{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:23:50Z","timestamp":1787757830677,"version":"build-2784847793"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Scrub typhus is a seriously neglected life threatening disease in Asia and the islands of the Pacific and Indian oceans. The causative agent is\n                      <jats:italic toggle=\"yes\">Orientia tsutsugamushi<\/jats:italic>\n                      , an obligate intra-cytosolic bacterium transmitted to vertebrate animals such as human or rodent by larval trombiculid mites (chiggers). Here, we conducted an outbreak investigation in Madhya Pradesh state in central India after sudden upsurge in scrub typhus cases and mortality in humans. Sixty-five live traps were placed for capturing the rodents. The captured rodent species were identified and the samples were subjected to nested PCR targeting 56kDa TSA gene. Analysis revealed for the first-time predominant circulation of Karp genotype in this region. The vector species involved in transmission is\n                      <jats:italic toggle=\"yes\">Leptotrobidium deliense<\/jats:italic>\n                      . In conclusion, we reported the circulation of Karp strain in outbreak regions of Madhya Pradesh and suggest that public health monitoring and surveillance needs to be improved. Studies on antigenic diversity, association of strains with clinical spectrum and pathogenicity in the local setup will be essential for development of region-specific diagnostics and vaccines.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0972-9062.345176","type":"journal-article","created":{"date-parts":[[2022,5,12]],"date-time":"2022-05-12T08:21:15Z","timestamp":1652343675000},"page":"182-185","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":8,"title":["Molecular epidemiology of Orientia tsutsugamushi from outbreak regions, Madhya Pradesh, central India"],"prefix":"10.4103","volume":"59","author":[{"given":"HV","family":"Manjunathachar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Tiwari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CG","family":"Raut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailendra K","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparup","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"R1-10-20240830","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1056\/NEJMp1608499","article-title":"Scrub typhus\u2014scientific neglect, ever-widening impact","volume":"375","author":"Walker","year":"2016","journal-title":"N Engl"},{"key":"R2-10-20240830","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1086\/596576","article-title":"Scrub typhus: the geographic distribution of phenotypic and genotypic variants of Orientiatsutsugamushi","volume":"48","author":"Kelly","year":"2009","journal-title":"Clin Infect Dis"},{"key":"R3-10-20240830","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.meegid.2015.09.008","article-title":"Genotype diversity and distribution of Orientiatsutsugamushi in scrub typhus patients and rodents in Shandong, northern China. Infection","volume":"36","author":"Zheng","year":"2015","journal-title":"Infect Genet Evol"},{"key":"R4-10-20240830","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3201\/eid2101.140580","article-title":"Molecular epidemiology and genetic diversity of Orientiatsutsugamushi from patients with scrub typhus in 3 regions of India","volume":"21","author":"Varghese","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"R5-10-20240830","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1186\/s13071-015-0784-7","article-title":"Detection of Orientia sp. DNA in rodents from Asia, West Africa and Europe","volume":"8","author":"Cosson","year":"2015","journal-title":"Parasites Vectors"},{"key":"R6-10-20240830","doi-asserted-by":"crossref","first-page":"954","DOI":"10.1056\/NEJMoa1603657","article-title":"Endemic scrub typhus in South America","volume":"375","author":"Weitzel","year":"2016","journal-title":"N Engl"},{"key":"R7-10-20240830","doi-asserted-by":"crossref","first-page":"e0006062","DOI":"10.1371\/journal.pntd.0006062","article-title":"A review of the global epidemiology of scrub typhus","volume":"11","author":"Xu","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"R8-10-20240830","doi-asserted-by":"crossref","first-page":"1590","DOI":"10.3201\/eid1210.051697","article-title":"Scrub typhus in Himalayas","volume":"12","author":"Mahajan","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"R9-10-20240830","doi-asserted-by":"crossref","first-page":"72","DOI":"10.4103\/0255-0857.108729","article-title":"Outbreak of scrub typhus in the North East Himalayan region-Sikkim: an emerging threat","volume":"31","author":"Gurung","year":"2013","journal-title":"Indian J Med Microbiol"},{"key":"R10-10-20240830","doi-asserted-by":"crossref","first-page":"893","DOI":"10.4103\/ijmr.IJMR_1390_15","article-title":"Abundance & distribution of trombiculid mites &Orientiatsutsugamushi, the vectors & pathogen of scrub typhus in rodents & shrews collected from Puducherry & Tamil Nadu, India","volume":"144","author":"Candasamy","year":"2016","journal-title":"IJMR"},{"key":"R11-10-20240830","first-page":"e889","article-title":"Re-emergence of scrub typhus in northeast India","volume":"16","author":"Khan","year":"2012","journal-title":"IJID"},{"key":"R12-10-20240830","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol Biol Evol"},{"key":"R13-10-20240830","doi-asserted-by":"crossref","first-page":"1936","DOI":"10.1016\/S0140-6736(12)61678-X","article-title":"Ecology of zoonoses: natural and unnatural histories","volume":"380","author":"Karesh","year":"2012","journal-title":"Lancet"},{"key":"R14-10-20240830","doi-asserted-by":"crossref","first-page":"7039","DOI":"10.1073\/pnas.1501598112","article-title":"Rodent reservoirs of future zoonotic diseases","volume":"112","author":"Han","year":"2015","journal-title":"PNAS"},{"key":"R15-10-20240830","doi-asserted-by":"crossref","first-page":"e22731","DOI":"10.1371\/journal.pone.0022731","article-title":"Differences in clinical features according to Boryoung and Karp genotypes of Orientiatsutsugamushi","volume":"6","author":"Kim","year":"2011","journal-title":"PloS one"},{"key":"R16-10-20240830","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1111\/j.1348-0421.1996.tb01135.x","article-title":"Virulence in mice of Orientiatsutsugamushi isolated from patients in a new endemic area in Japan","volume":"40","author":"Nagano","year":"1996","journal-title":"Microbiol Immunol"},{"key":"R17-10-20240830","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1089\/vbz.2017.2159","article-title":"Prevalence and phylogenetic analysis of Orientia tsutsugamushi in rodents and mites from central India","volume":"17","author":"Bhate","year":"2017","journal-title":"Vector Borne Zoonotic Dis"},{"key":"R18-10-20240830","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1089\/vbz.2017.2246","article-title":"Occurrence of Orientia tsutsugamushi, the etiological agent of scrub typhus in animal hosts and mite vectors in areas reporting human cases of acute encephalitis syndrome in the Gorakhpur region of Uttar Pradesh, India","volume":"18","author":"Sadanandane","year":"2018","journal-title":"Vector- Borne and Zoonotic Dis"}],"container-title":["Journal of Vector Borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.345176","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T14:54:06Z","timestamp":1787756046000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ovid.com\/10.4103\/0972-9062.345176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022]]}},"URL":"https:\/\/doi.org\/10.4103\/0972-9062.345176","relation":{},"ISSN":["0972-9062"],"issn-type":[{"value":"0972-9062","type":"print"}],"subject":[],"published":{"date-parts":[[2022,4]]}}}