{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:09:55Z","timestamp":1772896195600,"version":"3.50.1"},"reference-count":20,"publisher":"Scientific Scholar","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,7]]},"abstract":"\n Background & objectives:<\/jats:title>\n Dengue fever (DF) is associated with significant morbidity and mortality in the tropical and sub-tropical regions of the world. Since there are no effective antiviral drugs for treatment, clinicians often rely on the accurate diagnosis of dengue fever to begin supportive therapy at early stages of the illness. The objective of this study was to develop an in-house dengue virus serotype 2 (DENV-2) non-structural protein- 5 (NS5) based indirect ELISA.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n DENV-2 was raised in Vero cells and the viral proteins were separated and subsequently the NS5 protein was eluted. Serum samples from primary and secondary dengue fever patients; and acute and convalescent samples from Japanese encephalitis (JE) and West Nile virus (WNV) cases were used to validate the ELISA.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n The assay was found to be 100 per cent specific in detecting DENV-2 specific antibodies from patient's serum. However, in terms of sensitivity, the assay could detect IgM antibodies only from 90 per cent of the primary dengue samples. The IgM\/IgG ratio of the primary and secondary samples was 7.24 and 0.64, respectively.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n The results indicate that the DENV-2 NS5 ELISA is dengue group specific and can be used to differentiate dengue infection from other circulating Flavivirus infections. This NS5 ELISA can also be used to distinguish between primary and secondary dengue fever on the basis of IgM\/IgG ratios. Further studies with larger sample sizes and different DENV serotypes are required to validate the ELISA.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0971-5916.193300","type":"journal-article","created":{"date-parts":[[2016,11,11]],"date-time":"2016-11-11T04:55:01Z","timestamp":1478840101000},"page":"128-133","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":18,"title":["A novel indirect ELISA for diagnosis of dengue fever"],"prefix":"10.25259","volume":"144","author":[{"given":"Rohan","family":"Narayan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Senthil","family":"Raja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Senthil","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohana","family":"Sambasivam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raja","family":"Jagadeesan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kavita","family":"Arunagiri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaveri","family":"Krishnasamy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunasekaran","family":"Palani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R1-20-20241212","first-page":"308","article-title":"Understanding dengue pathogenesis: implications for vaccine design","volume":"83","author":"Stephenson","year":"2005","journal-title":"Bull World Health Organ"},{"key":"R2-20-20241212","doi-asserted-by":"crossref","first-page":"e4945","DOI":"10.1371\/journal.pone.0004945","article-title":"Reliable classifier to differentiate primary and secondary acute dengue infection based on IgG ELISA","volume":"4","author":"Cordeiro","year":"2009","journal-title":"PLoS One"},{"key":"R3-20-20241212","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/infdis\/jiv082","article-title":"Robust and balanced immune responses to all 4 dengue virus serotypes following administration of a single dose of a live attenuated tetravalent dengue vaccine to healthy, flavivirus-naive adults","volume":"212","author":"Kirkpatrick","year":"2015","journal-title":"J Infect Dis"},{"key":"R4-20-20241212","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1177\/1535370212473703","article-title":"Challenges for the formulation of a universal vaccine against dengue","volume":"238","author":"Chokephaibulkit","year":"2013","journal-title":"Exp Biol Med"},{"key":"R5-20-20241212","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.1128\/JCM.00351-13","article-title":"Evaluation of a new commercially available immunoglobulin M capture enzyme-linked immunosorbent assay for diagnosis of dengue virus infection","volume":"51","author":"Namekara","year":"2013","journal-title":"J Clin Microbiol"},{"key":"R6-20-20241212","doi-asserted-by":"crossref","first-page":"1782","DOI":"10.1128\/JCM.42.4.1782-1784.2004","article-title":"Use of an immunoglobulin G avidity test to discriminate between primary and secondary dengue virus infections","volume":"42","author":"Ueda","year":"2004","journal-title":"J Clin Microbiol"},{"key":"R7-20-20241212","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1016\/j.jcv.2007.04.003","article-title":"Evaluation of immunoglobulin M and G capture enzyme-linked immunosorbent assay Panbio kits for diagnostic dengue infections","volume":"39","author":"Vazqueza","year":"2007","journal-title":"J Clin Virol"},{"key":"R8-20-20241212","first-page":"642","article-title":"Current advances in dengue diagnosis","volume":"11","author":"Shu","year":"2004","journal-title":"Clin Diagn Lab Immunol"},{"key":"R9-20-20241212","doi-asserted-by":"crossref","first-page":"622","DOI":"10.1128\/CDLI.10.4.622-630.2003","article-title":"Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections","volume":"10","author":"Shu","year":"2003","journal-title":"Clin Vaccine Immunol"},{"key":"R10-20-20241212","first-page":"5","article-title":"Laboratory diagnosis of dengue virus infection: current and future perspectives in clinical diagnosis and public health","volume":"38","author":"Kao","year":"2005","journal-title":"J Microbiol Immunol Infect"},{"key":"R11-20-20241212","first-page":"373","article-title":"Chaturvedi. 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