{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:20:01Z","timestamp":1787761201453,"version":"build-2784847793"},"reference-count":19,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,4]]},"abstract":"\n \n \n Anaplasma phagocytophilum<\/jats:italic>\n is a gram-negative obligate intracellular tick-borne rickettsia with veterinary and public health importance worldwide. This organism is an etiologic agent of tick-borne fever (TBF) in domesticated animals and human granulocytic anaplasmosis (HGA) as well. Hard ticks (Ixodida: Ixodidae) are incriminated as the main biologic vectors for\n Anaplasma<\/jats:italic>\n spp. Studies represent that\n Ixodes<\/jats:italic>\n spp. are the main vectors for\n A. phago-cytophilum<\/jats:italic>\n and few reports hinted that other tick species may play this role. So, the goal of the presented work was to investigate the\n A. phagocytophilum<\/jats:italic>\n in 2000 hard ticks in Khuzestan province of Iran by specific nested-PCR performing two consecutive amplifications of 16SrRNA gene fragment with highly variable nucleotide region. Each reaction included 10 salivary glands of distinct tick species. Specific nested-PCR on accumulated salivary glands detected specific bands in 15.5% of reactions (31 of 200) in electrophoresis only in\n Rhipicephalus sanguineous<\/jats:italic>\n and\n Hyalomma marginatum<\/jats:italic>\n ticks. We concluded that the broad distribution of\n A. phagocytophilum<\/jats:italic>\n infection is not only is due to the existence of\n Ixodes<\/jats:italic>\n spp. but other hard ticks may also play a role in this issue.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0972-9062.316273","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T01:32:31Z","timestamp":1671586351000},"page":"115-118","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":2,"title":["First molecular survey of Anaplasma phagocytophilum in hard ticks (Ixodidae) from Southwestern Iran"],"prefix":"10.4103","volume":"58","author":[{"given":"Hossein","family":"Hamidinejat","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad HosseinRazi","family":"Jallali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somayeh","family":"Bahrami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Bagheri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-3-20240830","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.jaut.2017.11.007","article-title":"Tick-borne diseases and autoimmunity: A comprehensive review","volume":"88","author":"Rodr\u00edguez","year":"2017","journal-title":"J Autoimmun"},{"key":"R2-3-20240830","doi-asserted-by":"crossref","first-page":"e0005021","DOI":"10.1371\/journal.pntd.0005021","article-title":"Tick-borne diseases in Turkey: A review based on one health perspective","volume":"10","author":"Inci","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"R3-3-20240830","doi-asserted-by":"crossref","first-page":"719","DOI":"10.2217\/fmb.12.45","article-title":"Anaplasma phagocytophilum: deceptively simple or simply deceptive?","volume":"7","author":"Severo","year":"2012","journal-title":"Future Microbiol"},{"key":"R4-3-20240830","doi-asserted-by":"crossref","first-page":"31","DOI":"10.3389\/fcimb.2013.00031","article-title":"Anaplasma phagocytophilum \u2013 a widespread multi-host pathogen with highly adaptive strategies","volume":"3","author":"Stuen","year":"2013","journal-title":"Front Cell Infect Microbiol"},{"key":"R5-3-20240830","doi-asserted-by":"crossref","first-page":"844","DOI":"10.1638\/2017-0165.1","article-title":"First molecular detection of Anaplasma phagocytophilum in dromedaries (Camelus dromedarius).","volume":"49","author":"Bahrami","year":"2018","journal-title":"J Zoo Wildl Med"},{"key":"R6-3-20240830","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.parint.2017.11.002","article-title":"Molecular analysis of tick-borne protozoan and rickettsial pathogens in small ruminants from two South African provinces","volume":"67","author":"6- Ringo","year":"2017","journal-title":"Parasitol Int"},{"key":"R7-3-20240830","first-page":"27","article-title":"Human granulocytic ehrlichiosis in Europe","volume":"37","author":"Strle","year":"2004","journal-title":"I J Med