{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:44:05Z","timestamp":1791333845099,"version":"4.3.1"},"reference-count":22,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Background &amp; objectives:<\/jats:italic>\n                      There is limited evidence regarding the accuracy of dengue rapid diagnostic kits despite their extensive use in India. We evaluated the performance of four immunochromatographic Rapid Diagnostic Test (RDTs) kits: Multisure dengue Ab\/Ag rapid test (MP biomedicals; MP), Dengucheck combo (Zephyr Biomedicals; ZB), SD bioline dengue duo (Alere; SD) and Dengue day 1 test (J Mitra; JM).\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Methods:<\/jats:italic>\n                      This is a laboratory-based diagnostic evaluation study. Rapid tests results were compared to reference non-structural (NS1) antigen or immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) results of 241 dengue-positive samples and 247 dengue-negative samples. Sensitivity and specificity of NS1 and IgM components of each RDT were calculated separately and in combination (either NS1 or IgM positive) against reference standard ELISA.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                      A total of 238, 226, 208, and 146 reference NS1 ELISA samples were tested with MP, ZB, SD, and JM tests, respectively. In comparison to the NS1 ELISA reference tests, the NS1 component of MP, ZB, SD, and JM RDTs demonstrated a sensitivity of 71.8%, 85.1%, 77.2% and 80.9% respectively and specificity of 90.1%, 92.8%, 96.1 %, and 93.6%, respectively. In comparison to the IgM ELISA reference test, the IgM component of RDTs showed a sensitivity of 40.0%, 50.3%, 47.3% and 20.0% respectively and specificity of 92.4%, 88.6%, 96.5%, and 92.2% respectively. Combining NS1 antigen and IgM antibody results led to sensitivities of 87.5%, 82.9%, 93.8% and 91.7% respectively, and specificities of 75.3%, 73.9%, 76.5%, and 80.0% respectively.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Interpretation &amp; conclusion:<\/jats:italic>\n                      Though specificities were acceptable, the sensitivities of each test were markedly lower than manufacturers\u2019 claims. These results also support the added value of combined antigen-and antibody-based RDTs for the diagnosis of acute dengue.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0972-9062.321747","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T01:32:31Z","timestamp":1671586351000},"page":"159-164","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":13,"title":["Diagnostic accuracy of commercially available immunochromatographic rapid tests for diagnosis of dengue in India"],"prefix":"10.4103","volume":"58","author":[{"given":"Raman","family":"Mahajan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohit","family":"Nair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana-Maria","family":"Saldanha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Harshana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan De Lima","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandita","family":"Basu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama Prasad","family":"Goswami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nemai","family":"Bhattacharya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhaswati","family":"Bandyopadhay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manideepa","family":"SenGupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moutusi","family":"Day","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"Flevaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marleen","family":"Boelaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakib","family":"Burza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-10-20240830","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1038\/nature12060","article-title":"The global distribution and burden of dengue","volume":"496","author":"Bhatt","year":"2013","journal-title":"Nature"},{"key":"R3-10-20240830","doi-asserted-by":"crossref","first-page":"e0006618","DOI":"10.1371\/journal.pntd.0006618","article-title":"Dengue infection in India: A systematic review and meta-analysis","volume":"12","author":"Ganeshkumar","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"R5-10-20240830","doi-asserted-by":"crossref","first-page":"62","DOI":"10.4103\/0972-5229.126074","article-title":"Tropical fevers: Management guidelines","volume":"18","author":"Singhi","year":"2014","journal-title":"Indian J Crit Care Med"},{"key":"R6-10-20240830","doi-asserted-by":"crossref","first-page":"S30","DOI":"10.1038\/nrmicro2459","article-title":"Evaluation of diagnostic tests: Dengue","volume":"8","author":"Peeling","year":"2010","journal-title":"Nat Rev Microbiol"},{"key":"R8-10-20240830","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1128\/CDLI.11.4.642-650.2004","article-title":"Current Advances in Dengue Diagnosis","volume":"11","author":"P-Y","year":"2004","journal-title":"Clin Vaccine Immunol"},{"key":"R9-10-20240830","doi-asserted-by":"crossref","first-page":"436","DOI":"10.3201\/eid1503.080923","article-title":"Evaluation of commercially available antidengue virus immunoglobulin M tests","volume":"15","author":"Hunsperger","year":"2009","journal-title":"Emerg Infect