{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:42:30Z","timestamp":1787071350084,"version":"build-2736575974"},"reference-count":9,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,1]]},"abstract":"\n Objective:<\/jats:title>\n \n The objective of the following study is to detect genes encoding carbapenem resistance in\n Klebsiella pneumoniae<\/jats:italic>\n sepsis outbreak in a neonatal intensive care unit (NICU).\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods:<\/jats:title>\n Antibiotic sensitivity test was performed by standard Kirby Bauer disc diffusion technique and minimum inhibitory concentrations of antibiotics was determined by VITEK-2. Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of beta-lactamase encoding genes. Conjugation experiments were performed to determine the transferability of beta-lactamase. Isolate relatedness were determined by repetitive-element PCR (REP), enterobacterial repetitive intergenic consensus (ERIC) PCR and random amplified polymorphic deoxyribonucleic acid (RAPD).<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n \n All the isolates were completely resistant to the second and third generation cephalosporins tested as well as carbapenems. Susceptibility profiling of the isolates indicated that 100% retained susceptibility to tigecycline and colistin. Conjugation experiments indicated that\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n was<\/jats:italic>\n transferable and likely through a plasmid-mediated event. All the isolates showed the presence of\n bla<\/jats:italic>\n NDM-1<\/jats:sub>\n with co association of bla\n CTX-M-15<\/jats:sub>\n . REP-PCR, ERIC-PCR and RAPD revealed a single clonal type circulating in NICU environment.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n \n Co-production of NDM-1 with CTX-M-15 in\n K<\/jats:italic>\n .\n pneumoniae<\/jats:italic>\n isolates was detected for the first time in our NICU. Transmission of plasmid carrying these resistant genes to other members of\n Enterobacteriaceae<\/jats:italic>\n will increase the incidence of multidrug resistance. Early detection of these genes will help in prevention and adequate infection control by limiting the spread of these organisms.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0377-4929.130900","type":"journal-article","created":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T09:02:49Z","timestamp":1398675769000},"page":"65-68","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":17,"title":["Multidrug resistant NDM-1 metallo-beta-lactamase producing Klebsiella pneumoniae sepsis outbreak in a neonatal intensive care unit in a tertiary care center at central India"],"prefix":"10.4103","volume":"57","author":[{"given":"Atul","family":"Khajuria","sequence":"first","affiliation":[{"name":"Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok Kumar","family":"Praharaj","sequence":"additional","affiliation":[{"name":"Department of Microbiology, AIIMS, Bhubaneswar, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahadevan","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naveen","family":"Grover","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Armed Forces Medical College, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20250922","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/CMR.11.4.589","article-title":"Klebsiella spp. as nosocomial pathogens: Epidemiology, taxonomy, typing methods, and 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