{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:25:27Z","timestamp":1787772327781,"version":"build-2784847793"},"reference-count":21,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:sec>\n                    <jats:title>Aim:<\/jats:title>\n                    <jats:p>The aim of the present study was to evaluate the presence of Cytomegalovirus (CMV) and Epsteinbarr virus \u22121 (EBV-1)viruses in sub gingival plaque of chronic periodontitis (groupA), aggressive periodontitis patients (group B), periodontally healthy controls (group C) and to compare the clinical parameters between virus negative and positive sites in each of these groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Sixty subjects were included in the study and equally divided into the 3 groups (group A - 20, group B - 20, group C - 20). Sub gingival plaque samples were obtained from the 3 deepest periodontal pocket sites in case of subjects suffering from periodontitis, and from one random bleeding site per quadrant in healthy groups. Clinical parameters like plaque index (PI), gingival index (GI), pocket depth (PD) and clinical loss of attachment (CAL) were recorded. Viral Deoxyribonucleic acid (DNA) was extracted using Proteinase-K DNA Extraction method, and the presence of CMV and EBV-1 was detected by polymerase chain reaction and 2% agarose gel.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>Results of our study showed a 45% prevalence of CMV and EBV-1 in Aggressive periodontitis cases. Prevalence of CMV in chronic periodontitis and healthy subjects was 20% and 10%, respectively; while for EBV-1 it was 25% and 0%, respectively. In terms of comparison of the clinical parameters with virus presence, both CMV and EBV-1 positive sites showed a significantly higher mean pocket depth compared to virus negative sites.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>Our studyshowed that the prevalence of EBV1 was higher in chronic and aggressive periodontitis subjects compared to controls and the prevalence of CMV was higher in aggressive periodontitis patients. The virus positive sites showed higher pocket depth compared to virus negative sites.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0970-9290.104948","type":"journal-article","created":{"date-parts":[[2012,12,20]],"date-time":"2012-12-20T07:21:56Z","timestamp":1355988116000},"page":"454-458","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":29,"title":["Comparative analysis of presence of Cytomegalovirus (CMV) and Epsteinbarr virus \u22121 (EBV-1) in cases of chronic periodontitis and aggressive periodontitis with controls"],"prefix":"10.4103","volume":"23","author":[{"given":"Rupali","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ogoti","family":"Padmalatha","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gurumoorthy","family":"Kaarthikeyan","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N D","family":"Jayakumar","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheeja","family":"Varghese","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Saveetha Dental College and Hospitals, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khalefathullah","family":"Sherif","sequence":"additional","affiliation":[{"name":"Department of Virology, King Institute, Chennai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-20260302","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1600-0757.2005.00112.x","article-title":"Microbiology of periodontal diseases","volume":"38","author":"Haffajee","year":"2005","journal-title":"Periodontol 2000"},{"key":"R2-20260302","first-page":"353","article-title":"Short-term benefits of the adjunctive use of metronidazole plus amoxicillin in the microbial profile and in the clinical parameters of subjects with generalized aggressive periodontitis","volume":"37","author":"Mestnik","year":"2010","journal-title":"J ClinPeriodontol"},{"key":"R3-20260302","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1034\/j.1600-0765.2003.00659.x","article-title":"The herpes virus- Porphromonasgingivalis -periodontitis axis","volume":"38","author":"Slots","year":"2003","journal-title":"J Periodontal Res"},{"key":"R4-20260302","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1034\/j.1600-0765.2000.035001003.x","article-title":"Herpesviruses in human periodontal disease","volume":"35","author":"Contreras","year":"2000","journal-title":"Periodontol Res"},{"key":"R5-20260302","first-page":"723","article-title":"Effect of CMV on murine neutrophil migratory and phagocytic activities","volume":"38","author":"Bale","year":"1985","journal-title":"J Biol"},{"key":"R6-20260302","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1093\/clinids\/14.4.933","article-title":"Herpesviruses - Immune escape