{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T04:22:29Z","timestamp":1751516549024,"version":"3.41.0"},"reference-count":20,"publisher":"Scientific Scholar","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec>\n            <jats:title>Background &amp; objectives:<\/jats:title>\n            <jats:p>It is important to understand the role of cell surface receptors in susceptibility to infectious diseases. CD155 a member of the immunoglobulin super family, serves as the poliovirus receptor (PVR). Heterozygous (Ala67Thr) polymorphism in <jats:italic toggle=\"yes\">CD155<\/jats:italic> has been suggested as a risk factor for paralytic outcome of poliovirus infection. The present study pertains to the development of a screening test to detect the single nucleotide (SNP) polymorphism in the <jats:italic toggle=\"yes\">CD155<\/jats:italic> gene.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>New primers were designed for PCR, sequencing and SNP analysis of Exon2 of <jats:italic toggle=\"yes\">CD155<\/jats:italic> gene. DNAs extracted from either whole blood (<jats:italic toggle=\"yes\">n<\/jats:italic>=75) or cells from oral cavity (<jats:italic toggle=\"yes\">n<\/jats:italic>=75) were used for standardization and validation of the SNP assay. DNA sequencing was used as the gold standard method.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>A new SNP assay for detection of heterozygous Ala67Thr genotype was developed and validated by testing 150 DNA samples. Heterozygous <jats:italic toggle=\"yes\">CD155<\/jats:italic> was detected in 27.33 per cent (41\/150) of DNA samples tested by both SNP detection assay and sequencing.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Interpretation &amp; conclusions:<\/jats:title>\n            <jats:p>The SNP detection assay was successfully developed for identification of Ala67Thr polymorphism in human <jats:italic toggle=\"yes\">PVR\/CD155<\/jats:italic> gene. The SNP assay will be useful for large scale screening of DNA samples.<\/jats:p>\n          <\/jats:sec>","DOI":"10.4103\/0971-5916.193281","type":"journal-article","created":{"date-parts":[[2016,11,11]],"date-time":"2016-11-11T04:55:01Z","timestamp":1478840101000},"page":"38-45","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Assay for identification of heterozygous single-nucleotide polymorphism (Ala67Thr) in human poliovirus receptor gene"],"prefix":"10.25259","volume":"144","author":[{"given":"Shyam Sundar","family":"Nandi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deepa Kailash","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jagadish 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