Dis"},{"key":"R11-10-20240830","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.diagmicrobio.2016.09.020","article-title":"Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection","volume":"87","author":"Vivek","year":"2017","journal-title":"Diagn Microbiol Infect Dis"},{"key":"R12-10-20240830","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.1002\/jmv.24764","article-title":"Utility of dengue NS1 antigen rapid diagnostic test for use in difficult to reach areas and its comparison with dengue NS1 ELISA and qRT-PCR","volume":"89","author":"Shukla","year":"2017","journal-title":"J Med Virol"},{"key":"R13-10-20240830","doi-asserted-by":"crossref","first-page":"30","DOI":"10.4103\/0972-9062.179216","article-title":"Comparative evaluation of validity and cost-benefit analysis of rapid diagnostic test (RDT) kits in diagnosis of dengue infection using composite reference criteria: A cross-sectional study from south India","volume":"53","author":"Mitra","year":"2016","journal-title":"J Vector Borne Dis"},{"key":"R14-10-20240830","doi-asserted-by":"crossref","first-page":"e012799","DOI":"10.1136\/bmjopen-2016-012799","article-title":"STARD 2015 guidelines for reporting diagnostic accuracy studies: Explanation and elaboration","volume":"6","author":"Cohen","year":"2016","journal-title":"BMJ Open"},{"key":"R15-10-20240830","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0090037","article-title":"Diagnosing dengue at the point-of-care: Utility of a rapid combined diagnostic kit in Singapore","volume":"9","author":"Gan","year":"2014","journal-title":"PLoS One"},{"key":"R16-10-20240830","first-page":"166","article-title":"Evaluation of efficacy of various immunochromatographic rapid tests for dengue diagnosis","volume":"30","author":"Naz","year":"2014","journal-title":"Pakistan J Med Sci"},{"key":"R18-10-20240830","doi-asserted-by":"crossref","first-page":"2095","DOI":"10.1128\/CVI.05285-11","article-title":"Evaluation of six commercial point-of-care tests for diagnosis of acute dengue infections: The need for combining NS1 antigen and IgM\/IgG antibody detection to achieve acceptable levels of accuracy","volume":"18","author":"Blacksell","year":"2011","journal-title":"Clin Vaccine Immunol"},{"key":"R19-10-20240830","doi-asserted-by":"crossref","first-page":"690","DOI":"10.4269\/ajtmh.2010.10-0117","article-title":"Early diagnosis of dengue infection using a commercial dengue duo rapid test kit for the detection of NS1, IGM, and IGG","volume":"83","author":"Wang","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"R21-10-20240830","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.1128\/JCM.03042-14","article-title":"Multicountry prospective clinical evaluation of two enzyme-linked immunosorbent assays and two rapid diagnostic tests for diagnosing dengue fever","volume":"53","author":"Pal","year":"2015","journal-title":"J Clin Microbiol"},{"key":"R22-10-20240830","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1128\/JCM.00521-16","article-title":"Use of a rapid test for diagnosis of dengue during suspected dengue outbreaks in resource-limited regions","volume":"54","author":"Hunsperger","year":"2016","journal-title":"J Clin Microbiol"},{"key":"R23-10-20240830","doi-asserted-by":"crossref","first-page":"e113411","DOI":"10.1371\/journal.pone.0113411","article-title":"Evaluation of dengue ns1 antigen rapid tests and elisa kits using clinical samples","volume":"9","author":"Pal","year":"2014","journal-title":"PLoS One"},{"key":"R24-10-20240830","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1086\/501358","article-title":"The Comparative Accuracy of 8 Commercial Rapid Immunochromatographic Assays for the Diagnosis of Acute Dengue Virus Infection","volume":"42","author":"Blacksell","year":"2006","journal-title":"Clin Infect Dis"},{"key":"R25-10-20240830","doi-asserted-by":"crossref","first-page":"4687182","DOI":"10.1155\/2017\/4687182","article-title":"Performance Evaluation of Commercial Dengue Diagnostic Tests for Early Detection of Dengue in Clinical Samples","volume":"2017","author":"Mat Jusoh","year":"2017","journal-title":"J Trop Med"},{"key":"R26-10-20240830","doi-asserted-by":"crossref","first-page":"e1199","DOI":"10.1371\/journal.pntd.0001199","article-title":"The diagnostic sensitivity of Dengue Rapid test assays is significantly enhanced by using a combined Antigen and Antibody testing approach","volume":"5","author":"Fry","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"R27-10-20240830","doi-asserted-by":"crossref","first-page":"548","DOI":"10.4103\/ijmr.IJMR_916_18","article-title":"Geographical distribution of primary & secondary dengue cases in India-2017: A cross-sectional multicentric study","volume":"149","author":"Rao","year":"2019","journal-title":"Indian J Med Res"},{"key":"R28-10-20240830","first-page":"1","article-title":"Co-Circulation of All Dengue Serotypes among Patients Attending a Tertiary Care Hospital in Kolkata","volume":"2","author":"Dey","year":"2018","journal-title":"Virol Immunol J"}],"container-title":["Journal of Vector Borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.321747","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:25:26Z","timestamp":1787757926000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.321747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.4103\/0972-9062.321747","relation":{},"ISSN":["0972-9062"],"issn-type":[{"value":"0972-9062","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4]]}}}