{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T23:40:08Z","timestamp":1751499608695,"version":"3.41.0"},"reference-count":29,"publisher":"Scientific Scholar","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2016,10]]},"abstract":"\n Background & objectives:<\/jats:title>\n In bladder outlet obstruction-induced rat models, the transforming growth factor-beta (TGF-\u03b2) and collagen ratios have been shown to be increased. Increased TGF-\u03b2 leads to fibrosis. In this study, the effect of omega-3 and interferon alpha-2b (IFN \u03b1-2b) was investigated on oxidative stress, inflammation and fibrosis in bladder structure in a partial bladder outlet obstruction (PBOO) rat model.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n A total of 35 male Wistar albino rats, weighing 300-350 g, were used in the study. The rats were randomly divided into five groups. At the end of the experimental period, bladders were harvested from all the rats, and pathological analysis of the rat bladder tissues was performed. In addition, investigations were carried out with enzymatic and non-enzymatic antioxidant systems to study the antioxidant properties of omega-3 fatty acid and IFN alpha-2b.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Increased bladder weight in the PBOO group, in comparison to the control group, was decreased by the administration of omega-3 and IFN \u03b1-2b (P<\/jats:italic>=0.002). Significantly higher superoxide dismutase (SOD) levels were detected in group 2 in comparison to the control group. It was also detected that serum SOD, glutathione peroxidase and nitric oxide (NO) levels were significantly higher in group 2 when compared to the control group (P<\/jats:italic><0.05). In the pathologic evaluation, group 2 showed significantly increased inflammation and fibrosis compared to the control group. Omega-3 treatment significantly decreased inflammation. It was shown that IFN \u03b1-2b application partially decreased inflammation.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n The results of the present study showed that in addition to the standard primary approaches to prevent the damage to the upper urinary tract secondary to PBOO, omega-3 fatty acid and IFN \u03b1-2b could be beneficial as adjunct treatment in clinical practice. However, this needs to be further investigated with prospective, randomized clinical trials with larger sample sizes.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0971-5916.200899","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T19:07:35Z","timestamp":1734635255000},"page":"572-579","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the effects of omega-3 & interferon alpha-2b administration on partial bladder outlet obstruction in a rat model"],"prefix":"10.25259","volume":"144","author":[{"given":"Fatih","family":"Firat","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nihat","family":"Uluocak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fikret","family":"Erdemir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dogan","family":"Atilgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatma","family":"Markoc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bekir Suha","family":"Parlaktas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adem","family":"Yasar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R1-13-20241212","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1111\/j.1464-410X.1989.tb05119.x","article-title":"Poor compliance early in filling in the neuropathic bladder","volume":"63","author":"Weston","year":"1989","journal-title":"Br J Urol"},{"key":"R2-13-20241212","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1097\/00005392-199909010-00071","article-title":"The hyaluronic acid receptor RHAMM is induced by stretch injury of rat bladder in vivo and influences smooth muscle cell contraction in vitro [corrected]","volume":"162","author":"B\u00e4gli","year":"1999","journal-title":"J Urol"},{"key":"R3-13-20241212","doi-asserted-by":"crossref","first-page":"2235","DOI":"10.1016\/S0022-5347(05)66173-3","article-title":"Extracellular matrix gene responses in a novel ex vivo model of bladder stretch injury","volume":"165","author":"Capolicchio","year":"2001","journal-title":"J Urol"},{"key":"R4-13-20241212","first-page":"R1960","article-title":"Dysregulated proteolytic balance as the basis of excess extracellular matrix in fibrotic disease","volume":"272","author":"Peters","year":"1997","journal-title":"Am J Physiol"},{"key":"R5-13-20241212","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.ejphar.2007.01.031","article-title":"Role of peripheral 5-HT1A receptors in detrusor over activity associated with partial bladder outlet obstruction in female rats","volume":"561","author":"Mittra","year":"2007","journal-title":"Eur J Pharmacol"},{"key":"R6-13-20241212","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.juro.2009.02.104","article-title":"Suppression of bladder oxidative stress and inflammation by a phytotherapeutic agent in a rat model of partial bladder outlet obstruction","volume":"182","author":"Oka","year":"2009","journal-title":"J Urol"},{"key":"R7-13-20241212","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1001\/jama.295.4.403","article-title":"Effects of omega-3 fatty acids on cancer risk: a systematic review","volume":"295","author":"Catherine","year":"2006","journal-title":"JAMA"},{"key":"R8-13-20241212","doi-asserted-by":"crossref","first-page":"532","DOI":"10.2174\/092986712798918851","article-title":"n-3 Fatty acid-derived lipid mediators in the brain: new weapons against oxidative stress and inflammation","volume":"19","author":"Farooqui","year":"2012","journal-title":"Curr Med Chem"},{"key":"R9-13-20241212","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ejogrb.2007.09.006","article-title":"Efficacy of recombinant human interferon alpha-2b on experimental endometriosis","volume":"139","author":"Altintas","year":"2008","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"R10-13-20241212","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1002\/ijc.2910420207","article-title":"Recombinant human leukocyte interferon-alpha 2b stimulates the synthesis and release of a 90K tumor-associated antigen in human breast cancer cells","volume":"42","author":"Iacobelli","year":"1988","journal-title":"Int J