{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T12:30:50Z","timestamp":1773232250560,"version":"3.50.1"},"reference-count":29,"publisher":"Scientific Scholar","issue":"5","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec>\n            <jats:title>Background &amp; objectives:<\/jats:title>\n            <jats:p>Brucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for identification and biotyping of <jats:italic toggle=\"yes\">Brucella<\/jats:italic>. The present study describes the isolation and biochemical, molecular characterization of <jats:italic toggle=\"yes\">Brucella melitensis<\/jats:italic> from patients suspected for human brucellosis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>The blood samples were collected from febrile patients suspected to have brucellosis. A total of 18 isolates were obtained from 102 blood samples subjected to culture. The characterization of these 18 isolates was done by growth on <jats:italic toggle=\"yes\">Brucella<\/jats:italic> specific medium, biochemical reactions, CO<jats:sub>2<\/jats:sub> requirement, H<jats:sub>2<\/jats:sub>S production, agglutination with A and M mono-specific antiserum, dye sensitivity to basic fuchsin and thionin. Further, molecular characterization of the isolates was done by amplification of <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic> species specific IS<jats:italic toggle=\"yes\">711<\/jats:italic> repetitive DNA fragment and 16S (rRNA) sequence analysis. PCR-restriction fragment length polymorphism (RFLP) analysis of <jats:italic toggle=\"yes\">omp2<\/jats:italic> locus and IS<jats:italic toggle=\"yes\">711<\/jats:italic> gene was also done for molecular characterization.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>All 102 suspected samples were subjected to bacteria isolation and of these, 18 isolates could be recovered on blood culture. The biochemical, PCR and PCR-RFLP and 16s rRNA sequencing revealed that all isolates were of <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic> and matched exactly with reference strain <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic> 16M.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interpretation &amp; conclusions:<\/jats:title>\n            <jats:p>The present study showed an overall isolation rate of 17.64 per cent for <jats:italic toggle=\"yes\">B. melitensis<\/jats:italic>. There is a need to establish facilities for isolation and characterization of <jats:italic toggle=\"yes\">Brucella<\/jats:italic> species for effective clinical management of the disease among patients as well as surveillance and control of infection in domestic animals. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/0971-5916.187115","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T04:42:37Z","timestamp":1470285757000},"page":"652-658","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":30,"title":["Isolation &amp; characterization of Brucella melitensis isolated from patients suspected for human brucellosis in 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