artists?","volume":"14","author":"Banks","year":"1992","journal-title":"Clin Infect Dis"},{"key":"R7-20260302","first-page":"176","article-title":"Patient with severe periodontitis and subgingival Epstein-Barr virus treated with antiviral therapy","volume":"42","author":"Sundea","year":"2008","journal-title":"J ClinVirol"},{"key":"R8-20260302","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1111\/j.1600-0765.2006.00865.x","article-title":"Epstein Barr in oral disease","volume":"41","author":"Slots","year":"2006","journal-title":"J Periodontal Res"},{"key":"R9-20260302","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/0092-8674(89)90394-2","article-title":"Viral Latency and transformation: The strategy of Epstein-Barr virus","volume":"58","author":"Klein","year":"1989","journal-title":"Cell"},{"key":"R10-20260302","first-page":"271","article-title":"Frequent double infection with Epstein-Barr virus and human herpesvirus-6 in patients with acute infectious mononucleosis","volume":"5","author":"Bertram","year":"1991","journal-title":"In vivo"},{"key":"R11-20260302","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/0003-9969(87)90153-1","article-title":"Effect of tar condensate from smoking tobacco ad.of mice with latent herpes simples virus","volume":"32","author":"Park","year":"1987","journal-title":"Arch Oral Biol"},{"key":"R12-20260302","first-page":"15","article-title":"Sampling of microorganisms associated with periodontal disease","volume":"1","author":"Tanner","year":"1986","journal-title":"Oral MicrobiolImmunol"},{"key":"R13-20260302","first-page":"289","article-title":"Detection of human virus in periodontal pockets using polymerase chain reaction","volume":"5","author":"Parra","year":"1996","journal-title":"Oral MicrobiolImmunol"},{"key":"R14-20260302","first-page":"420","article-title":"Herpes viral bacterial interaction in aggressive periodontitis","volume":"30","author":"Kamma","year":"2003","journal-title":"J ClinPeriodontol"},{"key":"R15-20260302","first-page":"3216","article-title":"Infection frequency of EBV in subgingival samples from patients with different periodontal status and its correlation with severity of periodontal lesions","volume":"85","author":"WU","year":"2005","journal-title":"Zhonghua Yi XueZaZhi"},{"key":"R16-20260302","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1902\/jop.2007.070252","article-title":"Contreras A. Subgingival Human Cytomegalovirus Correlates With Increased Clinical Periodontal Parameters and Bacterial Coinfection in Periodontitis","volume":"78","author":"Botero","year":"2007","journal-title":"J Periodontol"},{"key":"R17-20260302","doi-asserted-by":"crossref","first-page":"1479","DOI":"10.1902\/jop.2004.75.11.1479","article-title":"Association between human herpes virus and the severity of periodontitis","volume":"75","author":"Ling","year":"2004","journal-title":"J Periodontol"},{"key":"R18-20260302","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1902\/jop.2002.73.12.1437","article-title":"Detection of human herpes virus HCMV, EBV, HSV in patients with chronic periodontitis and relationship between virus and clinical parameters","volume":"73","author":"Saygun","year":"2002","journal-title":"J Periodontol"},{"key":"R19-20260302","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.1902\/jop.2003.74.11.1634","article-title":"Prevalence of human herpesviruses in patients with aggressive periodontitis","volume":"74","author":"Yapar","year":"2003","journal-title":"J Periodontol"},{"key":"R20-20260302","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1600-0765.2005.00770.x","article-title":"Real time PCR quantification of human cytomegalovirus and EBV in periodontal pockets and adjacent gingival of periodontitis lesions","volume":"40","author":"Kubar","year":"2005","journal-title":"J Periodontal Res"},{"key":"R21-20260302","first-page":"217","article-title":"Update on human cytomegalovirus in destructive periodontal disease","volume":"19","author":"Slots","year":"2004","journal-title":"Oral MicrobiolImmunol"}],"container-title":["Indian Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/01363779-201223040-00004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:39:05Z","timestamp":1787769545000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/01363779-201223040-00004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,7]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2012]]}},"URL":"https:\/\/doi.org\/10.4103\/0970-9290.104948","relation":{},"ISSN":["0970-9290","1998-3603"],"issn-type":[{"value":"0970-9290","type":"print"},{"value":"1998-3603","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,7]]},"assertion":[{"value":"2010-07-01","name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-11-25","name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}}]}}