{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T16:19:47Z","timestamp":1787761187506,"version":"build-2784847793"},"reference-count":8,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Background &amp; objectives:<\/jats:italic>\n                      Microscopy is considered as the gold standard for malaria diagnosis, however sub-microscopic infections can only be detected by Polymerase chain reaction, which demands high cost and elaborate laboratory setup. The Micro-chip PCR based Truenat Malaria Pv-Pf and Pf assay is a portable solution for detection of sub-microscopic\/asymptomatic cases of malaria in the field, three lots of which were evaluated for\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                      malaria.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Methods:<\/jats:italic>\n                      Three lots of Truenat\u00ae Malaria Pv-Pf and Pf assay (kits) were assessed using blood samples of\n                      <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      as well as malaria negative blood samples. DNA was extracted from the blood samples using the Trueprep Auto v2 Universal Cartridge based sample prep device and real time qPCR was performed using Truelab DUO micro PCR Analyzer with three lots of Truenat\u00ae Malaria Pv-Pf and Pf Assays. Mean, Standard deviation and one-way analysis of variance (ANOVA) was used to assess the significance of inter-lot variability in Cycle threshold values.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Results:<\/jats:italic>\n                      The Truenat\u00ae Malaria Pv-Pf and Pf assays identified the malaria parasites with 100% accuracy. Based on the test for variance (ANOVA) the inter-lot variability in cycle threshold values were not significant, indicating a high degree of precision.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Interpretation &amp; conclusion:<\/jats:italic>\n                      Based on high accuracy and precision between different lots, the Truenat\u00ae Malaria Pv-Pf and Pf assays were found to be suitable for the diagnosis of sub-microscopic infections in field conditions to provide support in elimination of malaria.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0972-9062.328978","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T01:11:26Z","timestamp":1671585086000},"page":"57-62","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":4,"title":["Validation of micro-chip based PCR assays for diagnosis of both Plasmodium falciparum and Plasmodium vivax"],"prefix":"10.4103","volume":"59","author":[{"given":"Syed Shah Areeb","family":"Hussian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijay P.","family":"Ojha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenakshi","family":"Jeena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manju","family":"Rahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupkumar","family":"Anvikar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh C.","family":"Dhiman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R2-8-20240830","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1603\/0022-2585(2005)042[0777:ACOPSA]2.0.CO;2","article-title":"Asymptomatic carriers of Plasmodium spp. as infection source for malaria vector mosquitoes in the Brazilian Amazon","volume":"42","author":"Alves","year":"2005","journal-title":"J Med Entomol"},{"key":"R3-8-20240830","doi-asserted-by":"crossref","first-page":"939","DOI":"10.4269\/ajtmh.2007.77.939","article-title":"High prevalence of asymptomatic Plasmodium falciparum infection in Gabonese adults","volume":"77","author":"Dal-Bianco","year":"2007","journal-title":"Am J Trop Med Hyg"},{"key":"R4-8-20240830","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1086\/599317","article-title":"High prevalence of asymptomatic Plasmodium falciparum Infections in a highland area of western Kenya: A cohort 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