{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:20:18Z","timestamp":1787761218312,"version":"build-2784847793"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Ca\n                      <jats:sup>2+<\/jats:sup>\n                      -sensing receptors (CaSRs) are G protein-coupled receptors activated by elevated concentrations of extracellular Ca\n                      <jats:sup>2+<\/jats:sup>\n                      . In our previous works, we showed protein and functional expression of CaSR in mouse cerebral endothelial cell (EC) (bEND.3); the CaSR response (high Ca\n                      <jats:sup>2+<\/jats:sup>\n                      -elicited cytosolic [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ] elevation) was unaffected by suppression of phospholipase C but in part involved Ca\n                      <jats:sup>2+<\/jats:sup>\n                      influx through transient receptor potential V1 (TRPV1) channels. In this work, we investigated if extracellular acidity affected CaSR-mediated Ca\n                      <jats:sup>2+<\/jats:sup>\n                      influx triggered by high (3 mM) Ca\n                      <jats:sup>2+<\/jats:sup>\n                      (CaSR agonist), 3 mM spermine (CaSR agonist), and 10 mM cinacalcet (positive allosteric modulator of CaSR). Extracellular acidosis (pH 6.8 and pH 6.0) strongly suppressed cytosolic [Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ] elevation triggered by high Ca\n                      <jats:sup>2+<\/jats:sup>\n                      , spermine, and cinacalcet; acidosis also inhibited Mn\n                      <jats:sup>2+<\/jats:sup>\n                      influx stimulated by high Ca\n                      <jats:sup>2+<\/jats:sup>\n                      and cinacalcet. Purinoceptor-triggered Ca\n                      <jats:sup>2+<\/jats:sup>\n                      response, however, was not suppressed by acidosis. Extracellular acidity also did not affect membrane potential, suggesting suppressed CaSR-mediated Ca\n                      <jats:sup>2+<\/jats:sup>\n                      influx in acidity did not result from the reduced electrical driving force for Ca\n                      <jats:sup>2+<\/jats:sup>\n                      . Our results suggest Ca\n                      <jats:sup>2+<\/jats:sup>\n                      influx through a putative CaSR-TRP complex in bEND.3 EC was sensitive to extracellular pH.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0304-4920.365460","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T03:44:03Z","timestamp":1672026243000},"page":"277-281","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["Sensitivity of Ca2+-Sensing Receptor-Transient Receptor Potential-Mediated Ca2+ Influx to Extracellular Acidity in bEND.3 Endothelial 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