{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:30:18Z","timestamp":1792053018370,"version":"4.4.0"},"reference-count":40,"publisher":"AOSIS","issue":"1","license":[{"start":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T00:00:00Z","timestamp":1704412800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Background: Non-tuberculous mycobacteria (NTM) management comprises prolonged therapy that includes macrolides. Non-tuberculous mycobacteria can cause disease in patients with predisposing conditions such as HIV and structural lung disease. Local data on NTM disease and macrolide resistance are scarce, and routine antimicrobial susceptibility testing is currently not performed for NTM in South Africa.\n\nObjectives: This study aims to characterise NTM isolated at Tshepong National Health Laboratory Service (NHLS) according to species and antimicrobial susceptibility pattern.\n\nMethod: A retrospective data analysis of NTM isolates from Tshepong NHLS was performed from January to June 2020. GenoType\u00ae NTM-DR was performed on selected isolates where the assay can confirm the species and determine resistance to macrolides and aminoglycosides.\n\nResults: Of the 194 collected NTM isolates, 183 were included in the study. Patients\u2019 ages ranged from 1 day to 81 years (median 36 years). The most common specimen was sputum (84.7%), followed by gastric aspirate (6.6%). The most common NTM isolated were Mycobacterium (M.) intracellulare (67.6%), M. fortuitum (12.6%), M. species (4.3%), M. kansasii (3.9%), and M. scrofulaceum (3.9%). Macrolide resistance occurred in 2.8% of tested isolates; no aminoglycoside resistance was detected. Although most isolates were from males (62.3%), resistance was observed only in females.\n\nConclusion: M. intracellulare predominated, with only two M. intracellulare and two M. abscessus isolates showing macrolide resistance; aminoglycoside resistance was absent.\n\nContribution: This study highlights the need for increased awareness of NTM, regular nationwide NTM surveillance, and monitoring of resistance trends to guide future patient management and ensure good treatment outcomes.<\/jats:p>","DOI":"10.4102\/sajid.v39i1.525","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T04:46:20Z","timestamp":1704429980000},"update-policy":"https:\/\/doi.org\/10.4102\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterisation and antimicrobial susceptibility pattern of non-tuberculous mycobacteria"],"prefix":"10.4102","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3294-3981","authenticated-orcid":false,"given":"Abraham J.","family":"le Roux","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009xwd568","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Free State"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3892-8499","authenticated-orcid":false,"given":"Anneke","family":"van der Spoel van Dijk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009xwd568","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Free State"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4566-0742","authenticated-orcid":false,"given":"Motlatji R.B.","family":"Maloba","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009xwd568","id-type":"ROR","asserted-by":"publisher"}],"name":"University of the Free State"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"2580","published-online":{"date-parts":[[2024,1,5]]},"reference":[{"key":"ref0","doi-asserted-by":"crossref","unstructured":"Matsumoto Y, Kinjo T, Motooka D, et al. 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Antimicrob Agents Chemother. 2015;59(11):7153\u20137155. https:\/\/doi.org\/10.1128\/AAC.01027-15","DOI":"10.1128\/AAC.01027-15"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Lee SH, Yoo HK, Kim SH, et al. The drug resistance profile of Mycobacterium abscessus group strains from Korea. Ann Lab Med. 2014;34(1):31\u201337. https:\/\/doi.org\/10.3343\/alm.2014.34.1.31","DOI":"10.3343\/alm.2014.34.1.31"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Mougari F, Loiseau J, Veziris N, et al. Evaluation of the new GenoType NTM-DR kit for the molecular detection of antimicrobial resistance in non-tuberculous mycobacteria. J Antimicrob Chemother. 2017;72(6):1669\u20131677. https:\/\/doi.org\/10.1093\/jac\/dkx021","DOI":"10.1093\/jac\/dkx021"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Nikolayevskyy V, Maurer FP, Holicka Y, Taylor L, Liddy H, Kranzer K. Novel external quality assurance scheme for drug susceptibility testing of non-tuberculous mycobacteria: A multicentre pilot study. J Antimicrob Chemother. 2019;74(5):1288\u20131294. https:\/\/doi.org\/10.1093\/jac\/dkz027","DOI":"10.1093\/jac\/dkz027"},{"key":"ref22","unstructured":"Hain-Lifescience. GenoType NTM-DR VER 1.0 Instructions for Use [homepage on the Internet]. 2015 [cited 2022 Mar 15]. Available from: http:\/\/www.hain-lifescience.de\/include_datei\/kundenmodule\/packungsbeilage\/download.php?id=981"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Kehrmann J, Kurt N, Rueger K, Bange FC, Buer J. GenoType NTM-DR for identifying Mycobacterium abscessus subspecies and determining molecular resistance. J Clin Microbiol. 2016;54(6):1653\u20131655. https:\/\/doi.org\/10.1128\/JCM.00147-16","DOI":"10.1128\/JCM.00147-16"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Huh HJ, Kim SY, Shim HJ, et al. GenoType NTM-DR performance evaluation for identification of Mycobacterium avium complex and Mycobacterium abscessus and determination of clarithromycin and amikacin resistance. J Clin Microbiol. 2019;57(8):e00516-19. https:\/\/doi.org\/10.1128\/JCM.00516-19","DOI":"10.1128\/JCM.00516-19"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Mbeha B, Mine M, Motswaledi MS, Dewar J. Nontuberculous mycobacteria, Botswana, 2011\u20132014. Emerg Infect Dis. 2019;25(7):1401\u20131403. https:\/\/doi.org\/10.3201\/eid2507.181440","DOI":"10.3201\/eid2507.181440"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Sookan L, Coovadia YM. A laboratory-based study to identify and speciate nontuberculous mycobacteria isolated from specimens submitted to a central tuberculosis laboratory from throughout KwaZulu-Natal Province, South Africa. 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