{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:41:32Z","timestamp":1785404492644,"version":"3.56.0"},"reference-count":29,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T00:00:00Z","timestamp":1741305600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The widespread use of parabens has led to their accumulation in aquatic environments. This study examined the effects of methylparaben on rainbow trout, dividing 96 fish into control and treatment groups (1 mg\/L, 5 mg\/L, and 8 mg\/L). Results showed dose-dependent weight loss, altered hepatosomatic indices, increased serum urea, uric acid, and Malondialdehyde (MDA) levels, and decreased Glutathione Peroxidase (GSH-Px) activity. Histopathological analysis revealed liver and kidney abnormalities in treated groups, including hepatocyte degeneration, proliferation in the bile duct, glomerular atrophy, reduced haematopoietic tissue, increased melanomacrophage centres, necrosis and fibrosis.\n\nContribution: These findings highlight methylparaben\u2019s toxic effects, emphasising the need for stricter regulations and further research to safeguard aquatic ecosystems and understand its impact on aquatic organisms.<\/jats:p>","DOI":"10.4102\/ojvr.v92i1.2200","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T07:20:56Z","timestamp":1741332056000},"update-policy":"https:\/\/doi.org\/10.4102\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["Investigating methylparaben\u2019s oxidative stress effects on rainbow trout blood, liver, and kidney toxicity"],"prefix":"10.4102","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2581-0563","authenticated-orcid":false,"given":"Mert","family":"Calisir","sequence":"first","affiliation":[{"name":"Iskenderun Technical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5861-8538","authenticated-orcid":false,"given":"Gokhan","family":"Nur","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/052nzqz14","id-type":"ROR","asserted-by":"publisher"}],"name":"\u0130skenderun Technical University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0408-9690","authenticated-orcid":false,"given":"Emrah","family":"Caylak","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/026b8w395","id-type":"ROR","asserted-by":"publisher"}],"name":"Girne American 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2010, \u2018Vitellogenin induction and histo-metabolic changes following exposure of Cyprinus carpio to methylparaben\u2019, Asian-Australasian Journal of Animal Sciences 23, 1557\u20131565. https:\/\/doi.org\/10.5713\/ajas.2010.10118","DOI":"10.5713\/ajas.2010.10118"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Bedoux, G., Roig, B., Thomas, O., Dupont, V. & Le Bot, B., 2012, \u2018Occurrence and toxicity of antimicrobial triclosan and by-products in the environment\u2019, Environmental Science and Pollution Research International 19(4), 1044\u20131065. https:\/\/doi.org\/10.1007\/s11356-011-0632-z","DOI":"10.1007\/s11356-011-0632-z"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Bernet, D., Schmidt, H., Meier, W., Brkhardt-Holm, P. & Wahli, T., 1999, \u2018Histopathology in fish: Proposal for a protocol to assess aquatic pollution\u2019, Journal of Fish Diseases 22(1), 25\u201334. https:\/\/doi.org\/10.1046\/j.1365-2761.1999.00134.x","DOI":"10.1046\/j.1365-2761.1999.00134.x"},{"key":"ref5","unstructured":"Coskun, O.F., Aydin, D. & Duman, F., 2016, \u2018Comparison of some blood parameters of rainbow trout (Oncorhynchus mykiss) living in running and still water\u2019, Iranian Journal of Fisheries Sciences 15(1), 497\u2013507."},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Dasmahapatra, A.K., Chatterjee, J. & Tchounwou, P.B., 2024, \u2018A systematic review of the toxic potential of parabens in fish\u2019, Frontiers in Toxicology 6, 1399467. https:\/\/doi.org\/10.3389\/ftox.2024.1399467","DOI":"10.3389\/ftox.2024.1399467"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"De Carvalho Penha, L.C., Rola, R.C., Da Silva Junior, F.M. & De Martinez Gaspar Martins, C., 2021, \u2018Toxicity and sublethal effects of methylparaben on zebrafish (Danio rerio) larvae and adults\u2019, Environmental Science and Pollution Research 28, 45534\u201345544. https:\/\/doi.org\/10.1007\/s11356-021-12800-5","DOI":"10.1007\/s11356-021-12800-5"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Deveci, H.A., Karapehlivan, M., Kaya, I., Kukurt, A. & Alpay, M., 2015, \u2018Protective role of caffeic acid phenethyl ester against chlorpyrifos-ethyl acute poisoning\u2019, Ankara Universitesi Veteriner Fakultesi Dergisi 62(4), 255\u2013260. https:\/\/doi.org\/10.1501\/Vetfak_0000002689","DOI":"10.1501\/Vetfak_0000002689"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Dogan, D., Deveci, H.A. & Nur, G., 2021, \u2018Manifestations of oxidative stress and liver injury in clothianidin exposed Oncorhynchus mykiss\u2019, Toxicology Research 10(3), 501\u2013510. https:\/\/doi.org\/10.1093\/toxres\/tfab061","DOI":"10.1093\/toxres\/tfab027"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Dogan, D., Nur, G. & Deveci, H.A., 2022, \u2018Tissue-specific toxicity of clothianidin on rainbow trout (Oncorhynchus mykiss)\u2019, Drug and Chemical Toxicology 45(4), 1851\u20131861. https:\/\/doi.org\/10.1080\/01480545.2021.1892128","DOI":"10.1080\/01480545.2021.1892128"},{"key":"ref11","unstructured":"EPA, 2008, Estimations Programs Interface (EPI) Suite for Microsoft Windows\u00ae, v 4.10, United States Environmental Protection Agency, Washington, DC, viewed 12 September 2024, from http:\/\/www.epa.gov."