{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T23:56:39Z","timestamp":1788825399418,"version":"build-2803163510"},"reference-count":16,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,10]]},"abstract":"\n \n \n Background & objectives:<\/jats:italic>\n In India, vector-borne diseases, dengue and chikungunya are major public health concerns. In recent decades, dengue outbreaks have been reported in almost every part of India. In 2016, India recorded 101388 dengue cases and 210 deaths, including 4337 cases and six deaths in Delhi, whereas Chikungunya outbreaks were reported from several states in 2006, with 1.3 million cases. The Dengue virus (DENV) and Chikungunya virus (CHIKV) are both transmitted by the same\n Aedes<\/jats:italic>\n mosquito species. DENV and CHIKV co-infections have been reported in 13 of 98 countries, with both viruses being transmitted locally. The reasons for the sudden upsurge in cases of these diseases are undetermined.\n <\/jats:p>\n \n Methods:<\/jats:italic>\n From March to December 2016, a study was carried out in 66 localities of Delhi in collaboration with the Municipal Corporation of Delhi. Localities were selected on the basis of confirmed dengue cases reported during the last five years and the study area was visited once a month. A door-to-door entomological survey was conducted to identify\n Aedes<\/jats:italic>\n breeding in all water-filled containers in and around houses. Both immature and mature stages of\n Aedes<\/jats:italic>\n mosquitoes were collected. Mosquitoes were pooled (n\u226410 each for male and female) breeding site-wise and stored in Trizol at -80\u00b0C. The Chikungunya and dengue viruses were detected using a multiplex RT-PCR.\n Results:<\/jats:italic>\n A total of 981\n Aedes<\/jats:italic>\n mosquitos were distributed among 146 Pools, and DENV and CHIKV were detected using Multiplex Reverse Transcriptase-PCR. Chikungunya virus was identified in 19 pools of females captured adults, whereas dengue virus was found in 8 pools of females captured adults. There was no evidence of coinfection in any of the pools.\n <\/jats:p>\n \n Interpretation & conclusion:<\/jats:italic>\n In endemic areas, continuous surveillance for both dengue and Chikungunya viruses is required to identify and characterize these viral pathogens. This information will also help implement effective strategies to combat outbreaks produced by these emerging viral pathogens.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0972-9062.325638","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T15:21:34Z","timestamp":1716909694000},"page":"386-390","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Co-distribution of dengue and Chikungunya viruses in Aedes mosquitoes of Delhi, India"],"prefix":"10.4103","volume":"58","author":[{"given":"Kumar","family":"Vikram","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. N.","family":"Nagpal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev Kumar","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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