia and The Food and Agricultural Organization of the United Nations, Malaysia, April 9\u201312, 2012, vol. 9, p. 170."}],"container-title":["Onderstepoort Journal of Veterinary Research"],"original-title":[],"link":[{"URL":"https:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/2159\/2693","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/2159\/2694","content-type":"application\/epub+zip","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/2159\/2695","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojvr.org\/index.php\/ojvr\/article\/viewFile\/2159\/2696","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojvr.org\/index.php\/ojvr\/article\/view\/2159\/2693","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T04:46:17Z","timestamp":1748580377000},"score":1,"resource":{"primary":{"URL":"https:\/\/ojvr.org\/index.php\/ojvr\/article\/view\/2159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,11]]},"references-count":26,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1,1]]}},"URL":"https:\/\/doi.org\/10.4102\/ojvr.v92i1.2159","relation":{},"ISSN":["0030-2465","2219-0635"],"issn-type":[{"value":"0030-2465","type":"print"},{"value":"2219-0635","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,11]]},"article-number":"a2159"}}