{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T20:01:36Z","timestamp":1787169696375,"version":"build-2736575974"},"reference-count":14,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4]]},"abstract":"\n \n \n Background & objectives:<\/jats:italic>\n Coexistence of tick-borne diseases in some regions in Latin America makes the diagnosis difficult due to shared initial signs and symptoms. Rickettsiosis, Lyme disease and recently, scrub typhus are gaining more importance. The objective of this study is to develop a multiplex-PCR assay for a differential diagnosis of rickettsiosis, Lyme disease and scrub typhus.\n <\/jats:p>\n \n Methods:<\/jats:italic>\n By using bibliographic and bioinformatic analysis, we identify candidate regions to perform the multiplex- PCR assay for\n Rickettsia<\/jats:italic>\n sp.,\n Borrelia burgdorferi<\/jats:italic>\n and\n Orientia tsutsugamushi<\/jats:italic>\n as well as identify optimal melting temperature and sensibility analysis.\n <\/jats:p>\n \n Results:<\/jats:italic>\n We identified specific primer pairs for\n Rickettsia<\/jats:italic>\n sp,\n Borrelia burgdorferi<\/jats:italic>\n and\n Orientia tsutsugamushi<\/jats:italic>\n with different PCR fragment length but a common melting temperature, 58\u00b0C.\n <\/jats:p>\n \n Interpretation & conclusion:<\/jats:italic>\n We successfully developed a Multiplex PCR assay for differential diagnosis of rickettsiosis, Lyme disease and scrub typhus that could be a rapid and easy option in clinical and epidemiological practice.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.4103\/0972-9062.337506","type":"journal-article","created":{"date-parts":[[2022,2,9]],"date-time":"2022-02-09T20:16:56Z","timestamp":1644437816000},"page":"178-181","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":1,"title":["A Multiplex PCR assay for a differential diagnostic of rickettsiosis, Lyme disease and scrub typhus"],"prefix":"10.4103","volume":"59","author":[{"given":"Gaspar","family":"Peniche-Lara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isamara","family":"Moo-Salazar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla","family":"Dzul-Rosado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-9-20240830","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.3934\/mbe.2020225","article-title":"Global dynamics of tick-borne diseases","volume":"17","author":"Kashkynbayev","year":"2020","journal-title":"Math Biosci Eng"},{"key":"R3-9-20240830","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1097\/01.ASW.0000694148.60567.cf","article-title":"Exploring Tropical Infections: A Focus on Scrub Typhus","volume":"33","author":"Gill","year":"2020","journal-title":"Adv Skin Wound Care"},{"key":"R4-9-20240830","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1186\/s12916-020-01746-z","article-title":"When fever is not malaria in Latin America: a systematic review","volume":"18","author":"Moreira","year":"2020","journal-title":"BMC Med"},{"key":"R5-9-20240830","first-page":"158","article-title":"Borrelia Infection in Latin America","volume":"70","author":"Robles","year":"2018","journal-title":"Rev Invest Clin"},{"key":"R6-9-20240830","doi-asserted-by":"crossref","first-page":"569","DOI":"10.4067\/S0716-10182014000500009","article-title":"Clinical approach and main tick-borne rickettsiosis present in Latin America","volume":"31","author":"Abarca","year":"2014","journal-title":"Rev Chilena Infectol"},{"key":"R7-9-20240830","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1097\/MOH.0000000000000606","article-title":"Human babesiosis: recent advances and future challenges","volume":"27","author":"Lobo","year":"2020","journal-title":"Curr Opin Hematol"},{"key":"R8-9-20240830","doi-asserted-by":"crossref","first-page":"e0226781","DOI":"10.1371\/journal.pone.0226781","article-title":"Zoonotic Babesia: A scoping review of the global evidence","volume":"14","author":"Young","year":"2019","journal-title":"PLoS One"},{"key":"R9-9-20240830","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1590\/S0124-00642008000100016","article-title":"A high prevalence of antibodies against Bartonella and Babesia microti has been found in villages and urban populations in Cordoba, Colombia","volume":"10","author":"Buelvas","year":"2008","journal-title":"Rev Salud Publica (Bogota"},{"key":"R10-9-20240830","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.3201\/eid2411.170512","article-title":"Human Babesiosis, Yucatan State, Mexico, 2015","volume":"24","author":"Peniche-Lara","year":"2018","journal-title":"Emerg Infect Dis"},{"key":"R11-9-20240830","doi-asserted-by":"crossref","first-page":"2148","DOI":"10.3201\/eid2609.200918","article-title":"Molecular Description of a Novel Orientia Species Causing Scrub Typhus in Chile","volume":"26","author":"Abarca","year":"2020","journal-title":"Emerg Infect Dis"},{"key":"R12-9-20240830","doi-asserted-by":"crossref","first-page":"101503","DOI":"10.1016\/j.ttbdis.2020.101503","article-title":"Human seroepidemiology of Rickettsia and Orientia species in Chile - 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