{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:19:22Z","timestamp":1787761162580,"version":"build-2784847793"},"reference-count":15,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Dengue, a vector-borne disease remains as one of the most serious public health problems globally. Incidence of this disease is on an increasing trend and currently over a billion people in tropical and subtropical regions are at risk. In the absence of an operational vaccine, prevention of dengue virus (DENV) is primarily focused upon controlling mosquito vectors. Mosquito vector surveillance programmes require simple and rapid tools to detect mosquitoes infected with DENV. Here, we tested the commercially available DENV\n                      <jats:italic toggle=\"yes\">Detect\u2122<\/jats:italic>\n                      NS1 ELISA kit (InBios International, Inc.) for detection of recombinant DENV-NS1 protein in\n                      <jats:italic toggle=\"yes\">Aedes<\/jats:italic>\n                      mosquito samples. The kit was evaluated to find out the minimum detection limit of recombinant DENV-2 NS1 protein following the manufacturer\u2019s instructions. Initially, the NS1 protein detection threshold of the kit was determined and later the assay was standardized for detection of NS1 protein in\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      mosquito pools containing 5, 10 and 25 mosquitoes. The ELISA kit displayed high sensitivity towards detection of recombinant dengue virus-2 NS1 protein in mosquito pools (up to 25 mosquitoes per pool) at 25 pico gram concentration. Since the commercial NS1 ELISA is highly sensitive and follows a very simple procedure, it could be employed for DENV surveillance in\n                      <jats:italic toggle=\"yes\">Aedes aegypti<\/jats:italic>\n                      mosquitoes, after carrying out laboratory and field bioassays with DENV infected specimens.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0972-9062.328975","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T01:11:26Z","timestamp":1671585086000},"page":"98-101","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Detection of recombinant dengue virus 2 NS1 protein in Aedes aegypti mosquitoes using commercial Dengue NS1 ELISA kit"],"prefix":"10.4103","volume":"59","author":[{"given":"Philip Raj","family":"Abraham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Sankari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep N","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwani","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-14-20240830","doi-asserted-by":"crossref","first-page":"712","DOI":"10.1016\/S1473-3099(16)00026-8","article-title":"The global burden of dengue: an analysis from the Global Burden of Disease Study 2013","volume":"16","author":"Stanaway","year":"2016","journal-title":"Lancet Infect Dis"},{"key":"R3-14-20240830","first-page":"272","article-title":"ELISA as an alternative tool for epidemiological surveillance for dengue in mosquitoes: a report from Thailand","volume":"44","author":"Srisuphanunt","year":"2007","journal-title":"J Vector Borne Dis"},{"key":"R4-14-20240830","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/0042-6822(88)90677-0","article-title":"Nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 2 virus, Jamaica genotype: comparative analysis of the full-length genome","volume":"165","author":"Deubel","year":"1988","journal-title":"Virology"},{"key":"R5-14-20240830","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/0042-6822(87)90458-2","article-title":"The nucleotide sequence of dengue type 4 virus: analysis of genes coding for nonstructural proteins","volume":"159","author":"Mackow","year":"1987","journal-title":"Virology"},{"key":"R6-14-20240830","doi-asserted-by":"crossref","first-page":"1165","DOI":"10.1086\/343813","article-title":"High circulating levels of the dengue virus non-structural protein NS1 early in dengue illness correlate with the development of dengue hemorrhagic fever","volume":"186","author":"Libraty","year":"2002","journal-title":"J Infect Dis"},{"key":"R7-14-20240830","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1128\/CVI.00229-06","article-title":"Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum","volume":"13","author":"Dussart","year":"2006","journal-title":"Clin Vaccine Immunol"},{"key":"R8-14-20240830","doi-asserted-by":"crossref","first-page":"696","DOI":"10.4269\/ajtmh.2010.10-0138","article-title":"Relationship between nonstructural protein 1 detection and plasma virus load in Dengue patients","volume":"83","author":"Thomas","year":"2010","journal-title":"Am J Trop Med Hyg"},{"key":"R9-14-20240830","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1128\/JCM.40.02.376-381.2002","article-title":"Enzyme-linked immunosorbent assay specific to Dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections","volume":"40","author":"Alcon","year":"2002","journal-title":"J Clin Microbiol"},{"key":"R10-14-20240830","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1002\/jmv.21814","article-title":"Clinical evaluation of the NS1 antigen-capture ELISA for early diagnosis of dengue virus infection in Brazil","volume":"82","author":"Castro-Jorge","year":"2010","journal-title":"J Med Virol"},{"key":"R11-14-20240830","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1089\/vbz.2010.0028","article-title":"Evaluation of the Dengue NS1 Ag Strip\u00ae for detection of dengue virus antigen in Aedes aegypti (Diptera: Culicidae)","volume":"11","author":"Tan","year":"2011","journal-title":"Vector Borne Zoonotic Dis"},{"key":"R12-14-20240830","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.jviromet.2012.03.033","article-title":"A portable approach for the surveillance of dengue virus-infected mosquitoes","volume":"183","author":"Muller","year":"2012","journal-title":"J Virol Methods"},{"key":"R13-14-20240830","doi-asserted-by":"crossref","first-page":"260","DOI":"10.4269\/ajtmh.2012.12-0477","article-title":"Detection of dengue virus NS1 antigen in infected Aedes aegypti using a commercially available kit","volume":"88","author":"Voge","year":"2013","journal-title":"Am J Trop Med Hyg"},{"key":"R14-14-20240830","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1111\/tmi.12555","article-title":"Surveillance of adult Aedes mosquitoes in Selangor, Malaysia","volume":"20","author":"Lau","year":"2015","journal-title":"Trop Med Int Health"},{"key":"R15-14-20240830","doi-asserted-by":"crossref","first-page":"566","DOI":"10.3343\/alm.2019.39.6.566","article-title":"Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM\/IgG Antibodies","volume":"39","author":"Lee","year":"2019","journal-title":"Ann Lab Med"},{"key":"R17-14-20240830","doi-asserted-by":"crossref","first-page":"e3193","DOI":"10.1371\/journal.pntd.0003193","article-title":"Evaluation of a dengue NS1 antigen detection assay sensitivity and specificity for the diagnosis of acute dengue virus infection","volume":"8","author":"Hermann","year":"2014","journal-title":"PLoS Negl Trop Dis"}],"container-title":["Journal of Vector Borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.328975","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:25:26Z","timestamp":1787757926000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.328975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/0972-9062.328975","relation":{},"ISSN":["0972-9062"],"issn-type":[{"value":"0972-9062","type":"print"}],"subject":[],"published":{"date-parts":[[2022,1]]}}}