{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:39:23Z","timestamp":1786653563296,"version":"build-2736575974"},"reference-count":38,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013]]},"abstract":"\n Background:<\/jats:title>\n \n The emergence of extensively drug-resistant tuberculosis (XDR-TB) is a major concern in the India. The burden of XDR-TB is increasing due to inadequate monitoring, lack of proper diagnosis, and treatment. The GenoType\n \u00ae<\/jats:sup>\n Mycobacterium tuberculosis<\/jats:italic>\n drug resistance second line (MTBDR\n sl<\/jats:italic>\n ) assay is a novel line probe assay used for the rapid detection of mutational patterns conferring resistance to XDR-TB.\n <\/jats:p>\n <\/jats:sec>\n \n Aim:<\/jats:title>\n \n The aim of this study was to study the rapid detection of drug resistance and mutational patterns of the XDR-TB by a novel GenoType\n \u00ae<\/jats:sup>\n MTBDR\n sl<\/jats:italic>\n assay.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n \n We evaluated 98 multidrug-resistant (MDR)\n M. tuberculosis<\/jats:italic>\n isolates for second line drugs susceptibility testing by 1% proportion method (BacT\/ALERT 3D system) and GenoType\n \u00ae<\/jats:sup>\n MTBDR\n sl<\/jats:italic>\n assay for rapid detection of conferring drug resistance to XDR-TB.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n A total of seven (17.4%) were identified as XDR-TB by using standard phenotypic method. The concordance between phenotypic and GenoType\n \u00ae<\/jats:sup>\n MTBDR\n sl<\/jats:italic>\n assay was 91.7-100% for different antibiotics. The sensitivity and specificity of the MTBDR\n sl<\/jats:italic>\n assay were 100% and 100% for aminoglycosides; 100% and 100% for fluoroquinolones; 91.7% and 100% for ethambutol. The most frequent mutations and patterns were\n gyrA<\/jats:italic>\n MUT1 (A90V) in seven (41.2%) and\n gyrA<\/jats:italic>\n +\n WT1-3<\/jats:italic>\n + MUT1 in four (23.5%);\n rrs<\/jats:italic>\n MUT1 (A1401G) in 11 (64.7%), and\n rrs WT1-2<\/jats:italic>\n + MUT1 in eight (47.1%); and\n embB<\/jats:italic>\n MUT1B (M306V) in 11 (64.7%) strains.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions:<\/jats:title>\n \n These data suggest that the GenoType\n \u00ae<\/jats:sup>\n MTBDR\n sl<\/jats:italic>\n assay is rapid, novel test for detection of resistance to second line anti-tubercular drugs. This assay provides additional information about the frequency and mutational patterns responsible for XDR-TB resistance.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0022-3859.118034","type":"journal-article","created":{"date-parts":[[2013,9,12]],"date-time":"2013-09-12T04:17:55Z","timestamp":1378959475000},"page":"179-185","source":"Crossref","is-referenced-by-count":9,"title":["Rapid detection of drug resistance and mutational patterns of extensively drug-resistant strains by a novel GenoType\u00ae MTBDRsl 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