Cancer"},{"key":"R11-13-20241212","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1006\/gyno.1996.0068","article-title":"Interferon-alpha and all-trans-retinoic acid reversibly inhibit the in vitro proliferation of cell lines derived from cervical cancers","volume":"60","author":"Massad","year":"1996","journal-title":"Gynecol Oncol"},{"key":"R12-13-20241212","doi-asserted-by":"crossref","first-page":"1127","DOI":"10.1016\/j.urology.2005.06.070","article-title":"Evaluation of two techniques of partial urethral obstruction in the male rat model of bladder outlet obstruction","volume":"66","author":"Melman","year":"2005","journal-title":"Urology"},{"key":"R13-13-20241212","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0009-8981(93)90307-P","article-title":"A methodological approach to superoxide dismutase (SOD) activity assay based on inhibition of nitroblue tetrazolium (NBT) reduction","volume":"214","author":"Durak","year":"1993","journal-title":"Clin Chim Acta"},{"key":"R14-13-20241212","first-page":"158","article-title":"Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase","volume":"70","author":"Paglia","year":"1967","journal-title":"J Lab Clin Med"},{"key":"R15-13-20241212","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1016\/S0022-2275(20)40104-X","article-title":"A critical assessment of the effects of aminoguanidine and ascorbate on the oxidative modification of LDL: evidence for interference with some assays of lipoprotein oxidation by aminoguanidine","volume":"35","author":"Scaccini","year":"1994","journal-title":"J Lipid Res"},{"key":"R16-13-20241212","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/0076-6879(90)86141-H","article-title":"Determination of carbonyl content in oxidatively modified proteins","volume":"186","author":"Packer","year":"1990","journal-title":"Methods Enzymol Calif"},{"key":"R17-13-20241212","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/0076-6879(90)86134-H","article-title":"Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal","volume":"186","author":"Esterbauer","year":"1990","journal-title":"Methods Enzymol"},{"key":"R19-13-20241212","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1464-410X.2005.05589.x","article-title":"Effect of bladder ischaemia\/reperfusion on superoxide dismutase activity and contraction","volume":"96","author":"Erdem","year":"2005","journal-title":"BJU Int"},{"key":"R20-13-20241212","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1007\/s11010-005-9068-4","article-title":"Effect of bilateral in vivo ischemia\/reperfusion on the activities of superoxide dismutase and catalase: response to a standardized grape suspension","volume":"296","author":"Lin","year":"2007","journal-title":"Mol Cell Biochem"},{"key":"R21-13-20241212","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1016\/S0002-9440(10)63923-5","article-title":"Urinary outflow obstruction increases apoptosis and deregulates Bcl-2 and Bax expression in the fetal ovine bladder","volume":"162","author":"Thiruchelvam","year":"2003","journal-title":"Am J Pathol"},{"key":"R22-13-20241212","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1111\/j.1464-410X.2010.09445.x","article-title":"Bladder outlet obstruction: progression from inflammation to fibrosis","volume":"106","author":"Metcalfe","year":"2010","journal-title":"BJU Int"},{"key":"R23-13-20241212","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1002\/ptr.2909","article-title":"Eviprostat suppresses urinary oxidative stress in a rabbit model of partial bladder outlet obstruction and in patients with benign prostatic hyperplasia","volume":"24","author":"Matsumoto","year":"2010","journal-title":"Phytother Res"},{"key":"R24-13-20241212","doi-asserted-by":"crossref","first-page":"780","DOI":"10.1016\/j.juro.2009.09.074","article-title":"Bladder dysfunction in a new mutant mouse model with increased superoxide \u2013 Lack of nitric oxide?","volume":"183","author":"Soler","year":"2010","journal-title":"J Urol"},{"key":"R25-13-20241212","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1016\/0002-9610(91)91120-8","article-title":"Pharmacologic approach to tissue injury mediated by free radicals and other reactive oxygen metabolites","volume":"161","author":"Reilly","year":"1991","journal-title":"Am J Surg"},{"key":"R26-13-20241212","doi-asserted-by":"crossref","first-page":"F357","DOI":"10.1152\/ajprenal.00261.2005","article-title":"L-NAME, a nitric oxide synthase inhibitor, diminishes oxidative damage in urinary bladder partial outlet obstruction","volume":"290","author":"Conners","year":"2006","journal-title":"Am J Physiol Renal Physiol"},{"key":"R27-13-20241212","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1111\/j.1464-410X.2008.08324.x","article-title":"The effect of sildenafil citrate on bladder outlet obstruction: a mouse model","volume":"104","author":"Beamon","year":"2009","journal-title":"BJU Int"},{"key":"R28-13-20241212","first-page":"179","article-title":"Changes in smooth muscle cellphenotype and contractile function following ischemia-reperfusion injury in the rat urinary bladder","volume":"54","author":"Matsumoto","year":"2008","journal-title":"Hinyokika Kiyo"},{"key":"R29-13-20241212","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s11255-010-9771-2","article-title":"Differential effects of coenzyme Q10 and a-lipoic acid on two models of in vitro oxidative damage to the rabbit urinary bladder","volume":"43","author":"Li","year":"2011","journal-title":"Int Urol Nephrol"},{"key":"R30-13-20241212","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1002\/nau.10072","article-title":"Morphometric analysis of the collagen changes induced by subcutaneous injection of interferon-gamma after bladder outlet obstruction in the rat","volume":"22","author":"Kim","year":"2003","journal-title":"Neurourol Urodyn"}],"container-title":["Indian Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.lww.com\/02223309-201644040-00013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T22:58:25Z","timestamp":1751497105000},"score":1,"resource":{"primary":{"URL":"https:\/\/ijmr.org.in\/evaluation-of-the-effects-of-omega-3-interferon-alpha-2b-administration-on-partial-bladder-outlet-obstruction-in-a-rat-model\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016]]}},"URL":"https:\/\/doi.org\/10.4103\/0971-5916.200899","relation":{},"ISSN":["0971-5916"],"issn-type":[{"type":"print","value":"0971-5916"}],"subject":[],"published":{"date-parts":[[2016,10]]}}}