},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Hu, C., Sun, B., Tang, L., Liu, M., Huang, Z., Zhou, X. et al., 2022, \u2018Hepatotoxicity caused by methylparaben in adult zebrafish\u2019, Aquatic Toxicology (Amsterdam, Netherlands) 250, 106255. https:\/\/doi.org\/10.1016\/j.aquatox.2022.106255","DOI":"10.1016\/j.aquatox.2022.106255"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Huang, L., Xu, J., Jia, K., Wu, Y., Yuan, W., Liao, Z. et al., 2024, \u2018Butylparaben induced zebrafish (Danio rerio) kidney injury by down-regulating the PI3K-AKT pathway\u2019, Journal of Hazardous Materials 470, 134129. https:\/\/doi.org\/10.1016\/j.jhazmat.2024.134129","DOI":"10.1016\/j.jhazmat.2024.134129"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Inkaya, E.N. & Barlas, N., 2022, \u2018Investigation of combined effects of propylparaben and methylparaben on the hypothalamic-pituitary-adrenal axis in male rats\u2019, Toxicology and Industrial Health 38(10), 687\u2013701. https:\/\/doi.org\/10.1177\/07482337221117652","DOI":"10.1177\/07482337221117652"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Jeong, J., Xue, K.J., Park, K., Kannan, H.B. & Moon, T., 2019, \u2018Tissue-specific accumulation and body burden of parabens and their metabolites in small cetaceans\u2019, Environmental Science & Technology 53, 475\u2013481. https:\/\/doi.org\/10.1021\/acs.est.8b04670","DOI":"10.1021\/acs.est.8b04670"},{"key":"ref16","unstructured":"Kelestemur, G.T. & Ozdemir, Y., 2010, \u2018Effects of transplantation on some blood parameter values of rainbow trout (Oncorhynchus mykiss)\u2019, Suleyman Demirel University Faculty of Arts and Science Journal of Science 5(2), 187\u2013193."},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Li, Z., Jia, K., Chen, X., Guo, J., Zheng, Z., Chen, W. et al., 2023, \u2018Exposure to butylparaben induces craniofacial bone developmental toxicity in zebrafish (Danio rerio) embryos\u2019, Ecotoxicology and Environmental Safety 265, 115523. https:\/\/doi.org\/10.1016\/j.ecoenv.2023.115523","DOI":"10.1016\/j.ecoenv.2023.115523"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Lincho, J., Martins, R.C. & Gomes, J., 2021, \u2018Paraben compounds \u2013 Part I: An overview of their characteristics, detection, and impacts\u2019, Applied Sciences 11(5), 1\u201338. https:\/\/doi.org\/10.3390\/app11052307","DOI":"10.3390\/app11052307"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Ma, Y., Li, Y., Song, X., Yang, T., Wang, H., Liang, Y. et al., 2023, \u2018Endocrine disruption of propylparaben in the male mosquitofish (Gambusia affinis): Tissue injuries and abnormal gene expressions of hypothalamic-pituitary-gonadal-liver axis\u2019, International Journal of Environmental Research and Public Health 20(4), 3557. https:\/\/doi.org\/10.3390\/ijerph20043557","DOI":"10.3390\/ijerph20043557"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Martins, M.F., Costa, P.G. & Bianchini, A., 2023, \u2018Bioaccumulation and potential impacts of persistent organic pollutants and contaminants of emerging concern in guitarfishes and angel sharks from Southeastern Brazil\u2019, Science of the Total Environment 893, 164873. https:\/\/doi.org\/10.1016\/j.scitotenv.2023.164873","DOI":"10.1016\/j.scitotenv.2023.164873"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Nur, G. & Deveci, H.A., 2018, \u2018Histopathological and biochemical responses to the oxidative stress induced by glyphosate-based herbicides in the rainbow trout (Oncorhynchus mykiss)\u2019, Journal of Cellular Neuroscience and Oxidative Stress 10(1), 656\u2013665. https:\/\/doi.org\/10.37212\/jcnos.418666","DOI":"10.37212\/jcnos.418666"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Pereira, A.R., Sim\u00f5es, M. & Gomes, I.B., 2023, \u2018Parabens are environmental contaminants of aquatic systems that affect water quality and microbial dynamics\u2019, Science of the Total Environment 905, 167332. https:\/\/doi.org\/10.1016\/j.scitotenv.2023.167332","DOI":"10.1016\/j.scitotenv.2023.167332"},{"key":"ref23","unstructured":"Presnell, J.K. & Schreibman, M.P., 1997, Humason\u2019s animal tissue techniques, 5th edn., pp. 269\u2013271, Johns Hopkins University Press, London."},{"key":"ref24","unstructured":"Pirinc, B. & Turkoglu, S., 2021, \u2018Investigation of the effects of ethylparaben and methylparaben on the longevity and fecundity of Caenorhabditis elegans\u2019, Cumhuriyet University Faculty of Science Journal 37(4), 371\u2013390."},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Ross, L. & Ross, B., 2008, Anaesthetic and sedative techniques for aquatic animals, ed. 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