Microbiol"},{"key":"R8-3-20240830","doi-asserted-by":"crossref","first-page":"2301","DOI":"10.12681\/jhvms.25076","article-title":"Concurrent occurrence of Anaplasma phagocytophilum and A. marginale in bovine peripheral blood samples from southwest of Iran","volume":"71","author":"Bahrami","year":"2020","journal-title":"J Hell Vet Med Soc"},{"key":"R9-3-20240830","first-page":"297","article-title":"First molecular survey on Anaplasma phagocytophilum revealed high prevalence in rural dogs from Khuzestan province, Iran","volume":"14","author":"Hamidinejat","year":"2018","journal-title":"Iran J Parasitol"},{"key":"R10-3-20240830","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1099\/00207713-51-6-2145","article-title":"Reorganization of genera in the families Rickettsiaceae and Anaplasma taceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and \u201cHGE agent\u201d as subjective synonyms of Ehrlichia phagocytophila","volume":"51","author":"Dumler","year":"2001","journal-title":"Int J Syst Evol Microbiol"},{"key":"R12-3-20240830","first-page":"37","article-title":"Molecular detection of Anaplasma phagocytophilum in carrier cattle of Iran - first documented report","volume":"1","author":"Noaman","year":"2009","journal-title":"Iran J Microbiol"},{"key":"R13-3-20240830","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1016\/S2222-1808(16)61131-3","article-title":"High infection of Anaplasma and Ehrlichia spp. among tick species collected from different geographical locations of Iran","volume":"6","author":"Tajedin","year":"2016","journal-title":"Asian Pac J Trop Dis"},{"key":"R14-3-20240830","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1051\/acarologia\/20164137","article-title":"Anaplasma spp. identification in hard ticks of Iran: First report of Anaplasma bovis in Haemaphysalis inermis.","volume":"56","author":"Pazhoom","year":"2016","journal-title":"Acarologia"},{"key":"R15-3-20240830","doi-asserted-by":"crossref","first-page":"409","DOI":"10.17221\/7680-VETMED","article-title":"Epidemiology of Theileria equi in Persian Arab horses from Iran","volume":"59","author":"Bahrami","year":"2014","journal-title":"Vet Med"},{"key":"R16-3-20240830","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1007\/s11250-016-1071-0","article-title":"Evaluation of oxidant\/antioxidant status, trace mineral levels, and erythrocyte osmotic fragility in goats naturally infected with Anaplasma ovis.","volume":"48","author":"Jalali","year":"2016","journal-title":"Trop Anim Health Prod"},{"key":"R17-3-20240830","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.actatropica.2016.12.010","article-title":"Molecular epidemiological survey of bacterial and parasitic pathogens in hard ticks from eastern China","volume":"167","author":"Liu","year":"2017","journal-title":"Acta Trop"},{"key":"R18-3-20240830","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.jare.2011.08.002","article-title":"Prevalence and first molecular characterization of Anaplasma phagocytophilum, the agent of human granulocytic anaplasmosis, in Rhipicephalus sanguineusticks attached to dogs from Egypt","volume":"3","author":"Ghafar","year":"2012","journal-title":"J Adv Res"},{"key":"R19-3-20240830","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1111\/j.1469-0691.2008.02169.x","article-title":"The prevalence of Anaplasma phagocytophilum in host-seeking Ixodes ricinus ticks in Norway","volume":"15","author":"Rosef","year":"2009","journal-title":"Clin Microbiol Infect"},{"key":"R20-3-20240830","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.ttbdis.2012.11.006","article-title":"Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in Ixodes ricinus ticks in Br\u00f8nn\u00f8ysund in northern Norway","volume":"4","author":"Soleng","year":"2013","journal-title":"Ticks Tick Borne Dis"}],"container-title":["Journal of Vector Borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.316273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:26:32Z","timestamp":1787757992000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.316273"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.4103\/0972-9062.316273","relation":{},"ISSN":["0972-9062"],"issn-type":[{"value":"0972-9062","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4]]}}}