{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:39:24Z","timestamp":1786653564171,"version":"build-2736575974"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013]]},"abstract":"\n Background and Objective:<\/jats:title>\n \n Multidrug resistant tuberculosis (MDR-TB) is caused by infection due to\n Mycobacterium tuberculosis<\/jats:italic>\n which is resistant to both isoniazid (INH) and rifampicin (RIF). It is caused by selection of resistant mutant strains due to inadequate treatment and poor compliance. MDR-TB is a major public health problem as the treatment is complicated, cure rates are well below those for drug susceptible tuberculosis and patient remains infectious for months despite receiving the best available therapy. The drug susceptibility pattern of\n M. tuberculosis<\/jats:italic>\n is essential for proper control of MDR-TB in every health care setting, hence the study was initiated with the aim of studying the prevalence of MDR-TB in patients attending a tertiary care hospital in east Delhi.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n Five hundred and forty-three pulmonary and extrapulmonary samples from suspected cases of tuberculosis received in the mycobacteriology laboratory from November 2009 through October 2010 were investigated for\n M. tuberculosis<\/jats:italic>\n . All the samples were subjected to direct microscopic examination for demonstration of acid fast bacilli followed by culture on Lowenstein-Jensen (LJ) medium to isolate\n M. tuberculosis<\/jats:italic>\n . Identification was done by conventional biochemical methods. Drug susceptibility of isolated\n M. tuberculosis<\/jats:italic>\n strains was done by conventional 1% proportion method followed by sequencing of RIF resistant isolates to detect mutations to confirm resistance.\n <\/jats:p>\n <\/jats:sec>\n \n Results and Conclusions:<\/jats:title>\n \n M. tuberculosis<\/jats:italic>\n was isolated from 75 out of 543 suspected cases of pulmonary\/extrapulmonary TB. Three of the total 75\n M. tuberculosis<\/jats:italic>\n isolates (4%) showed resistance to any one of the first line drugs. Prevalence of MDR-TB was 1.3%. The sequencing of single MDR strain showed mutations at codons 516, 517, and 518. Amplification of\n rpoB<\/jats:italic>\n and sequential analysis of the amplicon is a better way of detection of mutation and the evidence of new mutation in this study indicate that mutations continue to arise, probably due to the ability of\n M. tuberculosis<\/jats:italic>\n to adapt to drug exposure.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0022-3859.118031","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T04:17:55Z","timestamp":1378959475000},"page":"173-176","source":"Crossref","is-referenced-by-count":5,"title":["Current status of multidrug resistant tuberculosis in a tertiary care hospital of East Delhi"],"prefix":"10.4103","volume":"59","author":[{"given":"T","family":"Sagar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NP","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Kashyap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IR","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R5-2-20240205","doi-asserted-by":"crossref","first-page":"1095","DOI":"10.1128\/jcm.32.4.1095-1098.1994","article-title":"Characterization by Automated DNA Sequencing of Mutations in the Gene (rpoB) Encoding the RNA Polymerase 3 Subunit in Rifampin-Resistant Mycobacterium tuberculosis Strains from New York City and Texas","volume":"32","author":"Kapur","year":"1994","journal-title":"J Clin 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tuberculosis in Lucknow, Uttar Pradesh","volume":"128","author":"Jain","year":"2008","journal-title":"Indian J Med Res"},{"key":"R14-2-20240205","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1128\/JCM.37.8.2663-2666.1999","article-title":"Mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis strains isolated mostly in Asian countries and their rapid detection by line probe assay","volume":"37","author":"Hirano","year":"1999","journal-title":"J Clin Microbiol"},{"key":"R15-2-20240205","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1186\/1471-2334-5-62","article-title":"A commercial line probe assay for the rapid detection of rifampicin resistance in Mycobacterium tuberculosis: A systematic review and meta-analysis","volume":"5","author":"Morgan","year":"2005","journal-title":"BMC Infect Dis"},{"key":"R16-2-20240205","doi-asserted-by":"crossref","first-page":"2987","DOI":"10.1128\/JCM.39.8.2987-2990.2001","article-title":"Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates from India","volume":"39","author":"Mani","year":"2001","journal-title":"J Clin Microbiol"},{"key":"R17-2-20240205","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/JCM.36.1.20-23.1998","article-title":"Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Greece","volume":"36","author":"Matsiota-Bernard","year":"1998","journal-title":"J Clin Microbiol"},{"key":"R18-2-20240205","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1128\/AAC.38.4.805","article-title":"The rpoB gene of Mycobacterium tuberculosis","volume":"38","author":"Miller","year":"1994","journal-title":"Antimicrob Agents Chemother"},{"key":"R19-2-20240205","doi-asserted-by":"crossref","first-page":"1197","DOI":"10.1128\/JCM.37.4.1197-1199.1999","article-title":"rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy","volume":"37","author":"Pozzi","year":"1999","journal-title":"J Clin Microbiol"},{"key":"R20-2-20240205","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1128\/JCM.39.2.636-641.2001","article-title":"Analysis for limited number of gene codon can predict drug resistance of Mycobacterium tuberculosis in a high-incidence community","volume":"39","author":"Van Rie","year":"2001","journal-title":"J Clin Microbiol"},{"key":"R21-2-20240205","doi-asserted-by":"crossref","first-page":"3736","DOI":"10.1128\/JCM.39.10.3736-3739.2001","article-title":"Molecular characterization of rifampin resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay","volume":"39","author":"B\u00e1rtfai","year":"2001","journal-title":"J Clin Microbiol"},{"key":"R22-2-20240205","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1128\/CMR.8.4.496","article-title":"Antimicrobial agent resistance in mycobacteria: Molecular genetic insights","volume":"8","author":"Musser","year":"1995","journal-title":"Clin Microbiol Rev"}],"container-title":["Journal of Postgraduate Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/00005257-201359030-00002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T19:39:22Z","timestamp":1786649962000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/00005257-201359030-00002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013]]}},"URL":"https:\/\/doi.org\/10.4103\/0022-3859.118031","relation":{},"ISSN":["0022-3859"],"issn-type":[{"value":"0022-3859","type":"print"}],"subject":[],"published":{"date-parts":[[2013]]}}}