{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T17:23:42Z","timestamp":1787765022242,"version":"build-2784847793"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:sec>\n                    <jats:title>Background &amp; objectives:<\/jats:title>\n                    <jats:p>\n                      Malaria is a parasitic disease spread by\n                      <jats:italic toggle=\"yes\">Plasmodium<\/jats:italic>\n                      parasite. Microscopy, lateral flow devices such as the Rapid Diagnostic Test (RDT), molecular methods such as Polymerase Chain Reaction (PCR), isothermal methods such as Loop-mediated isothermal amplification (LAMP), and other diagnostic methods are available for malaria. On the other hand, the accuracy of molecular diagnosis is dependent on genomic DNA isolation. A quick method for isolating and subjectively determining the presence of genomic DNA from blood, dried blood spot (DBS), and rapid diagnostic test (RDT), was identified.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>We have developed a protocol for isolating DNA from blood, DBS, and RDTs using the HUDSON Buffer (TCEP and EDTA). Isolated genomic DNA was seen with SYBR Safe DNA stain (1X) under a UV transilluminator without running in 0.8 percent gel electrophoresis or using a spectrophotometer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The technique for DNA isolation was accurate for the presence of malaria parasite genomic DNA from positive samples confirmed by microscopy with a sensitivity of 76% and specificity of 78.67% and RDT with a sensitivity of 88% and specificity of 66%. The requirements were minimal, and the process took 30 minutes for a hundred sample processing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation &amp; conclusion:<\/jats:title>\n                    <jats:p>Finding a fast and reliable method of separating nucleic acids from many samples is crucial. This approach extracts intact genomic DNA in under ten minutes, making it ideal for large-scale investigations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.4103\/0972-9062.383646","type":"journal-article","created":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T05:01:06Z","timestamp":1703566866000},"page":"449-452","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":0,"title":["A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test"],"prefix":"10.4103","volume":"60","author":[{"given":"Supriya","family":"Sharma","sequence":"first","affiliation":[{"name":"ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparajita","family":"Sen","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naseem","family":"Ahmed","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deendayal","family":"Swarnkar","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Himmat","family":"Singh","sequence":"additional","affiliation":[{"name":"ICMR-National Institute of Malaria Research, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","published-online":{"date-parts":[[2023,12,26]]},"reference":[{"issue":"4","key":"R1-20240830","first-page":"224","article-title":"A simple method of genomic DNA extraction from human samples for PCR-RFLP analysis","volume":"24","author":"Ghatak","year":"2013","journal-title":"Journal of Biomolecular 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