{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T08:32:37Z","timestamp":1774945957282,"version":"3.50.1"},"reference-count":20,"publisher":"Scientific Scholar","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,1]]},"abstract":"\n Background & objectives:<\/jats:title>\n Chronic myelogenous leukaemia (CML) is the commonest leukaemia in Asia. There is a paucity data on cytogenetic and molecular analyses of Indian CML patients. This apparently reflects the low availability of cytogenetic and molecular techniques in our country. This study aimed to document various types of BCR-ABL<\/jats:italic> fusion transcripts in different phases of CML and to compare the Ph chromosome positivity\/negativity vis-a-vis BCR-ABL<\/jats:italic> fusion transcripts in adult CML patients.<\/jats:p>\n <\/jats:sec>\n \n Methods:<\/jats:title>\n Between June 2004 and February 2009, 208 patients were diagnosed as CML in chronic phase (CP), accelerated phase (AP) and blast crisis (BC), according to standard criteria. Cytogenetic and molecular genetic analyses were performed in all patients. Various types of BCR-ABL<\/jats:italic> hybrid transcripts were compared with phases of CML and cytogenetic abnormalities.<\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n Among 208 CML patients, b3a2 BCR-ABL<\/jats:italic> transcripts were most commonly detected (66.82%) followed by b2a2 (28.84%), b3a2 + b2a2 (3.36%), b3a2 + e19a2 (0.48%) and b2a2 + e19a2 (0.48%). b3a2 transcripts were more frequently detected than b2a2 transcripts, in the whole group of 208 as well as in 183 CML-CP patients (P<\/jats:italic><0.0001). Ph chromosome was positive in 135 of 139 patients with b3a2 transcripts and 56 of 60 patients with b2a2 transcripts, difference not being significant. Additional cytogenetic abnormalities detected in 3.8 per cent patients in CML-CP and 44 per cent patients in CML-AP\/BC, did not show predilection for any BCR-ABL<\/jats:italic> transcript type.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions:<\/jats:title>\n This study documents higher Ph positivity (96.15%) by cytogenetic analysis among CML patients, as confirmed by qualitative reverse transcriptase-polymerase chain reaction (RT-PCR) analysis in a large patient group from north India. Both the techniques contribute towards understanding the disease biology, and have important implications for diagnosis and management of CML patients.<\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0971-5916.93423","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T21:54:59Z","timestamp":1330984499000},"page":"42-48","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":19,"title":["Cytogenetic & molecular analyses in adult chronic myelogenous leukaemia patients in north India"],"prefix":"10.25259","volume":"135","author":[{"given":"Maninder Singh","family":"Anand","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelam","family":"Varma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhash","family":"Varma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamer Singh","family":"Rana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pankaj","family":"Malhotra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","reference":[{"key":"R5-10-20241212","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1002\/ajh.21096","article-title":"Molecular diagnosis and monitoring in the clinical management of patients with chronic myelogenous leukemia treated with tyrosine kinase inhibitors","volume":"83","author":"Ou","year":"2008","journal-title":"Am J Hematol"},{"key":"R6-10-20241212","doi-asserted-by":"crossref","first-page":"2375","DOI":"10.1182\/blood.V88.7.2375.bloodjournal8872375","article-title":"The diversity of BCR-ABL fusion proteins and their relationship to leukemia phenotype","volume":"88","author":"Melo","year":"1996","journal-title":"Blood"},{"key":"R7-10-20241212","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0165-4608(01)00515-5","article-title":"BCR-ABL rearrangement frequencies in chronic myeloid leukemia and acute lymphoblastic leukemia in Ecuador, South America","volume":"132","author":"Paz-y-Mi\u00f1o","year":"2002","journal-title":"Cancer Genet Cytogenet"},{"key":"R8-10-20241212","first-page":"553","article-title":"Analysis of Bcr-abl type transcript and its relationship with platelet count in Mexican patients with chronic myeloid leukemia","volume":"139","author":"Rosas-Cabral","year":"2003","journal-title":"Gac Med Mex"},{"key":"R9-10-20241212","first-page":"605","article-title":"Frequencies of the breakpoint cluster region types of the BCR\/ABL fusion gene in Mexican Mestizo patients with chronic myelogenous leukemia","volume":"56","author":"Ruiz-Arg\u00fcelles","year":"2004","journal-title":"Rev Invest Clin"},{"key":"R10-10-20241212","first-page":"803","article-title":"Differential molecular response of the transcripts B2A2 and B3A2 to imatinib mesylate in chronic myeloid leukemia","volume":"4","author":"de Lemos","year":"2005","journal-title":"Genet Mol Res"},{"key":"R11-10-20241212","first-page":"247","article-title":"Frequency of BCR-ABL fusion transcripts in Iranian patients with chronic myeloid leukemia","volume":"11","author":"Yaghmaie","year":"2008","journal-title":"Arch Iran Med"},{"key":"R13-10-20241212","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1002\/ajh.20682","article-title":"Molecular profiling of chronic myeloid leukemia in eastern India","volume":"81","author":"Mondal","year":"2006","journal-title":"Am J Hematol"},{"key":"R14-10-20241212","doi-asserted-by":"crossref","first-page":"1138","DOI":"10.4238\/vol7-4gmr485","article-title":"Analysis and comparison of clinicohematological parameters and molecular and cytogenetic response of two Bcr\/Abl fusion transcripts","volume":"7","author":"Polampalli","year":"2008","journal-title":"Genet Mol Res"},{"key":"R15-10-20241212","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/s00277-009-0822-7","article-title":"Response to Imatinib mesylate in chronic myeloid leukemia patients with variant BCR-ABL fusion transcripts","volume":"89","author":"Sharma","year":"2010","journal-title":"Ann Hematol"},{"key":"R16-10-20241212","first-page":"148","article-title":"Cytology and cytogenetics in chronic myelogenous leukaemias","volume":"68","author":"Das","year":"1978","journal-title":"Indian J Med Res"},{"key":"R17-10-20241212","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1016\/0165-4608(91)90129-I","article-title":"Chromosomal characteristics of chronic and blastic phase of chronic myeloid leukemia. 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